Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt NIAGMN_02805 and NIAGMN_02810 are separated by 78 nucleotides NIAGMN_02810 and NIAGMN_02815 are separated by 206 nucleotides NIAGMN_02815 and NIAGMN_02820 overlap by 17 nucleotides NIAGMN_02820 and hipB are separated by 108 nucleotides hipB and NIAGMN_02830 are separated by 27 nucleotides
NIAGMN_02805: NIAGMN_02805 - DNA-binding protein, at 483,808 to 484,899
_02805
NIAGMN_02810: NIAGMN_02810 - hypothetical protein, at 484,978 to 485,433
_02810
NIAGMN_02815: NIAGMN_02815 - Rha family transcriptional regulator, at 485,640 to 486,065
_02815
NIAGMN_02820: NIAGMN_02820 - Putative antitoxin of bacterial toxin-antitoxin system, YdaS/YdaT, at 486,049 to 486,321
_02820
NIAGMN_02825: hipB - XRE family transcriptional regulator, at 486,430 to 486,831
hipB
NIAGMN_02830: NIAGMN_02830 - antitoxin, at 486,859 to 487,050
_02830
Position (kb)
485
486
487 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 484.664 kb on + strand, within NIAGMN_02805 at 484.665 kb on - strand, within NIAGMN_02805 at 484.665 kb on - strand, within NIAGMN_02805 at 484.696 kb on - strand, within NIAGMN_02805 at 484.748 kb on - strand, within NIAGMN_02805 at 484.764 kb on - strand, within NIAGMN_02805 at 484.764 kb on - strand, within NIAGMN_02805 at 484.764 kb on - strand, within NIAGMN_02805 at 484.777 kb on - strand, within NIAGMN_02805 at 484.777 kb on - strand, within NIAGMN_02805 at 484.840 kb on - strand at 484.840 kb on - strand at 484.840 kb on - strand at 484.840 kb on - strand at 484.840 kb on - strand at 484.903 kb on + strand at 484.936 kb on + strand at 484.937 kb on - strand at 484.937 kb on - strand at 484.937 kb on - strand at 484.937 kb on - strand at 484.982 kb on - strand at 485.431 kb on + strand at 485.528 kb on + strand at 486.138 kb on + strand, within NIAGMN_02820 at 486.312 kb on + strand at 486.853 kb on + strand at 487.031 kb on + strand at 487.031 kb on + strand at 487.035 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 484,664 + NIAGMN_02805 0.78 +0.8 484,665 - NIAGMN_02805 0.78 +3.5 484,665 - NIAGMN_02805 0.78 +1.6 484,696 - NIAGMN_02805 0.81 -2.3 484,748 - NIAGMN_02805 0.86 +0.2 484,764 - NIAGMN_02805 0.88 -3.6 484,764 - NIAGMN_02805 0.88 -1.7 484,764 - NIAGMN_02805 0.88 +1.4 484,777 - NIAGMN_02805 0.89 +1.7 484,777 - NIAGMN_02805 0.89 -2.3 484,840 - -1.4 484,840 - -2.0 484,840 - -2.9 484,840 - +0.2 484,840 - -0.2 484,903 + +3.4 484,936 + +3.7 484,937 - +1.1 484,937 - -1.3 484,937 - +0.7 484,937 - -0.3 484,982 - -1.2 485,431 + +0.8 485,528 + +1.9 486,138 + NIAGMN_02820 0.33 +0.2 486,312 + +0.4 486,853 + -0.8 487,031 + +0.2 487,031 + +0.6 487,035 + +2.0
Or see this region's nucleotide sequence