Strain Fitness in Escherichia coli ECRC102 around NIAGMN_03095

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpncB and asnS are separated by 168 nucleotidesasnS and ompF are separated by 602 nucleotides NIAGMN_03090: pncB - nicotinate phosphoribosyltransferase, at 534,965 to 536,167 pncB NIAGMN_03095: asnS - asparagine--tRNA ligase, at 536,336 to 537,736 asnS NIAGMN_03100: ompF - porin OmpF, at 538,339 to 539,427 ompF Position (kb) 536 537 538Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 535.425 kb on + strand, within pncBat 535.459 kb on + strand, within pncBat 535.459 kb on + strand, within pncBat 535.459 kb on + strand, within pncBat 535.574 kb on + strand, within pncBat 535.662 kb on + strand, within pncBat 535.747 kb on + strand, within pncBat 535.747 kb on + strand, within pncBat 535.747 kb on + strand, within pncBat 535.965 kb on + strand, within pncBat 535.965 kb on + strand, within pncBat 536.023 kb on + strand, within pncBat 536.023 kb on + strand, within pncBat 536.023 kb on + strand, within pncBat 536.023 kb on + strand, within pncBat 536.032 kb on + strand, within pncBat 536.032 kb on + strand, within pncBat 536.032 kb on + strand, within pncBat 536.040 kb on + strand, within pncBat 536.092 kb on + strandat 536.104 kb on + strandat 536.152 kb on + strandat 536.165 kb on + strandat 536.165 kb on + strandat 536.165 kb on + strandat 536.270 kb on + strandat 536.274 kb on + strandat 537.794 kb on - strandat 537.805 kb on - strandat 538.101 kb on - strandat 538.236 kb on + strandat 538.383 kb on - strandat 538.385 kb on + strandat 538.468 kb on + strand, within ompFat 538.522 kb on + strand, within ompFat 538.539 kb on + strand, within ompFat 538.591 kb on + strand, within ompFat 538.593 kb on + strand, within ompFat 538.605 kb on + strand, within ompFat 538.672 kb on + strand, within ompFat 538.708 kb on + strand, within ompFat 538.708 kb on + strand, within ompFat 538.735 kb on + strand, within ompFat 538.735 kb on + strand, within ompFat 538.735 kb on + strand, within ompF

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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535,425 + pncB NIAGMN_03090 0.38 -0.3
535,459 + pncB NIAGMN_03090 0.41 +1.0
535,459 + pncB NIAGMN_03090 0.41 -1.1
535,459 + pncB NIAGMN_03090 0.41 -1.4
535,574 + pncB NIAGMN_03090 0.51 -0.1
535,662 + pncB NIAGMN_03090 0.58 -1.7
535,747 + pncB NIAGMN_03090 0.65 +1.2
535,747 + pncB NIAGMN_03090 0.65 +0.5
535,747 + pncB NIAGMN_03090 0.65 -2.3
535,965 + pncB NIAGMN_03090 0.83 +2.5
535,965 + pncB NIAGMN_03090 0.83 +0.4
536,023 + pncB NIAGMN_03090 0.88 +0.0
536,023 + pncB NIAGMN_03090 0.88 +0.9
536,023 + pncB NIAGMN_03090 0.88 +0.3
536,023 + pncB NIAGMN_03090 0.88 +0.9
536,032 + pncB NIAGMN_03090 0.89 -1.8
536,032 + pncB NIAGMN_03090 0.89 -1.4
536,032 + pncB NIAGMN_03090 0.89 -1.0
536,040 + pncB NIAGMN_03090 0.89 -2.6
536,092 + +0.8
536,104 + -0.6
536,152 + -1.5
536,165 + +0.7
536,165 + -1.4
536,165 + +2.0
536,270 + -1.0
536,274 + -2.0
537,794 - -0.1
537,805 - +0.9
538,101 - -2.7
538,236 + -1.2
538,383 - +0.9
538,385 + +1.5
538,468 + ompF NIAGMN_03100 0.12 +1.2
538,522 + ompF NIAGMN_03100 0.17 -0.8
538,539 + ompF NIAGMN_03100 0.18 -1.4
538,591 + ompF NIAGMN_03100 0.23 -0.4
538,593 + ompF NIAGMN_03100 0.23 -2.0
538,605 + ompF NIAGMN_03100 0.24 +0.1
538,672 + ompF NIAGMN_03100 0.31 -1.5
538,708 + ompF NIAGMN_03100 0.34 +2.3
538,708 + ompF NIAGMN_03100 0.34 -0.2
538,735 + ompF NIAGMN_03100 0.36 +0.3
538,735 + ompF NIAGMN_03100 0.36 -1.0
538,735 + ompF NIAGMN_03100 0.36 -3.0

Or see this region's nucleotide sequence