Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wcaD and wcaC overlap by 26 nucleotides wcaC and wcaB overlap by 4 nucleotides wcaB and wcaA are separated by 2 nucleotides
MCAODC_20610: wcaD - putative colanic acid polymerase WcaD, at 932,926 to 934,143
wcaD
MCAODC_20615: wcaC - colanic acid biosynthesis glycosyltransferase WcaC, at 934,118 to 935,335
wcaC
MCAODC_20620: wcaB - colanic acid biosynthesis acetyltransferase WcaB, at 935,332 to 935,820
wcaB
MCAODC_20625: wcaA - colanic acid biosynthesis glycosyltransferase WcaA, at 935,823 to 936,662
wcaA
Position (kb) 934
935
936 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 933.535 kb on - strand, within wcaD at 933.671 kb on + strand, within wcaD at 933.671 kb on + strand, within wcaD at 933.799 kb on + strand, within wcaD at 933.855 kb on + strand, within wcaD at 933.873 kb on + strand, within wcaD at 933.874 kb on - strand, within wcaD at 933.874 kb on - strand, within wcaD at 933.875 kb on + strand, within wcaD at 933.888 kb on + strand, within wcaD at 933.888 kb on + strand, within wcaD at 933.888 kb on + strand, within wcaD at 933.902 kb on + strand, within wcaD at 933.902 kb on + strand, within wcaD at 933.936 kb on - strand, within wcaD at 933.940 kb on - strand, within wcaD at 934.016 kb on - strand, within wcaD at 934.094 kb on + strand at 934.095 kb on - strand at 934.115 kb on + strand at 934.115 kb on + strand at 934.143 kb on + strand at 934.144 kb on - strand at 934.144 kb on - strand at 934.144 kb on - strand at 934.144 kb on - strand at 934.501 kb on + strand, within wcaC at 934.501 kb on + strand, within wcaC at 934.501 kb on + strand, within wcaC at 934.511 kb on - strand, within wcaC at 934.565 kb on - strand, within wcaC at 934.565 kb on - strand, within wcaC at 934.596 kb on - strand, within wcaC at 934.670 kb on - strand, within wcaC at 934.675 kb on + strand, within wcaC at 934.846 kb on + strand, within wcaC at 934.846 kb on + strand, within wcaC at 934.846 kb on + strand, within wcaC at 934.847 kb on - strand, within wcaC at 934.847 kb on - strand, within wcaC at 934.847 kb on - strand, within wcaC at 935.006 kb on - strand, within wcaC at 935.069 kb on - strand, within wcaC at 935.082 kb on - strand, within wcaC at 935.161 kb on + strand, within wcaC at 935.162 kb on - strand, within wcaC at 935.182 kb on - strand, within wcaC at 935.276 kb on + strand at 935.330 kb on - strand at 935.454 kb on + strand, within wcaB at 935.588 kb on - strand, within wcaB at 935.611 kb on - strand, within wcaB at 935.678 kb on + strand, within wcaB at 935.806 kb on + strand at 935.821 kb on - strand at 935.821 kb on - strand at 935.838 kb on - strand at 935.900 kb on + strand at 935.900 kb on + strand at 935.907 kb on - strand, within wcaA at 935.907 kb on - strand, within wcaA at 935.920 kb on + strand, within wcaA at 936.119 kb on - strand, within wcaA at 936.211 kb on + strand, within wcaA at 936.229 kb on + strand, within wcaA at 936.252 kb on + strand, within wcaA at 936.271 kb on + strand, within wcaA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 933,535 - wcaD MCAODC_20610 0.50 +0.9 933,671 + wcaD MCAODC_20610 0.61 -0.3 933,671 + wcaD MCAODC_20610 0.61 +1.1 933,799 + wcaD MCAODC_20610 0.72 +0.4 933,855 + wcaD MCAODC_20610 0.76 +0.8 933,873 + wcaD MCAODC_20610 0.78 -1.4 933,874 - wcaD MCAODC_20610 0.78 -0.3 933,874 - wcaD MCAODC_20610 0.78 -0.2 933,875 + wcaD MCAODC_20610 0.78 -0.9 933,888 + wcaD MCAODC_20610 0.79 -0.9 933,888 + wcaD MCAODC_20610 0.79 +0.9 933,888 + wcaD MCAODC_20610 0.79 -0.8 933,902 + wcaD MCAODC_20610 0.80 -1.9 933,902 + wcaD MCAODC_20610 0.80 -0.3 933,936 - wcaD MCAODC_20610 0.83 -0.9 933,940 - wcaD MCAODC_20610 0.83 +0.4 934,016 - wcaD MCAODC_20610 0.89 +1.5 934,094 + +1.0 934,095 - +0.5 934,115 + +0.6 934,115 + -0.9 934,143 + +1.5 934,144 - +0.7 934,144 - -0.3 934,144 - -1.1 934,144 - -0.0 934,501 + wcaC MCAODC_20615 0.31 -0.6 934,501 + wcaC MCAODC_20615 0.31 -0.3 934,501 + wcaC MCAODC_20615 0.31 -0.8 934,511 - wcaC MCAODC_20615 0.32 +0.0 934,565 - wcaC MCAODC_20615 0.37 +1.0 934,565 - wcaC MCAODC_20615 0.37 -0.2 934,596 - wcaC MCAODC_20615 0.39 +2.0 934,670 - wcaC MCAODC_20615 0.45 -0.2 934,675 + wcaC MCAODC_20615 0.46 +0.1 934,846 + wcaC MCAODC_20615 0.60 -0.6 934,846 + wcaC MCAODC_20615 0.60 +0.2 934,846 + wcaC MCAODC_20615 0.60 +0.2 934,847 - wcaC MCAODC_20615 0.60 -0.9 934,847 - wcaC MCAODC_20615 0.60 -1.3 934,847 - wcaC MCAODC_20615 0.60 +0.7 935,006 - wcaC MCAODC_20615 0.73 +0.9 935,069 - wcaC MCAODC_20615 0.78 -0.1 935,082 - wcaC MCAODC_20615 0.79 +0.8 935,161 + wcaC MCAODC_20615 0.86 -1.1 935,162 - wcaC MCAODC_20615 0.86 -0.2 935,182 - wcaC MCAODC_20615 0.87 -0.4 935,276 + +0.9 935,330 - +1.0 935,454 + wcaB MCAODC_20620 0.25 +1.2 935,588 - wcaB MCAODC_20620 0.52 -1.6 935,611 - wcaB MCAODC_20620 0.57 +1.0 935,678 + wcaB MCAODC_20620 0.71 +1.3 935,806 + -2.7 935,821 - -1.0 935,821 - +0.1 935,838 - -0.9 935,900 + +0.9 935,900 + -0.1 935,907 - wcaA MCAODC_20625 0.10 +1.0 935,907 - wcaA MCAODC_20625 0.10 -0.0 935,920 + wcaA MCAODC_20625 0.12 -0.9 936,119 - wcaA MCAODC_20625 0.35 -2.2 936,211 + wcaA MCAODC_20625 0.46 -0.3 936,229 + wcaA MCAODC_20625 0.48 -0.1 936,252 + wcaA MCAODC_20625 0.51 -0.6 936,271 + wcaA MCAODC_20625 0.53 -3.2
Or see this region's nucleotide sequence