Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rmf and pqiC are separated by 255 nucleotides pqiC and pqiB overlap by 4 nucleotides pqiB and pqiA are separated by 4 nucleotides
MCAODC_24645: rmf - ribosome modulation factor, at 1,635,359 to 1,635,526
rmf
MCAODC_24650: pqiC - membrane integrity-associated transporter subunit PqiC, at 1,635,782 to 1,636,345
pqiC
MCAODC_24655: pqiB - intermembrane transport protein PqiB, at 1,636,342 to 1,637,982
pqiB
MCAODC_24660: pqiA - membrane integrity-associated transporter subunit PqiA, at 1,637,987 to 1,639,240
pqiA
Position (kb)
1636
1637
1638 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1635.368 kb on + strand at 1635.369 kb on - strand at 1635.369 kb on - strand at 1635.543 kb on - strand at 1635.575 kb on - strand at 1635.596 kb on - strand at 1635.596 kb on - strand at 1635.602 kb on + strand at 1635.652 kb on - strand at 1635.652 kb on - strand at 1635.720 kb on - strand at 1635.788 kb on + strand at 1635.789 kb on - strand at 1635.798 kb on - strand at 1635.812 kb on - strand at 1635.812 kb on - strand at 1635.976 kb on + strand, within pqiC at 1636.135 kb on - strand, within pqiC at 1636.135 kb on - strand, within pqiC at 1636.180 kb on - strand, within pqiC at 1636.203 kb on - strand, within pqiC at 1636.209 kb on - strand, within pqiC at 1636.263 kb on - strand, within pqiC at 1636.273 kb on - strand, within pqiC at 1636.470 kb on + strand at 1636.491 kb on - strand at 1636.705 kb on + strand, within pqiB at 1636.705 kb on - strand, within pqiB at 1636.705 kb on - strand, within pqiB at 1636.780 kb on - strand, within pqiB at 1636.836 kb on - strand, within pqiB at 1636.836 kb on - strand, within pqiB at 1636.836 kb on - strand, within pqiB at 1636.836 kb on - strand, within pqiB at 1636.981 kb on - strand, within pqiB at 1636.986 kb on - strand, within pqiB at 1637.172 kb on - strand, within pqiB at 1637.320 kb on - strand, within pqiB at 1637.361 kb on - strand, within pqiB at 1637.385 kb on - strand, within pqiB at 1637.563 kb on - strand, within pqiB at 1637.604 kb on - strand, within pqiB at 1637.604 kb on - strand, within pqiB at 1637.604 kb on - strand, within pqiB at 1637.686 kb on - strand, within pqiB at 1637.797 kb on - strand, within pqiB at 1637.870 kb on + strand at 1637.871 kb on - strand at 1637.971 kb on - strand at 1637.974 kb on - strand at 1638.113 kb on - strand, within pqiA at 1638.120 kb on - strand, within pqiA at 1638.301 kb on + strand, within pqiA at 1638.304 kb on + strand, within pqiA at 1638.305 kb on - strand, within pqiA at 1638.402 kb on - strand, within pqiA at 1638.428 kb on - strand, within pqiA at 1638.457 kb on + strand, within pqiA at 1638.458 kb on - strand, within pqiA at 1638.458 