Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt grxB and yceB are separated by 133 nucleotides yceB and pyrC are separated by 105 nucleotides pyrC and dinI are separated by 73 nucleotides dinI and bssS are separated by 289 nucleotides bssS and solA are separated by 114 nucleotides
MCAODC_23080: grxB - glutaredoxin 2, at 1,357,281 to 1,357,928
grxB
MCAODC_23085: yceB - lipoprotein, at 1,358,062 to 1,358,622
yceB
MCAODC_23090: pyrC - dihydroorotase, at 1,358,728 to 1,359,774
pyrC
MCAODC_23095: dinI - DNA damage-inducible protein I, at 1,359,848 to 1,360,093
dinI
MCAODC_23100: bssS - biofilm formation regulator BssS, at 1,360,383 to 1,360,637
bssS
MCAODC_23105: solA - N-methyl-L-tryptophan oxidase, at 1,360,752 to 1,361,870
solA
Position (kb)
1358
1359
1360 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 1357.732 kb on + strand, within grxB at 1357.732 kb on + strand, within grxB at 1357.796 kb on + strand, within grxB at 1357.797 kb on - strand, within grxB at 1357.804 kb on + strand, within grxB at 1357.844 kb on + strand, within grxB at 1357.844 kb on + strand, within grxB at 1357.909 kb on + strand at 1357.997 kb on + strand at 1358.075 kb on + strand at 1358.076 kb on - strand at 1358.134 kb on + strand, within yceB at 1358.134 kb on + strand, within yceB at 1358.134 kb on + strand, within yceB at 1358.135 kb on - strand, within yceB at 1358.157 kb on + strand, within yceB at 1358.178 kb on + strand, within yceB at 1358.196 kb on + strand, within yceB at 1358.196 kb on + strand, within yceB at 1358.226 kb on + strand, within yceB at 1358.429 kb on + strand, within yceB at 1358.473 kb on + strand, within yceB at 1358.489 kb on + strand, within yceB at 1358.489 kb on + strand, within yceB at 1358.620 kb on + strand at 1358.720 kb on - strand at 1358.720 kb on - strand at 1358.726 kb on + strand at 1358.727 kb on - strand at 1358.748 kb on - strand at 1358.853 kb on - strand, within pyrC at 1358.965 kb on + strand, within pyrC at 1358.965 kb on + strand, within pyrC at 1359.200 kb on + strand, within pyrC at 1359.200 kb on + strand, within pyrC at 1359.201 kb on - strand, within pyrC at 1359.286 kb on + strand, within pyrC at 1359.287 kb on - strand, within pyrC at 1359.357 kb on + strand, within pyrC at 1359.357 kb on + strand, within pyrC at 1359.357 kb on + strand, within pyrC at 1359.358 kb on - strand, within pyrC at 1359.388 kb on + strand, within pyrC at 1359.389 kb on - strand, within pyrC at 1359.562 kb on + strand, within pyrC at 1359.637 kb on + strand, within pyrC at 1359.637 kb on + strand, within pyrC at 1359.752 kb on + strand at 1359.753 kb on - strand at 1359.798 kb on + strand at 1359.798 kb on + strand at 1359.798 kb on + strand at 1359.799 kb on - strand at 1359.799 kb on - strand at 1359.799 kb on - strand at 1359.844 kb on + strand at 1359.847 kb on + strand at 1359.938 kb on + strand, within dinI at 1359.966 kb on + strand, within dinI at 1359.967 kb on - strand, within dinI at 1359.972 kb on + strand, within dinI at 1359.973 kb on - strand, within dinI at 1359.991 kb on + strand, within dinI at 1360.094 kb on + strand at 1360.300 kb on + strand at 1360.301 kb on - strand at 1360.309 kb