Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yedE and yedF overlap by 4 nucleotides yedF and yedK are separated by 108 nucleotides yedK and yedL are separated by 110 nucleotides yedL and espR3 are separated by 137 nucleotides
NIAGMN_02305: yedE - selenium metabolism membrane protein YedE/FdhT, at 406,387 to 407,592
yedE
NIAGMN_02310: yedF - sulfurtransferase-like selenium metabolism protein YedF, at 407,589 to 407,822
yedF
NIAGMN_02315: yedK - Abasic site processing protein YedK, at 407,931 to 408,599
yedK
NIAGMN_02320: yedL - Uncharacterized N-acetyltransferase YedL, at 408,710 to 409,189
yedL
NIAGMN_02325: espR3 - Type III secretion system effector espR3, at 409,327 to 410,466
espR3
Position (kb) 407
408
409 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 407.149 kb on - strand, within yedE at 407.154 kb on + strand, within yedE at 407.209 kb on + strand, within yedE at 407.213 kb on + strand, within yedE at 407.213 kb on + strand, within yedE at 407.273 kb on + strand, within yedE at 407.326 kb on + strand, within yedE at 407.326 kb on + strand, within yedE at 407.401 kb on + strand, within yedE at 407.805 kb on + strand at 407.821 kb on - strand at 407.870 kb on - strand at 407.912 kb on - strand at 407.931 kb on - strand at 407.931 kb on - strand at 408.037 kb on - strand, within yedK at 408.186 kb on - strand, within yedK at 408.186 kb on - strand, within yedK at 408.400 kb on + strand, within yedK
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 407,149 - yedE NIAGMN_02305 0.63 -0.1 407,154 + yedE NIAGMN_02305 0.64 +0.8 407,209 + yedE NIAGMN_02305 0.68 +2.9 407,213 + yedE NIAGMN_02305 0.68 +0.0 407,213 + yedE NIAGMN_02305 0.68 -0.9 407,273 + yedE NIAGMN_02305 0.73 +1.6 407,326 + yedE NIAGMN_02305 0.78 -0.3 407,326 + yedE NIAGMN_02305 0.78 -3.1 407,401 + yedE NIAGMN_02305 0.84 -1.0 407,805 + -1.9 407,821 - -3.1 407,870 - +2.0 407,912 - -1.8 407,931 - -0.1 407,931 - +0.6 408,037 - yedK NIAGMN_02315 0.16 -0.6 408,186 - yedK NIAGMN_02315 0.38 +0.1 408,186 - yedK NIAGMN_02315 0.38 -1.6 408,400 + yedK NIAGMN_02315 0.70 +2.4
Or see this region's nucleotide sequence