Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aspS and yecD are separated by 309 nucleotides yecD and yecE overlap by 4 nucleotides yecE and yecN are separated by 52 nucleotides yecN and cmoA are separated by 40 nucleotides
NIAGMN_01815: aspS - aspartate--tRNA ligase, at 326,262 to 328,034
aspS
NIAGMN_01820: yecD - hydrolase, at 328,344 to 328,910
yecD
NIAGMN_01825: yecE - UPF0759 protein YecE, at 328,907 to 329,725
yecE
NIAGMN_01830: yecN - Inner membrane protein YecN, at 329,778 to 330,173
yecN
NIAGMN_01835: cmoA - carboxy-S-adenosyl-L-methionine synthase CmoA, at 330,214 to 330,957
cmoA
Position (kb) 328
329
330 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 328.202 kb on - strand at 328.225 kb on + strand at 328.241 kb on - strand at 328.262 kb on - strand at 328.355 kb on + strand at 328.356 kb on - strand at 328.356 kb on - strand at 328.427 kb on + strand, within yecD at 328.441 kb on + strand, within yecD at 328.441 kb on + strand, within yecD at 328.441 kb on + strand, within yecD at 328.541 kb on - strand, within yecD at 328.613 kb on - strand, within yecD at 328.709 kb on + strand, within yecD at 328.709 kb on + strand, within yecD at 328.720 kb on + strand, within yecD at 328.720 kb on + strand, within yecD at 328.785 kb on - strand, within yecD at 328.838 kb on + strand, within yecD at 328.845 kb on - strand, within yecD at 328.853 kb on + strand, within yecD at 328.980 kb on - strand at 329.286 kb on - strand, within yecE at 329.475 kb on + strand, within yecE at 329.605 kb on - strand, within yecE at 329.609 kb on - strand, within yecE at 329.618 kb on - strand, within yecE at 329.644 kb on + strand at 329.645 kb on - strand at 329.661 kb on + strand at 329.662 kb on - strand at 329.666 kb on - strand at 329.736 kb on + strand at 329.736 kb on + strand at 329.737 kb on - strand at 329.737 kb on - strand at 329.737 kb on - strand at 329.934 kb on + strand, within yecN at 329.934 kb on + strand, within yecN at 329.935 kb on - strand, within yecN at 329.936 kb on + strand, within yecN at 330.141 kb on - strand at 330.141 kb on - strand at 330.141 kb on - strand at 330.339 kb on + strand, within cmoA at 330.565 kb on - strand, within cmoA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 328,202 - +2.1 328,225 + -1.5 328,241 - -1.6 328,262 - +1.4 328,355 + +0.9 328,356 - -2.2 328,356 - +0.8 328,427 + yecD NIAGMN_01820 0.15 +2.0 328,441 + yecD NIAGMN_01820 0.17 +1.5 328,441 + yecD NIAGMN_01820 0.17 -0.9 328,441 + yecD NIAGMN_01820 0.17 +1.0 328,541 - yecD NIAGMN_01820 0.35 +0.3 328,613 - yecD NIAGMN_01820 0.47 -1.0 328,709 + yecD NIAGMN_01820 0.64 -1.3 328,709 + yecD NIAGMN_01820 0.64 -1.4 328,720 + yecD NIAGMN_01820 0.66 +1.6 328,720 + yecD NIAGMN_01820 0.66 +2.5 328,785 - yecD NIAGMN_01820 0.78 +3.4 328,838 + yecD NIAGMN_01820 0.87 +0.8 328,845 - yecD NIAGMN_01820 0.88 -0.9 328,853 + yecD NIAGMN_01820 0.90 +0.3 328,980 - -0.3 329,286 - yecE NIAGMN_01825 0.46 +1.7 329,475 + yecE NIAGMN_01825 0.69 -1.9 329,605 - yecE NIAGMN_01825 0.85 +0.4 329,609 - yecE NIAGMN_01825 0.86 -2.8 329,618 - yecE NIAGMN_01825 0.87 +0.9 329,644 + +0.0 329,645 - +3.7 329,661 + -0.0 329,662 - +0.5 329,666 - +2.1 329,736 + -0.9 329,736 + -0.9 329,737 - +1.4 329,737 - -0.8 329,737 - -0.3 329,934 + yecN NIAGMN_01830 0.39 -1.8 329,934 + yecN NIAGMN_01830 0.39 +0.7 329,935 - yecN NIAGMN_01830 0.40 -4.1 329,936 + yecN NIAGMN_01830 0.40 +2.4 330,141 - +4.2 330,141 - -2.6 330,141 - +1.1 330,339 + cmoA NIAGMN_01835 0.17 +2.1 330,565 - cmoA NIAGMN_01835 0.47 +0.3
Or see this region's nucleotide sequence