Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cfa and ribE are separated by 39 nucleotides ribE and mdtK are separated by 214 nucleotides mdtK and ydhQ are separated by 40 nucleotides
MCAODC_18040: cfa - cyclopropane fatty acyl phospholipid synthase, at 470,393 to 471,541
cfa
MCAODC_18045: ribE - riboflavin synthase, at 471,581 to 472,222
ribE
MCAODC_18050: mdtK - multidrug efflux MATE transporter MdtK, at 472,437 to 473,810
mdtK
MCAODC_18055: ydhQ - Uncharacterized protein YdhQ, at 473,851 to 475,107
ydhQ
Position (kb) 472
473
474 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 471.437 kb on + strand at 471.438 kb on - strand at 471.552 kb on - strand at 471.639 kb on - strand at 472.246 kb on - strand at 472.246 kb on - strand at 472.287 kb on + strand at 472.345 kb on - strand at 472.349 kb on + strand at 472.349 kb on + strand at 472.411 kb on + strand at 472.414 kb on - strand at 472.414 kb on - strand at 472.417 kb on + strand at 472.424 kb on - strand at 472.447 kb on - strand at 472.448 kb on + strand at 472.471 kb on + strand at 472.593 kb on - strand, within mdtK at 472.660 kb on + strand, within mdtK at 472.962 kb on + strand, within mdtK at 472.962 kb on + strand, within mdtK at 472.962 kb on + strand, within mdtK at 472.962 kb on + strand, within mdtK at 472.963 kb on - strand, within mdtK at 472.963 kb on - strand, within mdtK at 472.963 kb on - strand, within mdtK at 473.037 kb on + strand, within mdtK at 473.037 kb on + strand, within mdtK at 473.067 kb on + strand, within mdtK at 473.067 kb on + strand, within mdtK at 473.221 kb on + strand, within mdtK at 473.394 kb on - strand, within mdtK at 473.466 kb on + strand, within mdtK at 473.578 kb on - strand, within mdtK at 473.601 kb on + strand, within mdtK at 473.647 kb on - strand, within mdtK at 473.682 kb on - strand at 473.711 kb on + strand at 473.738 kb on + strand at 474.250 kb on + strand, within ydhQ at 474.353 kb on - strand, within ydhQ at 474.353 kb on - strand, within ydhQ at 474.353 kb on - strand, within ydhQ at 474.406 kb on - strand, within ydhQ at 474.438 kb on - strand, within ydhQ at 474.468 kb on - strand, within ydhQ at 474.489 kb on + strand, within ydhQ at 474.490 kb on - strand, within ydhQ at 474.543 kb on + strand, within ydhQ at 474.544 kb on - strand, within ydhQ at 474.544 kb on - strand, within ydhQ at 474.544 kb on - strand, within ydhQ at 474.644 kb on - strand, within ydhQ at 474.644 kb on - strand, within ydhQ at 474.770 kb on + strand, within ydhQ at 474.770 kb on + strand, within ydhQ at 474.771 kb on - strand, within ydhQ at 474.771 kb on - strand, within ydhQ
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 471,437 + -2.2 471,438 - +0.7 471,552 - +0.3 471,639 - +0.5 472,246 - -0.1 472,246 - +0.7 472,287 + -1.1 472,345 - -0.9 472,349 + -0.2 472,349 + +1.9 472,411 + +0.5 472,414 - +1.7 472,414 - -0.8 472,417 + +0.8 472,424 - -2.4 472,447 - +0.4 472,448 + +0.4 472,471 + -0.1 472,593 - mdtK MCAODC_18050 0.11 -2.0 472,660 + mdtK MCAODC_18050 0.16 -0.8 472,962 + mdtK MCAODC_18050 0.38 -0.4 472,962 + mdtK MCAODC_18050 0.38 +0.3 472,962 + mdtK MCAODC_18050 0.38 -1.6 472,962 + mdtK MCAODC_18050 0.38 +1.4 472,963 - mdtK MCAODC_18050 0.38 -0.6 472,963 - mdtK MCAODC_18050 0.38 +0.7 472,963 - mdtK MCAODC_18050 0.38 -0.3 473,037 + mdtK MCAODC_18050 0.44 +0.7 473,037 + mdtK MCAODC_18050 0.44 +0.3 473,067 + mdtK MCAODC_18050 0.46 +0.9 473,067 + mdtK MCAODC_18050 0.46 +0.3 473,221 + mdtK MCAODC_18050 0.57 -1.4 473,394 - mdtK MCAODC_18050 0.70 -2.3 473,466 + mdtK MCAODC_18050 0.75 -1.5 473,578 - mdtK MCAODC_18050 0.83 +0.5 473,601 + mdtK MCAODC_18050 0.85 -0.8 473,647 - mdtK MCAODC_18050 0.88 +0.1 473,682 - -0.2 473,711 + +0.3 473,738 + +0.9 474,250 + ydhQ MCAODC_18055 0.32 -0.2 474,353 - ydhQ MCAODC_18055 0.40 +0.6 474,353 - ydhQ MCAODC_18055 0.40 -2.4 474,353 - ydhQ MCAODC_18055 0.40 -3.2 474,406 - ydhQ MCAODC_18055 0.44 -0.5 474,438 - ydhQ MCAODC_18055 0.47 +1.0 474,468 - ydhQ MCAODC_18055 0.49 -2.2 474,489 + ydhQ MCAODC_18055 0.51 -0.6 474,490 - ydhQ MCAODC_18055 0.51 +0.6 474,543 + ydhQ MCAODC_18055 0.55 -1.6 474,544 - ydhQ MCAODC_18055 0.55 -2.6 474,544 - ydhQ MCAODC_18055 0.55 +0.6 474,544 - ydhQ MCAODC_18055 0.55 +0.5 474,644 - ydhQ MCAODC_18055 0.63 -1.1 474,644 - ydhQ MCAODC_18055 0.63 -1.7 474,770 + ydhQ MCAODC_18055 0.73 +0.4 474,770 + ydhQ MCAODC_18055 0.73 -0.4 474,771 - ydhQ MCAODC_18055 0.73 +0.6 474,771 - ydhQ MCAODC_18055 0.73 -1.3
Or see this region's nucleotide sequence