kb on - strand, within pqiA at 1638.511 kb on + strand, within pqiA at 1638.512 kb on - strand, within pqiA at 1638.515 kb on - strand, within pqiA at 1638.515 kb on - strand, within pqiA at 1638.611 kb on + strand, within pqiA at 1638.848 kb on + strand, within pqiA at 1638.848 kb on + strand, within pqiA at 1638.882 kb on - strand, within pqiA at 1638.954 kb on + strand, within pqiA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,635,368 + +0.6 1,635,369 - -0.4 1,635,369 - -0.8 1,635,543 - -2.3 1,635,575 - +0.8 1,635,596 - +1.5 1,635,596 - -0.4 1,635,602 + -1.3 1,635,652 - -0.9 1,635,652 - -0.8 1,635,720 - +0.3 1,635,788 + +0.5 1,635,789 - +0.2 1,635,798 - -0.9 1,635,812 - -0.0 1,635,812 - -2.1 1,635,976 + pqiC MCAODC_24650 0.34 -1.6 1,636,135 - pqiC MCAODC_24650 0.63 +1.1 1,636,135 - pqiC MCAODC_24650 0.63 +0.2 1,636,180 - pqiC MCAODC_24650 0.71 -0.6 1,636,203 - pqiC MCAODC_24650 0.75 +1.6 1,636,209 - pqiC MCAODC_24650 0.76 +0.2 1,636,263 - pqiC MCAODC_24650 0.85 -0.3 1,636,273 - pqiC MCAODC_24650 0.87 +1.4 1,636,470 + +0.7 1,636,491 - +0.1 1,636,705 + pqiB MCAODC_24655 0.22 -0.8 1,636,705 - pqiB MCAODC_24655 0.22 +1.0 1,636,705 - pqiB MCAODC_24655 0.22 -0.7 1,636,780 - pqiB MCAODC_24655 0.27 -3.4 1,636,836 - pqiB MCAODC_24655 0.30 +2.4 1,636,836 - pqiB MCAODC_24655 0.30 +0.5 1,636,836 - pqiB MCAODC_24655 0.30 +0.5 1,636,836 - pqiB MCAODC_24655 0.30 +1.2 1,636,981 - pqiB MCAODC_24655 0.39 -0.3 1,636,986 - pqiB MCAODC_24655 0.39 +0.0 1,637,172 - pqiB MCAODC_24655 0.51 -0.8 1,637,320 - pqiB MCAODC_24655 0.60 +0.7 1,637,361 - pqiB MCAODC_24655 0.62 -0.1 1,637,385 - pqiB MCAODC_24655 0.64 -2.6 1,637,563 - pqiB MCAODC_24655 0.74 +0.3 1,637,604 - pqiB MCAODC_24655 0.77 -0.6 1,637,604 - pqiB MCAODC_24655 0.77 -0.8 1,637,604 - pqiB MCAODC_24655 0.77 -3.8 1,637,686 - pqiB MCAODC_24655 0.82 -0.3 1,637,797 - pqiB MCAODC_24655 0.89 -1.3 1,637,870 + +1.6 1,637,871 - -2.0 1,637,971 - +0.3 1,637,974 - -1.1 1,638,113 - pqiA MCAODC_24660 0.10 +0.3 1,638,120 - pqiA MCAODC_24660 0.11 +0.8 1,638,301 + pqiA MCAODC_24660 0.25 +0.5 1,638,304 + pqiA MCAODC_24660 0.25 -1.3 1,638,305 - pqiA MCAODC_24660 0.25 -0.6 1,638,402 - pqiA MCAODC_24660 0.33 +0.2 1,638,428 - pqiA MCAODC_24660 0.35 +0.3 1,638,457 + pqiA MCAODC_24660 0.37 +2.8 1,638,458 - pqiA MCAODC_24660 0.38 -0.1 1,638,458 - pqiA MCAODC_24660 0.38 +0.4 1,638,511 + pqiA MCAODC_24660 0.42 +0.5 1,638,512 - pqiA MCAODC_24660 0.42 +0.3 1,638,515 - pqiA MCAODC_24660 0.42 +0.2 1,638,515 - pqiA MCAODC_24660 0.42 -1.0 1,638,611 + pqiA MCAODC_24660 0.50 -1.7 1,638,848 + pqiA MCAODC_24660 0.69 -2.1 1,638,848 + pqiA MCAODC_24660 0.69 +0.4 1,638,882 - pqiA MCAODC_24660 0.71 -1.6 1,638,954 + pqiA MCAODC_24660 0.77 -0.2
Or see this region's nucleotide sequence