Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt espK and MCAODC_22475 are separated by 188 nucleotides MCAODC_22475 and MCAODC_22480 are separated by 141 nucleotides MCAODC_22480 and tnp-IS3 are separated by 10 nucleotides tnp-IS3 and MCAODC_22490 are separated by 161 nucleotides
MCAODC_22470: espK - type III secretion system effector EspK, at 1,253,637 to 1,254,998
espK
MCAODC_22475: MCAODC_22475 - hypothetical protein, at 1,255,187 to 1,255,420
_22475
MCAODC_22480: MCAODC_22480 - Mutator family transposase, at 1,255,562 to 1,255,861
_22480
MCAODC_22485: tnp-IS3 - IS3 family transposase, at 1,255,872 to 1,256,033
tnp-IS3
MCAODC_22490: MCAODC_22490 - IS3 family transposase, at 1,256,195 to 1,257,100
_22490
Position (kb)
1255
1256 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1254.797 kb on + strand, within espK at 1254.934 kb on + strand at 1255.175 kb on + strand at 1255.185 kb on - strand at 1255.188 kb on + strand at 1255.189 kb on - strand at 1255.189 kb on - strand at 1255.189 kb on - strand at 1255.190 kb on + strand at 1255.295 kb on + strand, within MCAODC_22475 at 1255.302 kb on - strand, within MCAODC_22475 at 1255.332 kb on + strand, within MCAODC_22475 at 1255.416 kb on - strand at 1255.434 kb on - strand at 1255.497 kb on - strand at 1255.510 kb on - strand at 1255.536 kb on - strand at 1255.550 kb on - strand at 1255.825 kb on - strand, within MCAODC_22480 at 1255.831 kb on + strand, within MCAODC_22480 at 1255.831 kb on + strand, within MCAODC_22480 at 1256.009 kb on - strand, within tnp-IS3 at 1256.029 kb on - strand at 1256.031 kb on + strand at 1256.031 kb on + strand at 1256.235 kb on + strand at 1256.241 kb on + strand at 1256.241 kb on + strand at 1256.241 kb on + strand at 1256.280 kb on - strand at 1256.280 kb on - strand at 1256.341 kb on + strand, within MCAODC_22490 at 1256.341 kb on + strand, within MCAODC_22490 at 1256.361 kb on + strand, within MCAODC_22490 at 1256.362 kb on - strand, within MCAODC_22490 at 1256.390 kb on + strand, within MCAODC_22490 at 1256.390 kb on + strand, within MCAODC_22490 at 1256.391 kb on - strand, within MCAODC_22490 at 1256.443 kb on + strand, within MCAODC_22490 at 1256.520 kb on + strand, within MCAODC_22490 at 1256.542 kb on + strand, within MCAODC_22490 at 1256.542 kb on + strand, within MCAODC_22490 at 1256.543 kb on - strand, within MCAODC_22490
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,254,797 + espK MCAODC_22470 0.85 -1.2 1,254,934 + -3.7 1,255,175 + +1.0 1,255,185 - -1.3 1,255,188 + +0.8 1,255,189 - -0.3 1,255,189 - +0.7 1,255,189 - -1.2 1,255,190 + +0.8 1,255,295 + MCAODC_22475 0.46 -1.0 1,255,302 - MCAODC_22475 0.49 -0.7 1,255,332 + MCAODC_22475 0.62 -1.0 1,255,416 - -0.7 1,255,434 - +0.5 1,255,497 - -1.1 1,255,510 - -1.5 1,255,536 - -1.3 1,255,550 - +0.0 1,255,825 - MCAODC_22480 0.88 +0.3 1,255,831 + MCAODC_22480 0.90 +1.7 1,255,831 + MCAODC_22480 0.90 +0.7 1,256,009 - tnp-IS3 MCAODC_22485 0.85 -1.8 1,256,029 - -0.6 1,256,031 + +0.7 1,256,031 + -0.5 1,256,235 + -0.4 1,256,241 + +0.6 1,256,241 + -2.1 1,256,241 + -0.3 1,256,280 - +0.4 1,256,280 - +0.1 1,256,341 + MCAODC_22490 0.16 -0.2 1,256,341 + MCAODC_22490 0.16 -0.2 1,256,361 + MCAODC_22490 0.18 +1.6 1,256,362 - MCAODC_22490 0.18 -0.8 1,256,390 + MCAODC_22490 0.22 -0.4 1,256,390 + MCAODC_22490 0.22 -3.0 1,256,391 - MCAODC_22490 0.22 -0.7 1,256,443 + MCAODC_22490 0.27 -0.8 1,256,520 + MCAODC_22490 0.36 +0.7 1,256,542 + MCAODC_22490 0.38 -2.2 1,256,542 + MCAODC_22490 0.38 +0.2 1,256,543 - MCAODC_22490 0.38 +1.2
Or see this region's nucleotide sequence