Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ssuC and ssuB overlap by 4 nucleotides ssuB and pepN are separated by 42 nucleotides pepN and pncB are separated by 265 nucleotides
NIAGMN_03075: ssuC - aliphatic sulfonate ABC transporter permease SsuC, at 530,489 to 531,280
ssuC
NIAGMN_03080: ssuB - aliphatic sulfonates ABC transporter ATP-binding protein, at 531,277 to 532,044
ssuB
NIAGMN_03085: pepN - aminopeptidase N, at 532,087 to 534,699
pepN
NIAGMN_03090: pncB - nicotinate phosphoribosyltransferase, at 534,965 to 536,167
pncB
Position (kb) 532
533
534
535 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 531.172 kb on + strand, within ssuC at 531.283 kb on - strand at 531.337 kb on + strand at 531.371 kb on + strand, within ssuB at 531.371 kb on + strand, within ssuB at 531.574 kb on - strand, within ssuB at 531.828 kb on + strand, within ssuB at 531.890 kb on + strand, within ssuB at 532.086 kb on - strand at 532.193 kb on + strand at 532.235 kb on - strand at 532.589 kb on + strand, within pepN at 532.657 kb on - strand, within pepN at 532.657 kb on - strand, within pepN at 532.792 kb on - strand, within pepN at 532.921 kb on + strand, within pepN at 532.977 kb on - strand, within pepN at 533.068 kb on - strand, within pepN at 533.068 kb on - strand, within pepN at 533.070 kb on - strand, within pepN at 533.127 kb on - strand, within pepN at 533.838 kb on - strand, within pepN at 534.074 kb on - strand, within pepN at 534.113 kb on - strand, within pepN at 534.504 kb on + strand at 534.505 kb on - strand at 534.721 kb on - strand at 534.721 kb on - strand at 534.723 kb on - strand at 534.723 kb on - strand at 534.742 kb on - strand at 534.742 kb on - strand at 534.742 kb on - strand at 534.755 kb on - strand at 534.755 kb on - strand at 534.790 kb on - strand at 534.822 kb on + strand at 534.823 kb on - strand at 534.823 kb on - strand at 534.823 kb on - strand at 534.831 kb on + strand at 534.858 kb on - strand at 534.858 kb on - strand at 534.858 kb on - strand at 534.858 kb on - strand at 534.896 kb on - strand at 534.897 kb on + strand at 534.897 kb on + strand at 534.930 kb on + strand at 534.964 kb on + strand at 534.964 kb on + strand at 534.964 kb on + strand at 535.016 kb on + strand at 535.017 kb on - strand at 535.017 kb on - strand at 535.018 kb on + strand at 535.018 kb on + strand at 535.018 kb on + strand at 535.018 kb on + strand at 535.018 kb on + strand at 535.018 kb on + strand at 535.019 kb on - strand at 535.019 kb on - strand at 535.019 kb on - strand at 535.052 kb on + strand at 535.055 kb on + strand at 535.055 kb on + strand at 535.056 kb on - strand at 535.056 kb on - strand at 535.108 kb on + strand, within pncB at 535.112 kb on + strand, within pncB at 535.175 kb on + strand, within pncB at 535.219 kb on + strand, within pncB at 535.219 kb on + strand, within pncB at 535.219 kb on + strand, within pncB at 535.425 kb on + strand, within pncB at 535.459 kb on + strand, within pncB at 535.459 kb on + strand, within pncB at 535.459 kb on + strand, within pncB at 535.574 kb on + strand, within pncB at 535.662 kb on + strand, within pncB
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 531,172 + ssuC NIAGMN_03075 0.86 +1.2 531,283 - -1.9 531,337 + +1.1 531,371 + ssuB NIAGMN_03080 0.12 -0.5 531,371 + ssuB NIAGMN_03080 0.12 +2.2 531,574 - ssuB NIAGMN_03080 0.39 -0.0 531,828 + ssuB NIAGMN_03080 0.72 +0.7 531,890 + ssuB NIAGMN_03080 0.80 +3.2 532,086 - +0.3 532,193 + -1.2 532,235 - -0.9 532,589 + pepN NIAGMN_03085 0.19 -2.9 532,657 - pepN NIAGMN_03085 0.22 -1.0 532,657 - pepN NIAGMN_03085 0.22 +0.9 532,792 - pepN NIAGMN_03085 0.27 -1.2 532,921 + pepN NIAGMN_03085 0.32 +1.0 532,977 - pepN NIAGMN_03085 0.34 +2.4 533,068 - pepN NIAGMN_03085 0.38 +0.0 533,068 - pepN NIAGMN_03085 0.38 -1.1 533,070 - pepN NIAGMN_03085 0.38 -1.4 533,127 - pepN NIAGMN_03085 0.40 +2.0 533,838 - pepN NIAGMN_03085 0.67 -0.5 534,074 - pepN NIAGMN_03085 0.76 +1.8 534,113 - pepN NIAGMN_03085 0.78 -0.0 534,504 + -0.4 534,505 - +0.4 534,721 - -0.5 534,721 - +0.2 534,723 - +1.1 534,723 - +0.8 534,742 - +2.0 534,742 - -2.5 534,742 - +0.2 534,755 - +2.6 534,755 - +0.5 534,790 - -2.3 534,822 + +1.4 534,823 - -2.9 534,823 - +0.2 534,823 - -3.2 534,831 + -0.6 534,858 - +1.4 534,858 - +0.0 534,858 - +1.2 534,858 - +0.3 534,896 - +3.7 534,897 + +0.1 534,897 + -0.6 534,930 + -2.1 534,964 + -0.4 534,964 + -2.0 534,964 + +0.5 535,016 + -4.1 535,017 - -1.8 535,017 - -1.5 535,018 + +0.4 535,018 + -2.5 535,018 + +1.3 535,018 + -2.5 535,018 + +0.5 535,018 + -1.1 535,019 - +2.3 535,019 - -0.4 535,019 - -1.4 535,052 + +2.5 535,055 + -1.0 535,055 + +0.2 535,056 - +2.3 535,056 - +0.3 535,108 + pncB NIAGMN_03090 0.12 -0.7 535,112 + pncB NIAGMN_03090 0.12 -1.9 535,175 + pncB NIAGMN_03090 0.17 -1.4 535,219 + pncB NIAGMN_03090 0.21 -2.2 535,219 + pncB NIAGMN_03090 0.21 +0.3 535,219 + pncB NIAGMN_03090 0.21 -0.4 535,425 + pncB NIAGMN_03090 0.38 -0.3 535,459 + pncB NIAGMN_03090 0.41 +1.0 535,459 + pncB NIAGMN_03090 0.41 -1.1 535,459 + pncB NIAGMN_03090 0.41 -1.4 535,574 + pncB NIAGMN_03090 0.51 -0.1 535,662 + pncB NIAGMN_03090 0.58 -1.7
Or see this region's nucleotide sequence