Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MCAODC_20540 and wcaM are separated by 157 nucleotides wcaM and wcaL are separated by 10 nucleotides
MCAODC_20540: MCAODC_20540 - N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,o ctacis-undecaprenol 4-epimerase, at 916,619 to 917,614
_20540
MCAODC_20545: wcaM - colanic acid biosynthesis protein WcaM, at 917,772 to 919,166
wcaM
MCAODC_20550: wcaL - colanic acid biosynthesis glycosyltransferase WcaL, at 919,177 to 920,397
wcaL
Position (kb) 917
918
919
920 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 916.871 kb on - strand, within MCAODC_20540 at 916.871 kb on - strand, within MCAODC_20540 at 916.871 kb on - strand, within MCAODC_20540 at 916.940 kb on - strand, within MCAODC_20540 at 916.948 kb on - strand, within MCAODC_20540 at 916.952 kb on - strand, within MCAODC_20540 at 916.952 kb on - strand, within MCAODC_20540 at 916.952 kb on - strand, within MCAODC_20540 at 916.952 kb on - strand, within MCAODC_20540 at 916.954 kb on - strand, within MCAODC_20540 at 917.020 kb on - strand, within MCAODC_20540 at 917.020 kb on - strand, within MCAODC_20540 at 917.020 kb on - strand, within MCAODC_20540 at 917.020 kb on - strand, within MCAODC_20540 at 917.020 kb on - strand, within MCAODC_20540 at 917.032 kb on + strand, within MCAODC_20540 at 917.033 kb on - strand, within MCAODC_20540 at 917.033 kb on - strand, within MCAODC_20540 at 917.033 kb on - strand, within MCAODC_20540 at 917.033 kb on - strand, within MCAODC_20540 at 917.034 kb on + strand, within MCAODC_20540 at 917.035 kb on - strand, within MCAODC_20540 at 917.038 kb on + strand, within MCAODC_20540 at 917.039 kb on - strand, within MCAODC_20540 at 917.059 kb on + strand, within MCAODC_20540 at 917.060 kb on - strand, within MCAODC_20540 at 917.060 kb on - strand, within MCAODC_20540 at 917.060 kb on - strand, within MCAODC_20540 at 917.092 kb on + strand, within MCAODC_20540 at 917.092 kb on + strand, within MCAODC_20540 at 917.093 kb on - strand, within MCAODC_20540 at 917.093 kb on - strand, within MCAODC_20540 at 917.093 kb on - strand, within MCAODC_20540 at 917.093 kb on - strand, within MCAODC_20540 at 917.125 kb on + strand, within MCAODC_20540 at 917.143 kb on + strand, within MCAODC_20540 at 917.144 kb on - strand, within MCAODC_20540 at 917.147 kb on + strand, within MCAODC_20540 at 917.148 kb on - strand, within MCAODC_20540 at 917.148 kb on - strand, within MCAODC_20540 at 917.148 kb on - strand, within MCAODC_20540 at 917.174 kb on - strand, within MCAODC_20540 at 917.174 kb on - strand, within MCAODC_20540 at 917.174 kb on - strand, within MCAODC_20540 at 917.174 kb on - strand, within MCAODC_20540 at 917.174 kb on - strand, within MCAODC_20540 at 917.174 kb on - strand, within MCAODC_20540 at 917.278 kb on - strand, within MCAODC_20540 at 917.371 kb on - strand, within MCAODC_20540 at 917.371 kb on - strand, within MCAODC_20540 at 917.371 kb on - strand, within MCAODC_20540 at 917.373 kb on + strand, within MCAODC_20540 at 917.373 kb on + strand, within MCAODC_20540 at 917.384 kb on - strand, within MCAODC_20540 at 917.421 kb on - strand, within MCAODC_20540 at 917.592 kb on - strand at 917.625 kb on - strand at 917.625 kb on - strand at 917.636 kb on + strand at 917.636 kb on + strand at 917.636 kb on + strand at 917.636 kb on + strand at 917.637 kb on - strand at 917.641 kb on - strand at 917.654 kb on + strand at 917.654 kb on + strand at 917.664 kb on + strand at 917.664 kb on + strand at 917.665 kb on - strand at 917.710 kb on + strand at 917.711 kb on - strand at 917.725 kb on - strand at 917.725 kb on - strand at 917.741 kb on - strand at 917.741 kb on - strand at 917.773 kb on + strand at 917.774 kb on - strand at 918.065 kb on + strand, within wcaM at 918.065 kb on + strand, within wcaM at 918.066 kb on - strand, within wcaM at 918.124 kb on + strand, within wcaM at 918.156 kb on - strand, within wcaM at 918.208 kb on + strand, within wcaM at 918.216 kb on + strand, within wcaM at 918.275 kb on - strand, within wcaM at 918.348 kb on - strand, within wcaM at 918.382 kb on - strand, within wcaM at 918.382 kb on - strand, within wcaM at 918.382 kb on - strand, within wcaM at 918.456 kb on - strand, within wcaM at 918.456 kb on - strand, within wcaM at 918.456 kb on - strand, within wcaM at 918.459 kb on - strand, within wcaM at 918.463 kb on + strand, within wcaM at 918.463 kb on + strand, within wcaM at 918.464 kb on - strand, within wcaM at 918.464 kb on - strand, within wcaM at 918.483 kb on + strand, within wcaM at 918.484 kb on - strand, within wcaM at 918.555 kb on - strand, within wcaM at 918.673 kb on + strand, within wcaM at 918.674 kb on - strand, within wcaM at 918.745 kb on + strand, within wcaM at 918.827 kb on + strand, within wcaM at 918.827 kb on + strand, within wcaM at 918.827 kb on + strand, within wcaM at 918.828 kb on - strand, within wcaM at 918.834 kb on - strand, within wcaM at 918.834 kb on - strand, within wcaM at 918.921 kb on + strand, within wcaM at 918.960 kb on - strand, within wcaM at 919.019 kb on + strand, within wcaM at 919.019 kb on + strand, within wcaM at 919.048 kb on + strand at 919.049 kb on - strand at 919.158 kb on - strand at 919.166 kb on + strand at 919.167 kb on - strand at 919.167 kb on - strand at 919.178 kb on + strand at 919.179 kb on - strand at 919.398 kb on + strand, within wcaL at 919.459 kb on - strand, within wcaL at 919.459 kb on - strand, within wcaL at 919.574 kb on + strand, within wcaL at 919.777 kb on - strand, within wcaL at 919.777 kb on - strand, within wcaL at 919.777 kb on - strand, within wcaL at 919.781 kb on - strand, within wcaL at 919.781 kb on - strand, within wcaL at 919.781 kb on - strand, within wcaL at 919.907 kb on + strand, within wcaL at 919.907 kb on + strand, within wcaL at 919.908 kb on - strand, within wcaL at 919.935 kb on + strand, within wcaL at 919.936 kb on - strand, within wcaL at 919.936 kb on - strand, within wcaL at 920.136 kb on + strand, within wcaL
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 916,871 - MCAODC_20540 0.25 +0.6 916,871 - MCAODC_20540 0.25 -0.4 916,871 - MCAODC_20540 0.25 +0.5 916,940 - MCAODC_20540 0.32 +0.4 916,948 - MCAODC_20540 0.33 +1.1 916,952 - MCAODC_20540 0.33 +0.7 916,952 - MCAODC_20540 0.33 +2.2 916,952 - MCAODC_20540 0.33 +0.9 916,952 - MCAODC_20540 0.33 +0.7 916,954 - MCAODC_20540 0.34 +1.7 917,020 - MCAODC_20540 0.40 +1.0 917,020 - MCAODC_20540 0.40 +1.2 917,020 - MCAODC_20540 0.40 +0.5 917,020 - MCAODC_20540 0.40 +0.7 917,020 - MCAODC_20540 0.40 +0.6 917,032 + MCAODC_20540 0.41 +0.6 917,033 - MCAODC_20540 0.42 +0.8 917,033 - MCAODC_20540 0.42 +0.8 917,033 - MCAODC_20540 0.42 +1.0 917,033 - MCAODC_20540 0.42 +1.1 917,034 + MCAODC_20540 0.42 +1.7 917,035 - MCAODC_20540 0.42 +0.9 917,038 + MCAODC_20540 0.42 +1.6 917,039 - MCAODC_20540 0.42 +0.4 917,059 + MCAODC_20540 0.44 +1.8 917,060 - MCAODC_20540 0.44 +0.8 