Strain Fitness in Escherichia coli ECRC102 around NIAGMN_01035

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpfkB and ydiZ are separated by 100 nucleotidesydiZ and yniA are separated by 105 nucleotides NIAGMN_01030: pfkB - 6-phosphofructokinase II, at 181,655 to 182,584 pfkB NIAGMN_01035: ydiZ - Uncharacterized protein YdiZ, at 182,685 to 182,975 ydiZ NIAGMN_01040: yniA - putative ketoamine kinase YniA, at 183,081 to 183,941 yniA Position (kb) 182 183Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 181.697 kb on - strandat 181.938 kb on + strand, within pfkBat 181.938 kb on + strand, within pfkBat 181.938 kb on + strand, within pfkBat 182.207 kb on - strand, within pfkBat 182.296 kb on + strand, within pfkBat 182.296 kb on + strand, within pfkBat 182.405 kb on - strand, within pfkBat 182.648 kb on + strandat 182.684 kb on + strandat 182.769 kb on + strand, within ydiZat 182.837 kb on + strand, within ydiZat 182.852 kb on + strand, within ydiZat 182.852 kb on + strand, within ydiZat 182.879 kb on + strand, within ydiZat 183.049 kb on + strandat 183.662 kb on - strand, within yniAat 183.700 kb on - strand, within yniA

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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181,697 - +0.6
181,938 + pfkB NIAGMN_01030 0.30 -1.8
181,938 + pfkB NIAGMN_01030 0.30 +0.7
181,938 + pfkB NIAGMN_01030 0.30 -0.3
182,207 - pfkB NIAGMN_01030 0.59 +0.2
182,296 + pfkB NIAGMN_01030 0.69 +1.6
182,296 + pfkB NIAGMN_01030 0.69 -0.6
182,405 - pfkB NIAGMN_01030 0.81 +1.9
182,648 + +0.7
182,684 + -0.2
182,769 + ydiZ NIAGMN_01035 0.29 -1.2
182,837 + ydiZ NIAGMN_01035 0.52 -0.8
182,852 + ydiZ NIAGMN_01035 0.57 +0.3
182,852 + ydiZ NIAGMN_01035 0.57 +0.9
182,879 + ydiZ NIAGMN_01035 0.67 -2.8
183,049 + -0.6
183,662 - yniA NIAGMN_01040 0.67 +0.0
183,700 - yniA NIAGMN_01040 0.72 +0.6

Or see this region's nucleotide sequence