Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yniB and hxpB are separated by 146 nucleotides hxpB and ybcI are separated by 162 nucleotides ybcI and tcyP are separated by 132 nucleotides
NIAGMN_01045: yniB - Uncharacterized protein YniB, at 183,982 to 184,518
yniB
NIAGMN_01050: hxpB - hexitol phosphatase HxpB, at 184,665 to 185,333
hxpB
NIAGMN_01055: ybcI - metal-dependent hydrolase, at 185,496 to 186,086
ybcI
NIAGMN_01060: tcyP - cystine/sulfocysteine:cation symporter, at 186,219 to 187,610
tcyP
Position (kb) 185
186
187 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 184.512 kb on - strand at 184.533 kb on + strand at 184.552 kb on + strand at 184.563 kb on + strand at 184.564 kb on - strand at 184.564 kb on - strand at 184.564 kb on - strand at 184.564 kb on - strand at 184.602 kb on + strand at 184.610 kb on + strand at 184.616 kb on + strand at 184.630 kb on + strand at 184.631 kb on - strand at 184.631 kb on - strand at 184.636 kb on - strand at 184.642 kb on - strand at 184.642 kb on - strand at 184.715 kb on - strand at 184.891 kb on + strand, within hxpB at 185.123 kb on - strand, within hxpB at 185.424 kb on - strand at 185.467 kb on + strand at 185.467 kb on + strand at 185.482 kb on + strand at 185.594 kb on + strand, within ybcI at 185.804 kb on - strand, within ybcI at 185.836 kb on + strand, within ybcI at 185.959 kb on + strand, within ybcI at 185.960 kb on - strand, within ybcI at 186.172 kb on + strand at 186.197 kb on + strand at 186.290 kb on + strand at 186.369 kb on + strand, within tcyP at 186.369 kb on + strand, within tcyP at 186.388 kb on + strand, within tcyP at 186.388 kb on + strand, within tcyP at 186.403 kb on + strand, within tcyP at 186.674 kb on + strand, within tcyP at 186.678 kb on + strand, within tcyP at 186.678 kb on + strand, within tcyP at 186.678 kb on + strand, within tcyP at 186.678 kb on + strand, within tcyP at 186.678 kb on + strand, within tcyP at 186.679 kb on - strand, within tcyP at 186.814 kb on + strand, within tcyP at 186.814 kb on + strand, within tcyP at 186.814 kb on + strand, within tcyP at 186.866 kb on - strand, within tcyP at 187.020 kb on + strand, within tcyP at 187.037 kb on + strand, within tcyP
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 184,512 - -0.1 184,533 + -0.3 184,552 + +0.9 184,563 + -1.4 184,564 - -2.0 184,564 - +4.1 184,564 - +4.3 184,564 - -1.9 184,602 + -0.4 184,610 + -2.7 184,616 + -0.3 184,630 + +0.4 184,631 - +1.1 184,631 - +1.3 184,636 - -2.2 184,642 - +0.8 184,642 - -0.5 184,715 - +0.5 184,891 + hxpB NIAGMN_01050 0.34 +0.1 185,123 - hxpB NIAGMN_01050 0.68 +0.1 185,424 - +0.2 185,467 + +0.5 185,467 + -2.1 185,482 + -1.8 185,594 + ybcI NIAGMN_01055 0.17 +2.0 185,804 - ybcI NIAGMN_01055 0.52 -2.0 185,836 + ybcI NIAGMN_01055 0.58 +1.0 185,959 + ybcI NIAGMN_01055 0.78 +1.6 185,960 - ybcI NIAGMN_01055 0.79 -0.7 186,172 + +1.2 186,197 + +1.4 186,290 + -0.9 186,369 + tcyP NIAGMN_01060 0.11 -3.5 186,369 + tcyP NIAGMN_01060 0.11 +1.0 186,388 + tcyP NIAGMN_01060 0.12 -1.1 186,388 + tcyP NIAGMN_01060 0.12 +0.3 186,403 + tcyP NIAGMN_01060 0.13 +2.4 186,674 + tcyP NIAGMN_01060 0.33 -2.1 186,678 + tcyP NIAGMN_01060 0.33 -0.5 186,678 + tcyP NIAGMN_01060 0.33 -2.4 186,678 + tcyP NIAGMN_01060 0.33 -1.9 186,678 + tcyP NIAGMN_01060 0.33 -0.3 186,678 + tcyP NIAGMN_01060 0.33 -1.8 186,679 - tcyP NIAGMN_01060 0.33 -1.7 186,814 + tcyP NIAGMN_01060 0.43 +0.9 186,814 + tcyP NIAGMN_01060 0.43 +0.8 186,814 + tcyP NIAGMN_01060 0.43 -3.5 186,866 - tcyP NIAGMN_01060 0.46 -0.3 187,020 + tcyP NIAGMN_01060 0.58 -1.5 187,037 + tcyP NIAGMN_01060 0.59 -1.5
Or see this region's nucleotide sequence