Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt NIAGMN_00270 and ynfA are separated by 205 nucleotides ynfA and ynfB are separated by 134 nucleotides ynfB and speG are separated by 34 nucleotides speG and ynfC are separated by 2 nucleotides
NIAGMN_00270: NIAGMN_00270 - Starvation-sensing protein, at 33,129 to 33,221
_00270
NIAGMN_00275: ynfA - YnfA family protein, at 33,427 to 33,753
ynfA
NIAGMN_00280: ynfB - DUF1283 domain-containing protein, at 33,888 to 34,229
ynfB
NIAGMN_00285: speG - spermidine N1-acetyltransferase, at 34,264 to 34,824
speG
NIAGMN_00290: ynfC - YnfC family lipoprotein, at 34,827 to 35,573
ynfC
Position (kb) 33
34
35 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 33.138 kb on - strand at 33.141 kb on - strand, within NIAGMN_00270 at 33.737 kb on - strand at 33.777 kb on + strand at 33.778 kb on - strand at 33.778 kb on - strand at 33.783 kb on + strand at 33.784 kb on - strand at 33.785 kb on + strand at 33.786 kb on - strand at 33.851 kb on + strand at 33.888 kb on - strand at 34.322 kb on + strand, within speG at 34.323 kb on - strand, within speG at 34.416 kb on + strand, within speG at 34.503 kb on - strand, within speG
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 33,138 - +1.0 33,141 - NIAGMN_00270 0.13 +0.5 33,737 - -2.8 33,777 + +0.4 33,778 - +0.1 33,778 - +0.6 33,783 + -1.1 33,784 - -0.6 33,785 + -2.9 33,786 - -0.0 33,851 + +1.9 33,888 - +1.3 34,322 + speG NIAGMN_00285 0.10 +2.9 34,323 - speG NIAGMN_00285 0.11 -3.0 34,416 + speG NIAGMN_00285 0.27 -0.1 34,503 - speG NIAGMN_00285 0.43 +3.9
Or see this region's nucleotide sequence