Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yeeS and NIAGMN_03365 are separated by 15 nucleotides NIAGMN_03365 and NIAGMN_03370 are separated by 90 nucleotides NIAGMN_03370 and NIAGMN_03375 are separated by 339 nucleotides
NIAGMN_03360: yeeS - UPF0758 protein YeeS, at 600,847 to 601,323
yeeS
NIAGMN_03365: NIAGMN_03365 - restriction endonuclease, at 601,339 to 601,824
_03365
NIAGMN_03370: NIAGMN_03370 - DUF945 domain-containing protein, at 601,915 to 602,733
_03370
NIAGMN_03375: NIAGMN_03375 - phospholipase, at 603,073 to 604,143
_03375
Position (kb) 601
602
603 Strain fitness (log2 ratio) -2
-1
0
1
2 at 601.455 kb on - strand, within NIAGMN_03365 at 601.599 kb on - strand, within NIAGMN_03365 at 602.365 kb on - strand, within NIAGMN_03370 at 602.365 kb on - strand, within NIAGMN_03370 at 602.399 kb on + strand, within NIAGMN_03370 at 602.597 kb on + strand, within NIAGMN_03370 at 602.597 kb on + strand, within NIAGMN_03370 at 602.598 kb on - strand, within NIAGMN_03370 at 602.603 kb on + strand, within NIAGMN_03370 at 602.650 kb on + strand, within NIAGMN_03370 at 602.746 kb on - strand at 602.762 kb on + strand at 602.826 kb on - strand at 602.859 kb on + strand at 602.860 kb on - strand at 602.978 kb on - strand at 602.993 kb on - strand at 602.993 kb on - strand at 602.996 kb on - strand at 602.998 kb on - strand at 602.998 kb on - strand at 602.998 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 601,455 - NIAGMN_03365 0.24 -0.2 601,599 - NIAGMN_03365 0.53 -1.9 602,365 - NIAGMN_03370 0.55 -1.4 602,365 - NIAGMN_03370 0.55 +0.8 602,399 + NIAGMN_03370 0.59 +2.5 602,597 + NIAGMN_03370 0.83 -0.4 602,597 + NIAGMN_03370 0.83 -1.3 602,598 - NIAGMN_03370 0.83 +1.7 602,603 + NIAGMN_03370 0.84 +0.7 602,650 + NIAGMN_03370 0.90 -0.5 602,746 - +2.4 602,762 + -0.6 602,826 - -1.1 602,859 + -0.1 602,860 - +0.9 602,978 - +0.4 602,993 - +0.1 602,993 - -0.0 602,996 - -0.8 602,998 - -0.4 602,998 - +1.8 602,998 - +0.2
Or see this region's nucleotide sequence