917,060 - MCAODC_20540 0.44 +0.2 917,060 - MCAODC_20540 0.44 +0.8 917,092 + MCAODC_20540 0.47 -2.8 917,092 + MCAODC_20540 0.47 +0.1 917,093 - MCAODC_20540 0.48 +0.6 917,093 - MCAODC_20540 0.48 +0.7 917,093 - MCAODC_20540 0.48 -1.1 917,093 - MCAODC_20540 0.48 +0.2 917,125 + MCAODC_20540 0.51 +1.6 917,143 + MCAODC_20540 0.53 -1.1 917,144 - MCAODC_20540 0.53 +0.6 917,147 + MCAODC_20540 0.53 +0.4 917,148 - MCAODC_20540 0.53 +0.4 917,148 - MCAODC_20540 0.53 -0.3 917,148 - MCAODC_20540 0.53 +0.2 917,174 - MCAODC_20540 0.56 +1.0 917,174 - MCAODC_20540 0.56 +0.0 917,174 - MCAODC_20540 0.56 +0.9 917,174 - MCAODC_20540 0.56 +1.2 917,174 - MCAODC_20540 0.56 +0.4 917,174 - MCAODC_20540 0.56 -0.7 917,278 - MCAODC_20540 0.66 -1.2 917,371 - MCAODC_20540 0.76 -1.0 917,371 - MCAODC_20540 0.76 -0.7 917,371 - MCAODC_20540 0.76 +0.6 917,373 + MCAODC_20540 0.76 +0.6 917,373 + MCAODC_20540 0.76 +0.2 917,384 - MCAODC_20540 0.77 +1.4 917,421 - MCAODC_20540 0.81 -0.9 917,592 - +1.4 917,625 - +0.4 917,625 - -0.1 917,636 + -0.2 917,636 + +1.0 917,636 + -0.3 917,636 + +0.5 917,637 - -1.0 917,641 - -0.8 917,654 + -0.1 917,654 + -1.1 917,664 + +1.8 917,664 + -0.9 917,665 - +0.3 917,710 + +0.7 917,711 - +0.5 917,725 - -0.3 917,725 - +0.0 917,741 - +0.5 917,741 - -0.5 917,773 + +1.6 917,774 - +1.0 918,065 + wcaM MCAODC_20545 0.21 -3.4 918,065 + wcaM MCAODC_20545 0.21 +1.5 918,066 - wcaM MCAODC_20545 0.21 -0.8 918,124 + wcaM MCAODC_20545 0.25 +0.9 918,156 - wcaM MCAODC_20545 0.28 -1.3 918,208 + wcaM MCAODC_20545 0.31 -2.4 918,216 + wcaM MCAODC_20545 0.32 +0.8 918,275 - wcaM MCAODC_20545 0.36 -2.5 918,348 - wcaM MCAODC_20545 0.41 +0.6 918,382 - wcaM MCAODC_20545 0.44 +0.1 918,382 - wcaM MCAODC_20545 0.44 -1.0 918,382 - wcaM MCAODC_20545 0.44 +0.1 918,456 - wcaM MCAODC_20545 0.49 -0.0 918,456 - wcaM MCAODC_20545 0.49 +0.1 918,456 - wcaM MCAODC_20545 0.49 +0.4 918,459 - wcaM MCAODC_20545 0.49 +1.2 918,463 + wcaM MCAODC_20545 0.50 +0.3 918,463 + wcaM MCAODC_20545 0.50 +0.2 918,464 - wcaM MCAODC_20545 0.50 +0.6 918,464 - wcaM MCAODC_20545 0.50 -0.1 918,483 + wcaM MCAODC_20545 0.51 -0.2 918,484 - wcaM MCAODC_20545 0.51 +0.3 918,555 - wcaM MCAODC_20545 0.56 -2.0 918,673 + wcaM MCAODC_20545 0.65 +2.8 918,674 - wcaM MCAODC_20545 0.65 -1.5 918,745 + wcaM MCAODC_20545 0.70 +0.4 918,827 + wcaM MCAODC_20545 0.76 +0.6 918,827 + wcaM MCAODC_20545 0.76 +0.8 918,827 + wcaM MCAODC_20545 0.76 +0.5 918,828 - wcaM MCAODC_20545 0.76 -0.5 918,834 - wcaM MCAODC_20545 0.76 +0.5 918,834 - wcaM MCAODC_20545 0.76 +1.3 918,921 + wcaM MCAODC_20545 0.82 -0.7 918,960 - wcaM MCAODC_20545 0.85 -0.2 919,019 + wcaM MCAODC_20545 0.89 +0.3 919,019 + wcaM MCAODC_20545 0.89 -0.6 919,048 + +0.1 919,049 - +0.4 919,158 - -0.2 919,166 + -0.4 919,167 - +0.4 919,167 - -0.7 919,178 + +0.2 919,179 - -0.2 919,398 + wcaL MCAODC_20550 0.18 +2.2 919,459 - wcaL MCAODC_20550 0.23 +1.2 919,459 - wcaL MCAODC_20550 0.23 +0.2 919,574 + wcaL MCAODC_20550 0.33 -0.3 919,777 - wcaL MCAODC_20550 0.49 -1.5 919,777 - wcaL MCAODC_20550 0.49 -0.5 919,777 - wcaL MCAODC_20550 0.49 -0.6 919,781 - wcaL MCAODC_20550 0.49 -1.4 919,781 - wcaL MCAODC_20550 0.49 +0.4 919,781 - wcaL MCAODC_20550 0.49 -0.5 919,907 + wcaL MCAODC_20550 0.60 -2.0 919,907 + wcaL MCAODC_20550 0.60 +1.1 919,908 - wcaL MCAODC_20550 0.60 -0.2 919,935 + wcaL MCAODC_20550 0.62 +0.9 919,936 - wcaL MCAODC_20550 0.62 +0.8 919,936 - wcaL MCAODC_20550 0.62 -0.6 920,136 + wcaL MCAODC_20550 0.79 -3.2
Or see this region's nucleotide sequence