Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycjM and ycjN are separated by 13 nucleotides ycjN and MCAODC_15900 are separated by 48 nucleotides MCAODC_15900 and ugpA are separated by 20 nucleotides ugpA and ycjP overlap by 14 nucleotides
MCAODC_15890: ycjM - Glucosylglycerate phosphorylase, at 68,394 to 69,698
ycjM
MCAODC_15895: ycjN - Putative ABC transporter periplasmic-binding protein YcjN, at 69,712 to 70,341
ycjN
MCAODC_15900: MCAODC_15900 - ABC transporter substrate-binding protein, at 70,390 to 71,004
_15900
MCAODC_15905: ugpA - sugar ABC transporter permease, at 71,025 to 71,906
ugpA
MCAODC_15910: ycjP - Inner membrane ABC transporter permease protein YcjP, at 71,893 to 72,735
ycjP
Position (kb) 70
71
72 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 69.598 kb on + strand at 69.797 kb on + strand, within ycjN at 69.994 kb on + strand, within ycjN at 70.088 kb on + strand, within ycjN at 70.340 kb on - strand at 70.505 kb on - strand, within MCAODC_15900 at 70.513 kb on + strand, within MCAODC_15900 at 70.513 kb on + strand, within MCAODC_15900 at 70.513 kb on + strand, within MCAODC_15900 at 70.513 kb on + strand, within MCAODC_15900 at 70.513 kb on + strand, within MCAODC_15900 at 70.513 kb on + strand, within MCAODC_15900 at 70.513 kb on + strand, within MCAODC_15900 at 70.514 kb on - strand, within MCAODC_15900 at 70.514 kb on - strand, within MCAODC_15900 at 70.515 kb on + strand, within MCAODC_15900 at 70.785 kb on + strand, within MCAODC_15900 at 70.833 kb on + strand, within MCAODC_15900 at 70.920 kb on + strand, within MCAODC_15900 at 70.931 kb on + strand, within MCAODC_15900 at 71.089 kb on + strand at 71.090 kb on - strand at 71.093 kb on - strand at 71.093 kb on - strand at 71.202 kb on + strand, within ugpA at 71.202 kb on + strand, within ugpA at 71.202 kb on + strand, within ugpA at 71.202 kb on + strand, within ugpA at 71.202 kb on + strand, within ugpA at 71.203 kb on - strand, within ugpA at 71.211 kb on - strand, within ugpA at 71.260 kb on - strand, within ugpA at 71.370 kb on + strand, within ugpA at 71.374 kb on + strand, within ugpA at 71.384 kb on + strand, within ugpA at 71.409 kb on + strand, within ugpA at 71.409 kb on + strand, within ugpA at 71.470 kb on - strand, within ugpA at 71.721 kb on + strand, within ugpA at 71.721 kb on + strand, within ugpA at 71.722 kb on - strand, within ugpA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 69,598 + -0.1 69,797 + ycjN MCAODC_15895 0.13 +0.9 69,994 + ycjN MCAODC_15895 0.45 +0.2 70,088 + ycjN MCAODC_15895 0.60 +0.5 70,340 - -2.0 70,505 - MCAODC_15900 0.19 -0.0 70,513 + MCAODC_15900 0.20 -0.3 70,513 + MCAODC_15900 0.20 -0.6 70,513 + MCAODC_15900 0.20 -0.4 70,513 + MCAODC_15900 0.20 -0.5 70,513 + MCAODC_15900 0.20 +0.0 70,513 + MCAODC_15900 0.20 -1.7 70,513 + MCAODC_15900 0.20 -0.6 70,514 - MCAODC_15900 0.20 +1.1 70,514 - MCAODC_15900 0.20 +1.1 70,515 + MCAODC_15900 0.20 -1.9 70,785 + MCAODC_15900 0.64 +1.4 70,833 + MCAODC_15900 0.72 +0.4 70,920 + MCAODC_15900 0.86 +0.7 70,931 + MCAODC_15900 0.88 +1.3 71,089 + +0.8 71,090 - +1.5 71,093 - +1.3 71,093 - -1.2 71,202 + ugpA MCAODC_15905 0.20 -2.8 71,202 + ugpA MCAODC_15905 0.20 +1.2 71,202 + ugpA MCAODC_15905 0.20 -1.9 71,202 + ugpA MCAODC_15905 0.20 +1.5 71,202 + ugpA MCAODC_15905 0.20 +0.4 71,203 - ugpA MCAODC_15905 0.20 -1.7 71,211 - ugpA MCAODC_15905 0.21 +0.8 71,260 - ugpA MCAODC_15905 0.27 +0.8 71,370 + ugpA MCAODC_15905 0.39 -0.2 71,374 + ugpA MCAODC_15905 0.40 +0.3 71,384 + ugpA MCAODC_15905 0.41 +0.4 71,409 + ugpA MCAODC_15905 0.44 +0.2 71,409 + ugpA MCAODC_15905 0.44 -1.4 71,470 - ugpA MCAODC_15905 0.50 +0.0 71,721 + ugpA MCAODC_15905 0.79 +0.7 71,721 + ugpA MCAODC_15905 0.79 -2.7 71,722 - ugpA MCAODC_15905 0.79 +0.1
Or see this region's nucleotide sequence