Strain Fitness in Escherichia coli ECRC102 around NIAGMN_00640

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnemA and NIAGMN_00635 are separated by 80 nucleotidesNIAGMN_00635 and rnt are separated by 102 nucleotidesrnt and lhr are separated by 92 nucleotides NIAGMN_00630: nemA - N-ethylmaleimide reductase, at 101,956 to 103,053 nemA NIAGMN_00635: NIAGMN_00635 - Lactoylglutathione lyase, at 103,134 to 103,541 _00635 NIAGMN_00640: rnt - ribonuclease T, at 103,644 to 104,291 rnt NIAGMN_00645: lhr - ATP-dependent helicase, at 104,384 to 109,000 lhr Position (kb) 103 104 105Strain fitness (log2 ratio) -1 0 1 2 3at 102.672 kb on + strand, within nemAat 102.721 kb on + strand, within nemAat 102.765 kb on + strand, within nemAat 102.765 kb on + strand, within nemAat 102.766 kb on - strand, within nemAat 102.799 kb on + strand, within nemAat 102.865 kb on + strand, within nemAat 102.984 kb on + strandat 103.009 kb on + strandat 103.030 kb on + strandat 103.149 kb on - strandat 103.224 kb on - strand, within NIAGMN_00635at 103.308 kb on + strand, within NIAGMN_00635at 103.308 kb on + strand, within NIAGMN_00635at 103.347 kb on + strand, within NIAGMN_00635at 103.364 kb on + strand, within NIAGMN_00635at 103.373 kb on + strand, within NIAGMN_00635at 104.332 kb on - strandat 104.626 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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102,672 + nemA NIAGMN_00630 0.65 +1.1
102,721 + nemA NIAGMN_00630 0.70 -0.7
102,765 + nemA NIAGMN_00630 0.74 +1.0
102,765 + nemA NIAGMN_00630 0.74 -0.6
102,766 - nemA NIAGMN_00630 0.74 -0.6
102,799 + nemA NIAGMN_00630 0.77 -0.9
102,865 + nemA NIAGMN_00630 0.83 +0.1
102,984 + +3.1
103,009 + -1.0
103,030 + +0.3
103,149 - +0.6
103,224 - NIAGMN_00635 0.22 -0.1
103,308 + NIAGMN_00635 0.43 +3.0
103,308 + NIAGMN_00635 0.43 -0.1
103,347 + NIAGMN_00635 0.52 +0.1
103,364 + NIAGMN_00635 0.56 +0.9
103,373 + NIAGMN_00635 0.59 -0.5
104,332 - +0.5
104,626 + +0.6

Or see this region's nucleotide sequence