Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MCAODC_16400 and mddA overlap by 4 nucleotides mddA and curA are separated by 180 nucleotides curA and mcbR are separated by 197 nucleotides mcbR and yncD are separated by 35 nucleotides
MCAODC_16400: MCAODC_16400 - Putative inner membrane exporter, YdcZ, at 171,209 to 171,658
_16400
MCAODC_16405: mddA - L-methionine sulfoximine/L-methionine sulfone acetyltransferase, at 171,655 to 172,173
mddA
MCAODC_16410: curA - NADPH-dependent curcumin/dihydrocurcumin reductase, at 172,354 to 173,391
curA
MCAODC_16415: mcbR - colanic acid/biofilm transcriptional regulator McbR, at 173,589 to 174,254
mcbR
MCAODC_16420: yncD - putative TonB-dependent receptor YncD, at 174,290 to 176,392
yncD
Position (kb) 172
173
174 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 171.361 kb on + strand, within MCAODC_16400 at 171.362 kb on - strand, within MCAODC_16400 at 171.435 kb on - strand, within MCAODC_16400 at 171.437 kb on - strand, within MCAODC_16400 at 171.437 kb on - strand, within MCAODC_16400 at 171.437 kb on - strand, within MCAODC_16400 at 171.485 kb on - strand, within MCAODC_16400 at 171.703 kb on - strand at 172.009 kb on + strand, within mddA at 172.031 kb on - strand, within mddA at 172.102 kb on - strand, within mddA at 172.103 kb on + strand, within mddA at 172.103 kb on + strand, within mddA at 172.192 kb on - strand at 172.192 kb on - strand at 172.192 kb on - strand at 172.212 kb on + strand at 172.212 kb on + strand at 172.318 kb on - strand at 172.479 kb on - strand, within curA at 172.510 kb on + strand, within curA at 172.510 kb on + strand, within curA at 172.511 kb on - strand, within curA at 172.512 kb on + strand, within curA at 172.512 kb on + strand, within curA at 172.512 kb on + strand, within curA at 172.524 kb on + strand, within curA at 172.524 kb on + strand, within curA at 172.524 kb on + strand, within curA at 172.543 kb on + strand, within curA at 172.544 kb on - strand, within curA at 172.673 kb on + strand, within curA at 172.767 kb on + strand, within curA at 172.767 kb on + strand, within curA at 172.767 kb on + strand, within curA at 172.775 kb on + strand, within curA at 172.775 kb on + strand, within curA at 172.775 kb on + strand, within curA at 172.794 kb on + strand, within curA at 172.818 kb on - strand, within curA at 173.013 kb on + strand, within curA at 173.017 kb on + strand, within curA at 173.038 kb on - strand, within curA at 173.070 kb on + strand, within curA at 173.110 kb on + strand, within curA at 173.111 kb on - strand, within curA at 173.113 kb on - strand, within curA at 173.126 kb on + strand, within curA at 173.147 kb on + strand, within curA at 173.163 kb on + strand, within curA at 173.163 kb on + strand, within curA at 173.306 kb on + strand at 173.460 kb on - strand at 173.521 kb on + strand at 173.660 kb on + strand, within mcbR at 173.719 kb on + strand, within mcbR at 173.729 kb on + strand, within mcbR at 173.752 kb on + strand, within mcbR at 173.870 kb on - strand, within mcbR at 174.244 kb on - strand at 174.351 kb on + strand at 174.355 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 171,361 + MCAODC_16400 0.34 -1.9 171,362 - MCAODC_16400 0.34 -2.8 171,435 - MCAODC_16400 0.50 -0.5 171,437 - MCAODC_16400 0.51 +2.8 171,437 - MCAODC_16400 0.51 +0.4 171,437 - MCAODC_16400 0.51 -0.6 171,485 - MCAODC_16400 0.61 +1.0 171,703 - +1.0 172,009 + mddA MCAODC_16405 0.68 -0.3 172,031 - mddA MCAODC_16405 0.72 +0.9 172,102 - mddA MCAODC_16405 0.86 +0.8 172,103 + mddA MCAODC_16405 0.86 +0.1 172,103 + mddA MCAODC_16405 0.86 -1.7 172,192 - -2.2 172,192 - +0.8 172,192 - +0.4 172,212 + -0.7 172,212 + -0.1 172,318 - +1.1 172,479 - curA MCAODC_16410 0.12 -2.1 172,510 + curA MCAODC_16410 0.15 +0.2 172,510 + curA MCAODC_16410 0.15 +1.0 172,511 - curA MCAODC_16410 0.15 -0.2 172,512 + curA MCAODC_16410 0.15 -2.8 172,512 + curA MCAODC_16410 0.15 +0.4 172,512 + curA MCAODC_16410 0.15 -0.8 172,524 + curA MCAODC_16410 0.16 +1.1 172,524 + curA MCAODC_16410 0.16 +1.6 172,524 + curA MCAODC_16410 0.16 -0.9 172,543 + curA MCAODC_16410 0.18 +0.5 172,544 - curA MCAODC_16410 0.18 -0.3 172,673 + curA MCAODC_16410 0.31 -0.4 172,767 + curA MCAODC_16410 0.40 -0.4 172,767 + curA MCAODC_16410 0.40 -0.4 172,767 + curA MCAODC_16410 0.40 -1.5 172,775 + curA MCAODC_16410 0.41 -0.9 172,775 + curA MCAODC_16410 0.41 +1.0 172,775 + curA MCAODC_16410 0.41 -0.3 172,794 + curA MCAODC_16410 0.42 +0.2 172,818 - curA MCAODC_16410 0.45 -0.2 173,013 + curA MCAODC_16410 0.63 +0.3 173,017 + curA MCAODC_16410 0.64 -0.7 173,038 - curA MCAODC_16410 0.66 -0.3 173,070 + curA MCAODC_16410 0.69 -0.8 173,110 + curA MCAODC_16410 0.73 +0.4 173,111 - curA MCAODC_16410 0.73 +0.1 173,113 - curA MCAODC_16410 0.73 -1.4 173,126 + curA MCAODC_16410 0.74 -1.8 173,147 + curA MCAODC_16410 0.76 +2.2 173,163 + curA MCAODC_16410 0.78 +0.3 173,163 + curA MCAODC_16410 0.78 +0.4 173,306 + -1.1 173,460 - +0.9 173,521 + +0.3 173,660 + mcbR MCAODC_16415 0.11 -3.1 173,719 + mcbR MCAODC_16415 0.20 -1.0 173,729 + mcbR MCAODC_16415 0.21 -0.7 173,752 + mcbR MCAODC_16415 0.24 +1.6 173,870 - mcbR MCAODC_16415 0.42 +1.4 174,244 - -0.8 174,351 + -0.2 174,355 - -0.4
Or see this region's nucleotide sequence