on - strand at 1360.358 kb on + strand at 1360.423 kb on + strand, within bssS at 1360.448 kb on + strand, within bssS at 1360.452 kb on + strand, within bssS at 1360.494 kb on - strand, within bssS at 1360.573 kb on + strand, within bssS at 1360.573 kb on + strand, within bssS at 1360.697 kb on + strand at 1360.697 kb on + strand at 1360.698 kb on - strand at 1360.698 kb on - strand at 1360.698 kb on - strand at 1360.699 kb on + strand at 1360.699 kb on + strand at 1360.699 kb on + strand at 1360.717 kb on + strand at 1360.718 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,357,732 + grxB MCAODC_23080 0.70 +0.3 1,357,732 + grxB MCAODC_23080 0.70 +0.3 1,357,796 + grxB MCAODC_23080 0.79 +1.9 1,357,797 - grxB MCAODC_23080 0.80 -0.5 1,357,804 + grxB MCAODC_23080 0.81 -0.2 1,357,844 + grxB MCAODC_23080 0.87 -0.2 1,357,844 + grxB MCAODC_23080 0.87 +0.0 1,357,909 + +1.1 1,357,997 + +0.0 1,358,075 + +0.1 1,358,076 - -0.2 1,358,134 + yceB MCAODC_23085 0.13 -0.2 1,358,134 + yceB MCAODC_23085 0.13 +0.8 1,358,134 + yceB MCAODC_23085 0.13 +2.3 1,358,135 - yceB MCAODC_23085 0.13 -2.0 1,358,157 + yceB MCAODC_23085 0.17 -2.6 1,358,178 + yceB MCAODC_23085 0.21 +0.1 1,358,196 + yceB MCAODC_23085 0.24 -1.3 1,358,196 + yceB MCAODC_23085 0.24 -0.2 1,358,226 + yceB MCAODC_23085 0.29 +0.1 1,358,429 + yceB MCAODC_23085 0.65 -1.1 1,358,473 + yceB MCAODC_23085 0.73 +0.9 1,358,489 + yceB MCAODC_23085 0.76 +0.5 1,358,489 + yceB MCAODC_23085 0.76 -2.5 1,358,620 + -1.2 1,358,720 - -1.8 1,358,720 - -3.1 1,358,726 + -1.5 1,358,727 - -3.6 1,358,748 - -5.3 1,358,853 - pyrC MCAODC_23090 0.12 -3.6 1,358,965 + pyrC MCAODC_23090 0.23 -3.5 1,358,965 + pyrC MCAODC_23090 0.23 -1.8 1,359,200 + pyrC MCAODC_23090 0.45 -3.7 1,359,200 + pyrC MCAODC_23090 0.45 -1.5 1,359,201 - pyrC MCAODC_23090 0.45 -3.9 1,359,286 + pyrC MCAODC_23090 0.53 -3.3 1,359,287 - pyrC MCAODC_23090 0.53 -3.5 1,359,357 + pyrC MCAODC_23090 0.60 -2.2 1,359,357 + pyrC MCAODC_23090 0.60 -3.1 1,359,357 + pyrC MCAODC_23090 0.60 +0.7 1,359,358 - pyrC MCAODC_23090 0.60 -1.9 1,359,388 + pyrC MCAODC_23090 0.63 +1.7 1,359,389 - pyrC MCAODC_23090 0.63 -2.6 1,359,562 + pyrC MCAODC_23090 0.80 -0.6 1,359,637 + pyrC MCAODC_23090 0.87 -5.0 1,359,637 + pyrC MCAODC_23090 0.87 -6.7 1,359,752 + -0.1 1,359,753 - -3.1 1,359,798 + +0.2 1,359,798 + +0.7 1,359,798 + -2.9 1,359,799 - +0.1 1,359,799 - +2.7 1,359,799 - +1.1 1,359,844 + +0.8 1,359,847 + +1.5 1,359,938 + dinI MCAODC_23095 0.37 -0.1 1,359,966 + dinI MCAODC_23095 0.48 +0.2 1,359,967 - dinI MCAODC_23095 0.48 -2.0 1,359,972 + dinI MCAODC_23095 0.50 -0.4 1,359,973 - dinI MCAODC_23095 0.51 +0.7 1,359,991 + dinI MCAODC_23095 0.58 -0.3 1,360,094 + +0.4 1,360,300 + +0.6 1,360,301 - +1.7 1,360,309 - +0.9 1,360,358 + +1.2 1,360,423 + bssS MCAODC_23100 0.16 -1.5 1,360,448 + bssS MCAODC_23100 0.25 -0.0 1,360,452 + bssS MCAODC_23100 0.27 +0.2 1,360,494 - bssS MCAODC_23100 0.44 +1.9 1,360,573 + bssS MCAODC_23100 0.75 +0.4 1,360,573 + bssS MCAODC_23100 0.75 +0.2 1,360,697 + -0.6 1,360,697 + -1.3 1,360,698 - +1.4 1,360,698 - +1.7 1,360,698 - +2.5 1,360,699 + +1.1 1,360,699 + -2.9 1,360,699 + -0.1 1,360,717 + -0.9 1,360,718 - +0.6
Or see this region's nucleotide sequence