Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hcp and hcr are separated by 11 nucleotides hcr and poxB are separated by 132 nucleotides
MCAODC_25050: hcp - hydroxylamine reductase, at 1,735,786 to 1,737,438
hcp
MCAODC_25055: hcr - NADH oxidoreductase, at 1,737,450 to 1,738,418
hcr
MCAODC_25060: poxB - ubiquinone-dependent pyruvate dehydrogenase, at 1,738,551 to 1,740,269
poxB
Position (kb)
1737
1738
1739 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 1736.457 kb on + strand, within hcp at 1736.461 kb on + strand, within hcp at 1736.462 kb on - strand, within hcp at 1736.462 kb on - strand, within hcp at 1736.590 kb on + strand, within hcp at 1736.590 kb on + strand, within hcp at 1736.637 kb on + strand, within hcp at 1736.663 kb on - strand, within hcp at 1736.761 kb on + strand, within hcp at 1736.773 kb on - strand, within hcp at 1736.773 kb on - strand, within hcp at 1736.940 kb on + strand, within hcp at 1736.945 kb on + strand, within hcp at 1736.946 kb on - strand, within hcp at 1736.979 kb on + strand, within hcp at 1736.980 kb on - strand, within hcp at 1736.980 kb on - strand, within hcp at 1736.980 kb on - strand, within hcp at 1737.088 kb on - strand, within hcp at 1737.159 kb on + strand, within hcp at 1737.169 kb on + strand, within hcp at 1737.169 kb on + strand, within hcp at 1737.394 kb on - strand at 1737.501 kb on - strand at 1737.501 kb on - strand at 1737.501 kb on - strand at 1737.549 kb on + strand, within hcr at 1737.549 kb on + strand, within hcr at 1737.550 kb on - strand, within hcr at 1737.553 kb on - strand, within hcr at 1737.573 kb on + strand, within hcr at 1737.574 kb on - strand, within hcr at 1737.593 kb on + strand, within hcr at 1737.618 kb on + strand, within hcr at 1737.618 kb on + strand, within hcr at 1737.619 kb on - strand, within hcr at 1737.651 kb on + strand, within hcr at 1737.651 kb on + strand, within hcr at 1737.651 kb on + strand, within hcr at 1737.651 kb on + strand, within hcr at 1737.652 kb on - strand, within hcr at 1737.652 kb on - strand, within hcr at 1737.653 kb on + strand, within hcr at 1737.653 kb on + strand, within hcr at 1737.654 kb on - strand, within hcr at 1737.654 kb on - strand, within hcr at 1737.729 kb on + strand, within hcr at 1737.729 kb on + strand, within hcr at 1737.729 kb on + strand, within hcr at 1737.729 kb on + strand, within hcr at 1737.729 kb on + strand, within hcr at 1737.730 kb on - strand, within hcr at 1737.730 kb on - strand, within hcr at 1737.774 kb on - strand, within hcr at 1737.774 kb on - strand, within hcr at 1737.828 kb on - strand, within hcr at 1737.885 kb on + strand, within hcr at 1737.885 kb on + strand, within hcr at 1737.885 kb on + strand, within hcr at 1737.885 kb on + strand, within hcr at 1737.886 kb on - strand, within hcr at 1737.896 kb on + strand, within hcr at 1737.932 kb on + strand, within hcr at 1737.933 kb on - strand, within hcr at 1737.962 kb on + strand, within hcr at 1737.980 kb on + strand, within hcr at 1738.027 kb on + strand, within hcr at 1738.027 kb on + strand, within hcr at 1738.028 kb on - strand, within hcr at 1738.028 kb on - strand, within hcr at 1738.028 kb on - strand, within hcr at 1738.066 kb on - strand, within hcr at 1738.066 kb on - strand, within hcr at 1738.066 kb on - strand, within