Strain Fitness in Escherichia coli ECRC102 around NIAGMN_01265

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntansA and pncA are separated by 10 nucleotidespncA and ydjE are separated by 92 nucleotides NIAGMN_01260: ansA - asparaginase, at 226,735 to 227,751 ansA NIAGMN_01265: pncA - bifunctional nicotinamidase/pyrazinamidase, at 227,762 to 228,403 pncA NIAGMN_01270: ydjE - Inner membrane metabolite transport protein YdjE, at 228,496 to 229,656 ydjE Position (kb) 227 228 229Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 226.806 kb on + strandat 226.944 kb on + strand, within ansAat 227.105 kb on + strand, within ansAat 227.346 kb on - strand, within ansAat 227.553 kb on + strand, within ansAat 227.553 kb on + strand, within ansAat 227.553 kb on + strand, within ansAat 227.971 kb on + strand, within pncAat 227.972 kb on - strand, within pncAat 227.972 kb on - strand, within pncAat 228.268 kb on + strand, within pncAat 228.366 kb on + strandat 228.366 kb on + strandat 228.370 kb on + strandat 228.370 kb on + strandat 228.370 kb on + strandat 228.371 kb on - strandat 228.402 kb on - strandat 228.447 kb on + strandat 228.706 kb on + strand, within ydjEat 228.759 kb on - strand, within ydjEat 229.117 kb on + strand, within ydjEat 229.196 kb on - strand, within ydjE

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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226,806 + -0.1
226,944 + ansA NIAGMN_01260 0.21 +1.0
227,105 + ansA NIAGMN_01260 0.36 +1.9
227,346 - ansA NIAGMN_01260 0.60 -2.0
227,553 + ansA NIAGMN_01260 0.80 -0.7
227,553 + ansA NIAGMN_01260 0.80 +1.7
227,553 + ansA NIAGMN_01260 0.80 +1.7
227,971 + pncA NIAGMN_01265 0.33 +1.5
227,972 - pncA NIAGMN_01265 0.33 +0.4
227,972 - pncA NIAGMN_01265 0.33 +0.1
228,268 + pncA NIAGMN_01265 0.79 +0.3
228,366 + +0.1
228,366 + -0.1
228,370 + -0.6
228,370 + +1.8
228,370 + -3.5
228,371 - -3.1
228,402 - -1.7
228,447 + -2.4
228,706 + ydjE NIAGMN_01270 0.18 +0.9
228,759 - ydjE NIAGMN_01270 0.23 -3.3
229,117 + ydjE NIAGMN_01270 0.53 +1.1
229,196 - ydjE NIAGMN_01270 0.60 +0.9

Or see this region's nucleotide sequence