Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt patD and ortT are separated by 386 nucleotides ortT and ydcY are separated by 85 nucleotides ydcY and MCAODC_16400 are separated by 0 nucleotides MCAODC_16400 and mddA overlap by 4 nucleotides mddA and curA are separated by 180 nucleotides
MCAODC_16385: patD - aminobutyraldehyde dehydrogenase, at 168,905 to 170,329
patD
MCAODC_16390: ortT - orphan toxin OrtT, at 170,716 to 170,889
ortT
MCAODC_16395: ydcY - Uncharacterized protein YdcY, at 170,975 to 171,208
ydcY
MCAODC_16400: MCAODC_16400 - Putative inner membrane exporter, YdcZ, at 171,209 to 171,658
_16400
MCAODC_16405: mddA - L-methionine sulfoximine/L-methionine sulfone acetyltransferase, at 171,655 to 172,173
mddA
MCAODC_16410: curA - NADPH-dependent curcumin/dihydrocurcumin reductase, at 172,354 to 173,391
curA
Position (kb) 171
172 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 170.220 kb on + strand at 170.270 kb on - strand at 170.328 kb on - strand at 170.349 kb on - strand at 170.456 kb on - strand at 170.456 kb on - strand at 170.456 kb on - strand at 170.456 kb on - strand at 170.456 kb on - strand at 170.490 kb on - strand at 170.495 kb on + strand at 170.496 kb on - strand at 170.498 kb on + strand at 170.498 kb on + strand at 170.499 kb on - strand at 170.499 kb on - strand at 170.548 kb on - strand at 170.554 kb on - strand at 170.554 kb on - strand at 170.556 kb on - strand at 170.556 kb on - strand at 170.556 kb on - strand at 170.559 kb on - strand at 170.583 kb on + strand at 170.927 kb on + strand at 171.051 kb on + strand, within ydcY at 171.098 kb on + strand, within ydcY at 171.151 kb on + strand, within ydcY at 171.151 kb on + strand, within ydcY at 171.151 kb on + strand, within ydcY at 171.151 kb on + strand, within ydcY at 171.152 kb on - strand, within ydcY at 171.361 kb on + strand, within MCAODC_16400 at 171.362 kb on - strand, within MCAODC_16400 at 171.435 kb on - strand, within MCAODC_16400 at 171.437 kb on - strand, within MCAODC_16400 at 171.437 kb on - strand, within MCAODC_16400 at 171.437 kb on - strand, within MCAODC_16400 at 171.485 kb on - strand, within MCAODC_16400 at 171.703 kb on - strand at 172.009 kb on + strand, within mddA at 172.031 kb on - strand, within mddA at 172.102 kb on - strand, within mddA at 172.103 kb on + strand, within mddA at 172.103 kb on + strand, within mddA at 172.192 kb on - strand at 172.192 kb on - strand at 172.192 kb on - strand at 172.212 kb on + strand at 172.212 kb on + strand at 172.318 kb on - strand at 172.479 kb on - strand, within curA at 172.510 kb on + strand, within curA at 172.510 kb on + strand, within curA at 172.511 kb on - strand, within curA at 172.512 kb on + strand, within curA at 172.512 kb on + strand, within curA at 172.512 kb on + strand, within curA at 172.524 kb on + strand, within curA at 172.524 kb on + strand, within curA at 172.524 kb on + strand, within curA at 172.543 kb on + strand, within curA at 172.544 kb on - strand, within curA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 170,220 + -0.8 170,270 - +0.6 170,328 - -0.5 170,349 - -0.4 170,456 - +0.4 170,456 - -0.0 170,456 - +1.1 170,456 - +0.4 170,456 - -0.4 170,490 - -0.3 170,495 + +1.0 170,496 - -0.6 170,498 + +0.6 170,498 + +0.0 170,499 - +0.1 170,499 - -0.1 170,548 - +0.2 170,554 - -1.9 170,554 - +0.3 170,556 - +0.1 170,556 - +0.8 170,556 - -0.4 170,559 - +0.4 170,583 + -1.7 170,927 + -1.1 171,051 + ydcY MCAODC_16395 0.32 +2.1 171,098 + ydcY MCAODC_16395 0.53 +0.4 171,151 + ydcY MCAODC_16395 0.75 -1.8 171,151 + ydcY MCAODC_16395 0.75 -0.3 171,151 + ydcY MCAODC_16395 0.75 +1.1 171,151 + ydcY MCAODC_16395 0.75 +0.4 171,152 - ydcY MCAODC_16395 0.76 +0.8 171,361 + MCAODC_16400 0.34 -1.9 171,362 - MCAODC_16400 0.34 -2.8 171,435 - MCAODC_16400 0.50 -0.5 171,437 - MCAODC_16400 0.51 +2.8 171,437 - MCAODC_16400 0.51 +0.4 171,437 - MCAODC_16400 0.51 -0.6 171,485 - MCAODC_16400 0.61 +1.0 171,703 - +1.0 172,009 + mddA MCAODC_16405 0.68 -0.3 172,031 - mddA MCAODC_16405 0.72 +0.9 172,102 - mddA MCAODC_16405 0.86 +0.8 172,103 + mddA MCAODC_16405 0.86 +0.1 172,103 + mddA MCAODC_16405 0.86 -1.7 172,192 - -2.2 172,192 - +0.8 172,192 - +0.4 172,212 + -0.7 172,212 + -0.1 172,318 - +1.1 172,479 - curA MCAODC_16410 0.12 -2.1 172,510 + curA MCAODC_16410 0.15 +0.2 172,510 + curA MCAODC_16410 0.15 +1.0 172,511 - curA MCAODC_16410 0.15 -0.2 172,512 + curA MCAODC_16410 0.15 -2.8 172,512 + curA MCAODC_16410 0.15 +0.4 172,512 + curA MCAODC_16410 0.15 -0.8 172,524 + curA MCAODC_16410 0.16 +1.1 172,524 + curA MCAODC_16410 0.16 +1.6 172,524 + curA MCAODC_16410 0.16 -0.9 172,543 + curA MCAODC_16410 0.18 +0.5 172,544 - curA MCAODC_16410 0.18 -0.3
Or see this region's nucleotide sequence