hcr at 1738.067 kb on + strand, within hcr at 1738.319 kb on + strand, within hcr at 1738.319 kb on + strand, within hcr at 1738.320 kb on - strand, within hcr at 1738.331 kb on + strand at 1738.331 kb on + strand at 1738.368 kb on + strand at 1738.369 kb on - strand at 1738.373 kb on - strand at 1738.373 kb on - strand at 1738.426 kb on - strand at 1738.455 kb on - strand at 1738.511 kb on + strand at 1738.511 kb on + strand at 1738.511 kb on + strand at 1738.512 kb on - strand at 1738.512 kb on - strand at 1738.572 kb on + strand at 1738.572 kb on + strand at 1738.572 kb on + strand at 1738.572 kb on + strand at 1738.573 kb on - strand at 1738.573 kb on - strand at 1738.573 kb on - strand at 1738.573 kb on - strand at 1738.646 kb on + strand at 1738.658 kb on + strand at 1738.659 kb on - strand at 1738.792 kb on + strand, within poxB at 1738.792 kb on + strand, within poxB at 1738.792 kb on + strand, within poxB at 1738.793 kb on - strand, within poxB at 1738.793 kb on - strand, within poxB at 1738.793 kb on - strand, within poxB at 1738.793 kb on - strand, within poxB at 1738.909 kb on + strand, within poxB at 1738.910 kb on - strand, within poxB at 1738.922 kb on + strand, within poxB at 1738.922 kb on + strand, within poxB at 1739.001 kb on - strand, within poxB at 1739.069 kb on + strand, within poxB at 1739.080 kb on - strand, within poxB at 1739.122 kb on + strand, within poxB at 1739.123 kb on - strand, within poxB at 1739.237 kb on + strand, within poxB at 1739.238 kb on - strand, within poxB at 1739.238 kb on - strand, within poxB at 1739.238 kb on - strand, within poxB at 1739.238 kb on - strand, within poxB at 1739.238 kb on - strand, within poxB at 1739.238 kb on - strand, within poxB at 1739.258 kb on + strand, within poxB at 1739.286 kb on + strand, within poxB at 1739.286 kb on + strand, within poxB at 1739.362 kb on + strand, within poxB at 1739.362 kb on + strand, within poxB at 1739.363 kb on - strand, within poxB
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,736,457 + hcp MCAODC_25050 0.41 +0.9 1,736,461 + hcp MCAODC_25050 0.41 -1.4 1,736,462 - hcp MCAODC_25050 0.41 +0.0 1,736,462 - hcp MCAODC_25050 0.41 -1.5 1,736,590 + hcp MCAODC_25050 0.49 +0.5 1,736,590 + hcp MCAODC_25050 0.49 -0.6 1,736,637 + hcp MCAODC_25050 0.51 -0.5 1,736,663 - hcp MCAODC_25050 0.53 +1.0 1,736,761 + hcp MCAODC_25050 0.59 +0.6 1,736,773 - hcp MCAODC_25050 0.60 -2.1 1,736,773 - hcp MCAODC_25050 0.60 +0.1 1,736,940 + hcp MCAODC_25050 0.70 -1.3 1,736,945 + hcp MCAODC_25050 0.70 +0.0 1,736,946 - hcp MCAODC_25050 0.70 +1.0 1,736,979 + hcp MCAODC_25050 0.72 -0.3 1,736,980 - hcp MCAODC_25050 0.72 +1.1 1,736,980 - hcp MCAODC_25050 0.72 +0.5 1,736,980 - hcp MCAODC_25050 0.72 -1.8 1,737,088 - hcp MCAODC_25050 0.79 +1.4 1,737,159 + hcp MCAODC_25050 0.83 +0.0 1,737,169 + hcp MCAODC_25050 0.84 -0.2 1,737,169 + hcp MCAODC_25050 0.84 -1.9 1,737,394 - -0.5 1,737,501 - +0.8 1,737,501 - +2.9 1,737,501 - +2.1 1,737,549 + hcr MCAODC_25055 0.10 -1.8 1,737,549 + hcr MCAODC_25055 0.10 +0.4 1,737,550 - hcr MCAODC_25055 0.10 -1.9 1,737,553 - hcr MCAODC_25055 0.11 -0.3 1,737,573 + hcr MCAODC_25055 0.13 -0.5 1,737,574 - hcr MCAODC_25055 0.13 +1.0 1,737,593 + hcr MCAODC_25055 0.15 +0.3 1,737,618 + hcr MCAODC_25055 0.17 +0.1 1,737,618 + hcr MCAODC_25055 