Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt copD and yobA are separated by 3 nucleotides yobA and holE are separated by 138 nucleotides holE and yobB are separated by 101 nucleotides yobB and exoX are separated by 23 nucleotides
NIAGMN_01655: copD - copper homeostasis membrane protein CopD, at 301,132 to 302,004
copD
NIAGMN_01660: yobA - CopC domain-containing protein YobA, at 302,008 to 302,382
yobA
NIAGMN_01665: holE - DNA polymerase III subunit theta, at 302,521 to 302,751
holE
NIAGMN_01670: yobB - Uncharacterized protein YobB, at 302,853 to 303,509
yobB
NIAGMN_01675: exoX - exodeoxyribonuclease X, at 303,533 to 304,195
exoX
Position (kb) 302
303 Strain fitness (log2 ratio) -2
-1
0
1 at 301.636 kb on - strand, within copD at 301.645 kb on - strand, within copD at 301.645 kb on - strand, within copD at 301.662 kb on + strand, within copD at 301.696 kb on + strand, within copD at 301.929 kb on - strand at 302.027 kb on + strand at 302.028 kb on - strand at 302.028 kb on - strand at 302.081 kb on + strand, within yobA at 302.081 kb on + strand, within yobA at 302.082 kb on - strand, within yobA at 302.082 kb on - strand, within yobA at 302.164 kb on - strand, within yobA at 302.283 kb on - strand, within yobA at 302.382 kb on + strand at 302.385 kb on + strand at 302.385 kb on + strand at 302.386 kb on - strand at 302.386 kb on - strand at 302.422 kb on + strand at 302.612 kb on - strand, within holE at 302.616 kb on + strand, within holE at 302.617 kb on - strand, within holE at 302.617 kb on - strand, within holE at 302.617 kb on - strand, within holE at 303.178 kb on - strand, within yobB at 303.454 kb on + strand at 303.658 kb on + strand, within exoX at 303.743 kb on + strand, within exoX
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 301,636 - copD NIAGMN_01655 0.58 -0.3 301,645 - copD NIAGMN_01655 0.59 -2.2 301,645 - copD NIAGMN_01655 0.59 +1.2 301,662 + copD NIAGMN_01655 0.61 -2.5 301,696 + copD NIAGMN_01655 0.65 +0.6 301,929 - +1.0 302,027 + -0.1 302,028 - -1.6 302,028 - +0.5 302,081 + yobA NIAGMN_01660 0.19 +0.4 302,081 + yobA NIAGMN_01660 0.19 +0.4 302,082 - yobA NIAGMN_01660 0.20 -2.2 302,082 - yobA NIAGMN_01660 0.20 -2.4 302,164 - yobA NIAGMN_01660 0.42 -1.1 302,283 - yobA NIAGMN_01660 0.73 +1.7 302,382 + -0.2 302,385 + -1.8 302,385 + +0.9 302,386 - +0.2 302,386 - -0.3 302,422 + -2.0 302,612 - holE NIAGMN_01665 0.39 +1.4 302,616 + holE NIAGMN_01665 0.41 +0.8 302,617 - holE NIAGMN_01665 0.42 +0.3 302,617 - holE NIAGMN_01665 0.42 +0.3 302,617 - holE NIAGMN_01665 0.42 -0.6 303,178 - yobB NIAGMN_01670 0.49 -0.2 303,454 + -2.2 303,658 + exoX NIAGMN_01675 0.19 -0.5 303,743 + exoX NIAGMN_01675 0.32 +0.8
Or see this region's nucleotide sequence