Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rhsA and ybfA are separated by 239 nucleotides ybfA and kdpA are separated by 401 nucleotides
MCAODC_26265: rhsA - RHS element protein, at 1,968,587 to 1,972,786
rhsA
MCAODC_26270: ybfA - Uncharacterized protein YbfA, at 1,973,026 to 1,973,232
ybfA
MCAODC_26275: kdpA - potassium-transporting ATPase subunit KdpA, at 1,973,634 to 1,975,307
kdpA
Position (kb) 1973
1974 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1972.811 kb on - strand at 1972.811 kb on - strand at 1972.904 kb on - strand at 1973.010 kb on - strand at 1973.108 kb on + strand, within ybfA at 1973.108 kb on + strand, within ybfA at 1973.109 kb on - strand, within ybfA at 1973.109 kb on - strand, within ybfA at 1973.109 kb on - strand, within ybfA at 1973.114 kb on + strand, within ybfA at 1973.180 kb on + strand, within ybfA at 1973.181 kb on - strand, within ybfA at 1973.181 kb on - strand, within ybfA at 1973.184 kb on + strand, within ybfA at 1973.185 kb on - strand, within ybfA at 1973.185 kb on - strand, within ybfA at 1973.251 kb on + strand at 1973.252 kb on - strand at 1973.255 kb on + strand at 1973.256 kb on - strand at 1973.267 kb on - strand at 1973.303 kb on + strand at 1973.366 kb on + strand at 1973.366 kb on + strand at 1973.366 kb on + strand at 1973.388 kb on - strand at 1973.525 kb on + strand at 1973.586 kb on - strand at 1973.586 kb on - strand at 1973.596 kb on + strand at 1973.605 kb on + strand at 1973.654 kb on - strand at 1973.677 kb on + strand at 1973.678 kb on - strand at 1973.680 kb on + strand at 1973.701 kb on + strand at 1973.702 kb on - strand at 1973.727 kb on - strand at 1973.732 kb on + strand at 1973.817 kb on + strand, within kdpA at 1973.817 kb on + strand, within kdpA at 1973.969 kb on + strand, within kdpA at 1973.985 kb on + strand, within kdpA at 1973.985 kb on + strand, within kdpA at 1973.986 kb on - strand, within kdpA at 1973.986 kb on - strand, within kdpA at 1973.987 kb on + strand, within kdpA at 1974.009 kb on + strand, within kdpA at 1974.010 kb on - strand, within kdpA at 1974.032 kb on - strand, within kdpA at 1974.099 kb on - strand, within kdpA at 1974.145 kb on + strand, within kdpA at 1974.146 kb on - strand, within kdpA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,972,811 - +0.6 1,972,811 - +0.8 1,972,904 - -1.2 1,973,010 - -3.1 1,973,108 + ybfA MCAODC_26270 0.40 -0.2 1,973,108 + ybfA MCAODC_26270 0.40 -0.4 1,973,109 - ybfA MCAODC_26270 0.40 +3.4 1,973,109 - ybfA MCAODC_26270 0.40 +0.0 1,973,109 - ybfA MCAODC_26270 0.40 +0.7 1,973,114 + ybfA MCAODC_26270 0.43 +0.9 1,973,180 + ybfA MCAODC_26270 0.74 -1.6 1,973,181 - ybfA MCAODC_26270 0.75 -0.0 1,973,181 - ybfA MCAODC_26270 0.75 +1.8 1,973,184 + ybfA MCAODC_26270 0.76 -0.7 1,973,185 - ybfA MCAODC_26270 0.77 -3.2 1,973,185 - ybfA MCAODC_26270 0.77 +0.3 1,973,251 + -0.1 1,973,252 - -1.2 1,973,255 + +0.6 1,973,256 - +0.0 1,973,267 - -0.5 1,973,303 + -1.2 1,973,366 + +0.2 1,973,366 + +0.2 1,973,366 + +0.7 1,973,388 - +0.8 1,973,525 + -2.0 1,973,586 - -0.1 1,973,586 - -1.3 1,973,596 + -2.5 1,973,605 + +0.8 1,973,654 - +0.2 1,973,677 + -2.0 1,973,678 - -0.9 1,973,680 + +0.4 1,973,701 + +0.2 1,973,702 - -0.5 1,973,727 - -1.0 1,973,732 + +0.1 1,973,817 + kdpA MCAODC_26275 0.11 +0.5 1,973,817 + kdpA MCAODC_26275 0.11 +0.3 1,973,969 + kdpA MCAODC_26275 0.20 +0.3 1,973,985 + kdpA MCAODC_26275 0.21 +0.2 1,973,985 + kdpA MCAODC_26275 0.21 +2.7 1,973,986 - kdpA MCAODC_26275 0.21 -1.1 1,973,986 - kdpA MCAODC_26275 0.21 +0.6 1,973,987 + kdpA MCAODC_26275 0.21 -1.3 1,974,009 + kdpA MCAODC_26275 0.22 +0.6 1,974,010 - kdpA MCAODC_26275 0.22 -0.2 1,974,032 - kdpA MCAODC_26275 0.24 +0.4 1,974,099 - kdpA MCAODC_26275 0.28 +0.6 1,974,145 + kdpA MCAODC_26275 0.31 +0.8 1,974,146 - kdpA MCAODC_26275 0.31 +1.3
Or see this region's nucleotide sequence