0.17 +0.1 1,737,619 - hcr MCAODC_25055 0.17 +0.7 1,737,651 + hcr MCAODC_25055 0.21 -0.5 1,737,651 + hcr MCAODC_25055 0.21 +0.2 1,737,651 + hcr MCAODC_25055 0.21 -2.0 1,737,651 + hcr MCAODC_25055 0.21 +0.7 1,737,652 - hcr MCAODC_25055 0.21 -1.2 1,737,652 - hcr MCAODC_25055 0.21 -0.4 1,737,653 + hcr MCAODC_25055 0.21 +0.6 1,737,653 + hcr MCAODC_25055 0.21 +0.9 1,737,654 - hcr MCAODC_25055 0.21 -3.1 1,737,654 - hcr MCAODC_25055 0.21 -0.4 1,737,729 + hcr MCAODC_25055 0.29 -2.7 1,737,729 + hcr MCAODC_25055 0.29 -2.1 1,737,729 + hcr MCAODC_25055 0.29 +1.5 1,737,729 + hcr MCAODC_25055 0.29 +0.5 1,737,729 + hcr MCAODC_25055 0.29 +0.1 1,737,730 - hcr MCAODC_25055 0.29 -2.9 1,737,730 - hcr MCAODC_25055 0.29 -0.3 1,737,774 - hcr MCAODC_25055 0.33 -0.7 1,737,774 - hcr MCAODC_25055 0.33 -0.4 1,737,828 - hcr MCAODC_25055 0.39 -2.4 1,737,885 + hcr MCAODC_25055 0.45 -0.4 1,737,885 + hcr MCAODC_25055 0.45 +1.2 1,737,885 + hcr MCAODC_25055 0.45 -0.3 1,737,885 + hcr MCAODC_25055 0.45 +1.2 1,737,886 - hcr MCAODC_25055 0.45 +1.2 1,737,896 + hcr MCAODC_25055 0.46 -1.4 1,737,932 + hcr MCAODC_25055 0.50 +0.2 1,737,933 - hcr MCAODC_25055 0.50 -2.9 1,737,962 + hcr MCAODC_25055 0.53 -1.2 1,737,980 + hcr MCAODC_25055 0.55 +0.5 1,738,027 + hcr MCAODC_25055 0.60 +0.6 1,738,027 + hcr MCAODC_25055 0.60 +1.3 1,738,028 - hcr MCAODC_25055 0.60 -1.1 1,738,028 - hcr MCAODC_25055 0.60 -0.3 1,738,028 - hcr MCAODC_25055 0.60 -0.6 1,738,066 - hcr MCAODC_25055 0.64 -2.9 1,738,066 - hcr MCAODC_25055 0.64 +0.4 1,738,066 - hcr MCAODC_25055 0.64 +1.4 1,738,067 + hcr MCAODC_25055 0.64 -2.9 1,738,319 + hcr MCAODC_25055 0.90 +0.3 1,738,319 + hcr MCAODC_25055 0.90 -3.1 1,738,320 - hcr MCAODC_25055 0.90 -0.8 1,738,331 + +1.3 1,738,331 + +1.2 1,738,368 + +0.5 1,738,369 - +0.3 1,738,373 - -0.2 1,738,373 - +0.3 1,738,426 - -0.7 1,738,455 - +1.3 1,738,511 + +1.2 1,738,511 + -0.3 1,738,511 + +0.8 1,738,512 - +2.0 1,738,512 - -0.3 1,738,572 + +0.2 1,738,572 + -0.0 1,738,572 + +2.2 1,738,572 + +0.6 1,738,573 - -0.3 1,738,573 - -2.0 1,738,573 - -0.5 1,738,573 - -0.6 1,738,646 + -0.9 1,738,658 + -3.1 1,738,659 - +0.7 1,738,792 + poxB MCAODC_25060 0.14 -2.2 1,738,792 + poxB MCAODC_25060 0.14 -5.6 1,738,792 + poxB MCAODC_25060 0.14 +0.4 1,738,793 - poxB MCAODC_25060 0.14 -0.4 1,738,793 - poxB MCAODC_25060 0.14 -0.9 1,738,793 - poxB MCAODC_25060 0.14 +0.6 1,738,793 - poxB MCAODC_25060 0.14 -1.7 1,738,909 + poxB MCAODC_25060 0.21 -3.2 1,738,910 - poxB MCAODC_25060 0.21 +0.5 1,738,922 + poxB MCAODC_25060 0.22 -2.3 1,738,922 + poxB MCAODC_25060 0.22 +0.9 1,739,001 - poxB MCAODC_25060 0.26 +1.4 1,739,069 + poxB MCAODC_25060 0.30 -0.4 1,739,080 - poxB MCAODC_25060 0.31 +0.1 1,739,122 + poxB MCAODC_25060 0.33 -2.1 1,739,123 - poxB MCAODC_25060 0.33 -0.1 1,739,237 + poxB MCAODC_25060 0.40 -1.1 1,739,238 - poxB MCAODC_25060 0.40 +0.7 1,739,238 - poxB MCAODC_25060 0.40 -0.3 1,739,238 - poxB MCAODC_25060 0.40 -1.0 1,739,238 - poxB MCAODC_25060 0.40 +0.3 1,739,238 - poxB MCAODC_25060 0.40 +1.5 1,739,238 - poxB MCAODC_25060 0.40 -0.8 1,739,258 + poxB MCAODC_25060 0.41 -0.7 1,739,286 + poxB MCAODC_25060 0.43 +0.5 1,739,286 + poxB MCAODC_25060 0.43 -0.3 1,739,362 + poxB MCAODC_25060 0.47 -0.6 1,739,362 + poxB MCAODC_25060 0.47 +0.9 1,739,363 - poxB MCAODC_25060 0.47 +0.4
Or see this region's nucleotide sequence