Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt NIAGMN_03445 and NIAGMN_03450 overlap by 4 nucleotides NIAGMN_03450 and fhaC are separated by 240 nucleotides fhaC and tnp-IS3 are separated by 180 nucleotides tnp-IS3 and NIAGMN_03465 overlap by 1 nucleotides
NIAGMN_03445: NIAGMN_03445 - helicase, at 617,235 to 619,403
_03445
NIAGMN_03450: NIAGMN_03450 - Putative UvrD/REP helicase-like protein, at 619,400 to 619,915
_03450
NIAGMN_03455: fhaC - ShlB/FhaC/HecB family hemolysin secretion/activation protein, at 620,156 to 621,202
fhaC
NIAGMN_03460: tnp-IS3 - IS3 family transposase, at 621,383 to 621,709
tnp-IS3
NIAGMN_03465: NIAGMN_03465 - IS3 family transposase, at 621,709 to 622,596
_03465
Position (kb) 620
621
622 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 619.293 kb on - strand at 620.031 kb on + strand at 620.104 kb on + strand at 620.185 kb on + strand at 620.476 kb on + strand, within fhaC at 620.536 kb on - strand, within fhaC at 620.633 kb on - strand, within fhaC at 620.661 kb on - strand, within fhaC at 620.737 kb on + strand, within fhaC at 620.740 kb on - strand, within fhaC at 620.740 kb on - strand, within fhaC at 620.740 kb on - strand, within fhaC at 620.789 kb on - strand, within fhaC at 620.789 kb on - strand, within fhaC at 620.854 kb on - strand, within fhaC at 620.854 kb on - strand, within fhaC at 621.041 kb on - strand, within fhaC at 621.180 kb on - strand at 621.192 kb on - strand at 621.314 kb on + strand at 621.314 kb on + strand at 621.315 kb on - strand at 621.315 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 619,293 - -1.6 620,031 + +2.7 620,104 + -0.8 620,185 + -1.4 620,476 + fhaC NIAGMN_03455 0.31 -2.6 620,536 - fhaC NIAGMN_03455 0.36 +0.2 620,633 - fhaC NIAGMN_03455 0.46 +0.8 620,661 - fhaC NIAGMN_03455 0.48 -1.6 620,737 + fhaC NIAGMN_03455 0.55 +3.9 620,740 - fhaC NIAGMN_03455 0.56 -0.9 620,740 - fhaC NIAGMN_03455 0.56 -0.4 620,740 - fhaC NIAGMN_03455 0.56 -1.8 620,789 - fhaC NIAGMN_03455 0.60 -1.3 620,789 - fhaC NIAGMN_03455 0.60 +0.9 620,854 - fhaC NIAGMN_03455 0.67 -1.6 620,854 - fhaC NIAGMN_03455 0.67 -0.3 621,041 - fhaC NIAGMN_03455 0.85 +1.0 621,180 - +0.2 621,192 - -2.7 621,314 + -1.1 621,314 + -0.4 621,315 - -0.5 621,315 - -2.2
Or see this region's nucleotide sequence