Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MCAODC_23415 and tnp-IS1 are separated by 128 nucleotides tnp-IS1 and yjiC are separated by 418 nucleotides yjiC and alpA are separated by 729 nucleotides
MCAODC_23415: MCAODC_23415 - HTH-Tnp-IS1 domain-containing protein, at 1,412,296 to 1,412,355
_23415
MCAODC_23420: tnp-IS1 - IS1 family transposase, at 1,412,484 to 1,412,777
tnp-IS1
MCAODC_23425: yjiC - glucosyl transferase, at 1,413,196 to 1,414,320
yjiC
MCAODC_23430: alpA - AlpA family transcriptional regulator, at 1,415,050 to 1,415,247
alpA
Position (kb) 1413
1414
1415 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 1412.202 kb on - strand at 1412.202 kb on - strand at 1412.331 kb on - strand, within MCAODC_23415 at 1412.365 kb on + strand at 1412.419 kb on - strand at 1412.455 kb on - strand at 1412.564 kb on - strand, within tnp-IS1 at 1412.590 kb on - strand, within tnp-IS1 at 1412.751 kb on + strand at 1412.761 kb on + strand at 1412.762 kb on - strand at 1412.769 kb on + strand at 1412.800 kb on - strand at 1413.193 kb on - strand at 1413.452 kb on + strand, within yjiC at 1413.471 kb on + strand, within yjiC at 1413.472 kb on - strand, within yjiC at 1413.525 kb on + strand, within yjiC at 1413.530 kb on - strand, within yjiC at 1413.607 kb on - strand, within yjiC at 1413.693 kb on + strand, within yjiC at 1413.694 kb on - strand, within yjiC at 1413.694 kb on - strand, within yjiC at 1413.698 kb on - strand, within yjiC at 1413.763 kb on - strand, within yjiC at 1413.765 kb on - strand, within yjiC at 1413.811 kb on - strand, within yjiC at 1413.839 kb on - strand, within yjiC at 1413.848 kb on - strand, within yjiC at 1413.876 kb on - strand, within yjiC at 1413.876 kb on - strand, within yjiC at 1413.910 kb on - strand, within yjiC at 1413.922 kb on - strand, within yjiC at 1413.922 kb on - strand, within yjiC at 1413.963 kb on - strand, within yjiC at 1413.967 kb on - strand, within yjiC at 1414.009 kb on + strand, within yjiC at 1414.223 kb on - strand at 1414.529 kb on + strand at 1414.614 kb on + strand at 1414.806 kb on + strand at 1414.819 kb on - strand at 1414.825 kb on + strand at 1414.985 kb on - strand at 1414.985 kb on - strand at 1415.033 kb on - strand at 1415.033 kb on - strand at 1415.078 kb on + strand, within alpA at 1415.112 kb on + strand, within alpA at 1415.160 kb on + strand, within alpA at 1415.178 kb on + strand, within alpA at 1415.179 kb on - strand, within alpA at 1415.180 kb on + strand, within alpA at 1415.180 kb on + strand, within alpA at 1415.180 kb on + strand, within alpA at 1415.180 kb on + strand, within alpA at 1415.181 kb on - strand, within alpA at 1415.181 kb on - strand, within alpA at 1415.218 kb on + strand, within alpA at 1415.219 kb on - strand, within alpA at 1415.238 kb on + strand at 1415.238 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,412,202 - +1.1 1,412,202 - +2.7 1,412,331 - MCAODC_23415 0.58 +1.1 1,412,365 + -2.8 1,412,419 - +1.0 1,412,455 - +2.7 1,412,564 - tnp-IS1 MCAODC_23420 0.27 +0.5 1,412,590 - tnp-IS1 MCAODC_23420 0.36 +0.8 1,412,751 + +0.3 1,412,761 + -1.8 1,412,762 - +1.7 1,412,769 + +0.3 1,412,800 - -1.6 1,413,193 - +1.4 1,413,452 + yjiC MCAODC_23425 0.23 +0.8 1,413,471 + yjiC MCAODC_23425 0.24 -0.2 1,413,472 - yjiC MCAODC_23425 0.25 -0.6 1,413,525 + yjiC MCAODC_23425 0.29 +0.3 1,413,530 - yjiC MCAODC_23425 0.30 -1.7 1,413,607 - yjiC MCAODC_23425 0.37 +0.4 1,413,693 + yjiC MCAODC_23425 0.44 -1.1 1,413,694 - yjiC MCAODC_23425 0.44 -0.3 1,413,694 - yjiC MCAODC_23425 0.44 -0.1 1,413,698 - yjiC MCAODC_23425 0.45 +1.1 1,413,763 - yjiC MCAODC_23425 0.50 +1.1 1,413,765 - yjiC MCAODC_23425 0.51 -1.4 1,413,811 - yjiC MCAODC_23425 0.55 -0.7 1,413,839 - yjiC MCAODC_23425 0.57 +0.9 1,413,848 - yjiC MCAODC_23425 0.58 -0.6 1,413,876 - yjiC MCAODC_23425 0.60 +0.9 1,413,876 - yjiC MCAODC_23425 0.60 -2.1 1,413,910 - yjiC MCAODC_23425 0.63 +1.3 1,413,922 - yjiC MCAODC_23425 0.65 +0.4 1,413,922 - yjiC MCAODC_23425 0.65 +0.4 1,413,963 - yjiC MCAODC_23425 0.68 -0.4 1,413,967 - yjiC MCAODC_23425 0.69 +0.3 1,414,009 + yjiC MCAODC_23425 0.72 +0.6 1,414,223 - +1.4 1,414,529 + -0.0 1,414,614 + +1.2 1,414,806 + +3.0 1,414,819 - +0.4 1,414,825 + +0.7 1,414,985 - +0.4 1,414,985 - +0.6 1,415,033 - +0.4 1,415,033 - +0.4 1,415,078 + alpA MCAODC_23430 0.14 +0.6 1,415,112 + alpA MCAODC_23430 0.31 -0.8 1,415,160 + alpA MCAODC_23430 0.56 +0.6 1,415,178 + alpA MCAODC_23430 0.65 +1.0 1,415,179 - alpA MCAODC_23430 0.65 +0.9 1,415,180 + alpA MCAODC_23430 0.66 +0.6 1,415,180 + alpA MCAODC_23430 0.66 -0.1 1,415,180 + alpA MCAODC_23430 0.66 +0.1 1,415,180 + alpA MCAODC_23430 0.66 -0.7 1,415,181 - alpA MCAODC_23430 0.66 -1.0 1,415,181 - alpA MCAODC_23430 0.66 +0.2 1,415,218 + alpA MCAODC_23430 0.85 -1.5 1,415,219 - alpA MCAODC_23430 0.85 -0.0 1,415,238 + -0.6 1,415,238 + -0.8
Or see this region's nucleotide sequence