Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliD and fliS are separated by 24 nucleotides fliS and fliT overlap by 1 nucleotides fliT and amyA are separated by 77 nucleotides amyA and yedD are separated by 33 nucleotides yedD and yedE are separated by 186 nucleotides
NIAGMN_02280: fliD - flagellar filament capping protein FliD, at 401,991 to 403,388
fliD
NIAGMN_02285: fliS - flagellar export chaperone FliS, at 403,413 to 403,823
fliS
NIAGMN_02290: fliT - flagella biosynthesis regulatory protein FliT, at 403,823 to 404,188
fliT
NIAGMN_02295: amyA - alpha-amylase, at 404,266 to 405,753
amyA
NIAGMN_02300: yedD - lipoprotein, at 405,787 to 406,200
yedD
NIAGMN_02305: yedE - selenium metabolism membrane protein YedE/FdhT, at 406,387 to 407,592
yedE
Position (kb) 404
405
406 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 403.348 kb on - strand at 403.351 kb on + strand at 403.377 kb on - strand at 403.386 kb on + strand at 403.416 kb on + strand at 403.441 kb on - strand at 403.504 kb on + strand, within fliS at 403.683 kb on + strand, within fliS at 403.683 kb on + strand, within fliS at 403.683 kb on + strand, within fliS at 403.683 kb on + strand, within fliS at 403.684 kb on - strand, within fliS at 403.684 kb on - strand, within fliS at 403.685 kb on + strand, within fliS at 403.940 kb on + strand, within fliT at 403.940 kb on + strand, within fliT at 404.238 kb on + strand at 404.371 kb on - strand at 404.376 kb on + strand at 404.377 kb on - strand at 404.401 kb on - strand at 404.490 kb on + strand, within amyA at 405.429 kb on + strand, within amyA at 405.630 kb on + strand at 405.630 kb on + strand at 405.671 kb on + strand at 405.871 kb on - strand, within yedD at 405.871 kb on - strand, within yedD at 405.879 kb on - strand, within yedD at 405.879 kb on - strand, within yedD at 405.879 kb on - strand, within yedD at 406.007 kb on - strand, within yedD at 406.337 kb on + strand at 406.475 kb on - strand at 406.590 kb on + strand, within yedE at 406.695 kb on - strand, within yedE at 406.735 kb on - strand, within yedE
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 403,348 - -1.7 403,351 + -0.0 403,377 - -0.3 403,386 + +2.5 403,416 + -3.6 403,441 - -1.0 403,504 + fliS NIAGMN_02285 0.22 -0.1 403,683 + fliS NIAGMN_02285 0.66 +0.1 403,683 + fliS NIAGMN_02285 0.66 +0.5 403,683 + fliS NIAGMN_02285 0.66 -0.1 403,683 + fliS NIAGMN_02285 0.66 -0.3 403,684 - fliS NIAGMN_02285 0.66 -2.0 403,684 - fliS NIAGMN_02285 0.66 -0.6 403,685 + fliS NIAGMN_02285 0.66 +1.6 403,940 + fliT NIAGMN_02290 0.32 -1.2 403,940 + fliT NIAGMN_02290 0.32 -0.2 404,238 + -0.5 404,371 - -0.7 404,376 + +0.9 404,377 - +3.7 404,401 - +0.8 404,490 + amyA NIAGMN_02295 0.15 +2.5 405,429 + amyA NIAGMN_02295 0.78 +3.4 405,630 + -0.4 405,630 + -0.9 405,671 + +1.3 405,871 - yedD NIAGMN_02300 0.20 -1.2 405,871 - yedD NIAGMN_02300 0.20 -0.3 405,879 - yedD NIAGMN_02300 0.22 +0.4 405,879 - yedD NIAGMN_02300 0.22 -2.4 405,879 - yedD NIAGMN_02300 0.22 -0.8 406,007 - yedD NIAGMN_02300 0.53 +0.7 406,337 + -2.0 406,475 - +0.3 406,590 + yedE NIAGMN_02305 0.17 -0.7 406,695 - yedE NIAGMN_02305 0.26 -0.3 406,735 - yedE NIAGMN_02305 0.29 +1.5
Or see this region's nucleotide sequence