Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt NIAGMN_03430 and spo0J are separated by 368 nucleotides spo0J and ybdN overlap by 16 nucleotides ybdN and NIAGMN_03445 are separated by 500 nucleotides
NIAGMN_03430: NIAGMN_03430 - hypothetical protein, at 613,866 to 614,510
_03430
NIAGMN_03435: spo0J - transcriptional regulator, at 614,879 to 615,517
spo0J
NIAGMN_03440: ybdN - phosphoadenosine phosphosulfate reductase, at 615,502 to 616,734
ybdN
NIAGMN_03445: NIAGMN_03445 - helicase, at 617,235 to 619,403
_03445
Position (kb) 615
616
617 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 614.511 kb on + strand at 614.546 kb on - strand at 614.846 kb on - strand at 614.859 kb on - strand at 614.862 kb on - strand at 614.863 kb on + strand at 614.864 kb on - strand at 614.864 kb on - strand at 614.864 kb on - strand at 615.098 kb on - strand, within spo0J at 615.098 kb on - strand, within spo0J at 615.220 kb on - strand, within spo0J at 615.220 kb on - strand, within spo0J at 615.325 kb on - strand, within spo0J at 615.325 kb on - strand, within spo0J at 615.469 kb on - strand at 615.548 kb on + strand at 615.549 kb on - strand at 615.558 kb on - strand at 615.614 kb on - strand at 615.616 kb on - strand at 615.616 kb on - strand at 615.616 kb on - strand at 615.616 kb on - strand at 615.616 kb on - strand at 615.624 kb on + strand at 615.624 kb on + strand at 615.726 kb on - strand, within ybdN at 615.742 kb on - strand, within ybdN at 615.742 kb on - strand, within ybdN at 615.795 kb on - strand, within ybdN at 615.829 kb on - strand, within ybdN at 616.020 kb on - strand, within ybdN at 616.043 kb on + strand, within ybdN at 616.067 kb on + strand, within ybdN at 616.068 kb on - strand, within ybdN at 616.068 kb on - strand, within ybdN at 616.070 kb on - strand, within ybdN at 616.164 kb on - strand, within ybdN at 616.476 kb on - strand, within ybdN at 616.498 kb on - strand, within ybdN at 616.634 kb on - strand at 616.927 kb on - strand at 617.120 kb on + strand at 617.428 kb on + strand at 617.429 kb on - strand at 617.460 kb on - strand, within NIAGMN_03445 at 617.470 kb on - strand, within NIAGMN_03445
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 614,511 + -1.4 614,546 - -0.9 614,846 - -1.4 614,859 - +1.8 614,862 - -1.6 614,863 + -0.4 614,864 - -0.4 614,864 - -1.1 614,864 - +2.5 615,098 - spo0J NIAGMN_03435 0.34 -2.6 615,098 - spo0J NIAGMN_03435 0.34 +2.8 615,220 - spo0J NIAGMN_03435 0.53 +0.7 615,220 - spo0J NIAGMN_03435 0.53 +0.6 615,325 - spo0J NIAGMN_03435 0.70 -0.9 615,325 - spo0J NIAGMN_03435 0.70 +0.3 615,469 - +1.0 615,548 + -2.3 615,549 - -0.2 615,558 - -1.9 615,614 - +0.7 615,616 - -1.2 615,616 - +0.5 615,616 - -0.5 615,616 - -0.9 615,616 - +1.0 615,624 + +0.5 615,624 + -1.4 615,726 - ybdN NIAGMN_03440 0.18 +2.8 615,742 - ybdN NIAGMN_03440 0.19 -1.1 615,742 - ybdN NIAGMN_03440 0.19 +1.7 615,795 - ybdN NIAGMN_03440 0.24 -3.3 615,829 - ybdN NIAGMN_03440 0.27 +2.1 616,020 - ybdN NIAGMN_03440 0.42 -0.6 616,043 + ybdN NIAGMN_03440 0.44 +2.7 616,067 + ybdN NIAGMN_03440 0.46 +0.6 616,068 - ybdN NIAGMN_03440 0.46 +0.9 616,068 - ybdN NIAGMN_03440 0.46 -0.9 616,070 - ybdN NIAGMN_03440 0.46 -0.6 616,164 - ybdN NIAGMN_03440 0.54 +0.3 616,476 - ybdN NIAGMN_03440 0.79 -1.7 616,498 - ybdN NIAGMN_03440 0.81 -2.6 616,634 - -0.0 616,927 - +1.3 617,120 + +1.1 617,428 + -1.6 617,429 - -0.5 617,460 - NIAGMN_03445 0.10 -2.1 617,470 - NIAGMN_03445 0.11 -0.2
Or see this region's nucleotide sequence