Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nth and MCAODC_17890 are separated by 127 nucleotides MCAODC_17890 and dtpA are separated by 282 nucleotides dtpA and gstA are separated by 105 nucleotides gstA and pdxY are separated by 43 nucleotides
MCAODC_17885: nth - endonuclease III, at 440,519 to 441,154
nth
MCAODC_17890: MCAODC_17890 - hypothetical protein, at 441,282 to 441,482
_17890
MCAODC_17895: dtpA - dipeptide/tripeptide permease DtpA, at 441,765 to 443,267
dtpA
MCAODC_17900: gstA - glutathione transferase GstA, at 443,373 to 443,978
gstA
MCAODC_17905: pdxY - pyridoxal kinase PdxY, at 444,022 to 444,882
pdxY
Position (kb) 441
442
443
444 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 440.765 kb on - strand, within nth at 440.782 kb on + strand, within nth at 440.895 kb on + strand, within nth at 440.953 kb on + strand, within nth at 441.073 kb on + strand, within nth at 441.110 kb on - strand at 441.131 kb on + strand at 441.198 kb on - strand at 441.259 kb on - strand at 441.663 kb on - strand at 441.684 kb on + strand at 441.684 kb on + strand at 441.714 kb on + strand at 441.834 kb on + strand at 441.835 kb on - strand at 441.893 kb on + strand at 442.003 kb on + strand, within dtpA at 442.025 kb on + strand, within dtpA at 442.037 kb on + strand, within dtpA at 442.069 kb on - strand, within dtpA at 442.110 kb on + strand, within dtpA at 442.185 kb on + strand, within dtpA at 442.227 kb on + strand, within dtpA at 442.227 kb on + strand, within dtpA at 442.227 kb on + strand, within dtpA at 442.228 kb on - strand, within dtpA at 442.231 kb on - strand, within dtpA at 442.349 kb on + strand, within dtpA at 442.417 kb on - strand, within dtpA at 442.439 kb on + strand, within dtpA at 442.502 kb on + strand, within dtpA at 442.622 kb on + strand, within dtpA at 442.622 kb on + strand, within dtpA at 442.638 kb on + strand, within dtpA at 442.691 kb on + strand, within dtpA at 442.725 kb on + strand, within dtpA at 442.782 kb on + strand, within dtpA at 442.783 kb on - strand, within dtpA at 442.784 kb on + strand, within dtpA at 443.134 kb on - strand at 443.348 kb on - strand at 443.418 kb on - strand at 443.572 kb on + strand, within gstA at 443.671 kb on - strand, within gstA at 443.691 kb on - strand, within gstA at 443.733 kb on + strand, within gstA at 443.775 kb on + strand, within gstA at 443.841 kb on + strand, within gstA at 443.842 kb on - strand, within gstA at 443.855 kb on - strand, within gstA at 443.915 kb on + strand, within gstA at 443.924 kb on + strand at 443.924 kb on + strand at 443.979 kb on + strand at 444.063 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 440,765 - nth MCAODC_17885 0.39 +0.1 440,782 + nth MCAODC_17885 0.41 +0.8 440,895 + nth MCAODC_17885 0.59 -0.0 440,953 + nth MCAODC_17885 0.68 -2.4 441,073 + nth MCAODC_17885 0.87 -1.0 441,110 - -0.2 441,131 + +2.0 441,198 - +0.3 441,259 - -1.6 441,663 - +0.8 441,684 + +0.2 441,684 + -0.5 441,714 + +1.3 441,834 + -1.5 441,835 - +1.1 441,893 + +0.4 442,003 + dtpA MCAODC_17895 0.16 +1.5 442,025 + dtpA MCAODC_17895 0.17 +0.3 442,037 + dtpA MCAODC_17895 0.18 -1.0 442,069 - dtpA MCAODC_17895 0.20 -0.1 442,110 + dtpA MCAODC_17895 0.23 -1.6 442,185 + dtpA MCAODC_17895 0.28 -0.9 442,227 + dtpA MCAODC_17895 0.31 +1.3 442,227 + dtpA MCAODC_17895 0.31 -2.1 442,227 + dtpA MCAODC_17895 0.31 +1.9 442,228 - dtpA MCAODC_17895 0.31 +0.6 442,231 - dtpA MCAODC_17895 0.31 +1.4 442,349 + dtpA MCAODC_17895 0.39 -0.6 442,417 - dtpA MCAODC_17895 0.43 +0.2 442,439 + dtpA MCAODC_17895 0.45 +1.7 442,502 + dtpA MCAODC_17895 0.49 -3.0 442,622 + dtpA MCAODC_17895 0.57 -1.1 442,622 + dtpA MCAODC_17895 0.57 -2.1 442,638 + dtpA MCAODC_17895 0.58 +1.9 442,691 + dtpA MCAODC_17895 0.62 -1.3 442,725 + dtpA MCAODC_17895 0.64 +0.8 442,782 + dtpA MCAODC_17895 0.68 -0.2 442,783 - dtpA MCAODC_17895 0.68 -0.9 442,784 + dtpA MCAODC_17895 0.68 +0.5 443,134 - +0.3 443,348 - -0.2 443,418 - -0.3 443,572 + gstA MCAODC_17900 0.33 +0.1 443,671 - gstA MCAODC_17900 0.49 -0.9 443,691 - gstA MCAODC_17900 0.52 -0.9 443,733 + gstA MCAODC_17900 0.59 -0.1 443,775 + gstA MCAODC_17900 0.66 +0.5 443,841 + gstA MCAODC_17900 0.77 -0.1 443,842 - gstA MCAODC_17900 0.77 -1.2 443,855 - gstA MCAODC_17900 0.80 +0.4 443,915 + gstA MCAODC_17900 0.89 +1.3 443,924 + -2.7 443,924 + +1.0 443,979 + -0.3 444,063 + -4.8
Or see this region's nucleotide sequence