Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fruB and fruB overlap by 1 nucleotides fruB and fruA are separated by 16 nucleotides
MCAODC_14485: fruB - fused PTS fructose transporter subunit IIA/HPr protein, at 2,849,296 to 2,850,426
fruB
MCAODC_14490: fruB - fused PTS fructose transporter subunit IIA/HPr protein, at 2,850,426 to 2,851,364
fruB
MCAODC_14495: fruA - PTS fructose transporter subunit IIBC, at 2,851,381 to 2,853,072
fruA
Position (kb)
2850
2851
2852 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2849.467 kb on - strand, within fruB at 2849.575 kb on + strand, within fruB at 2849.575 kb on + strand, within fruB at 2849.576 kb on - strand, within fruB at 2849.579 kb on + strand, within fruB at 2849.579 kb on + strand, within fruB at 2850.039 kb on + strand, within fruB at 2850.039 kb on + strand, within fruB at 2850.225 kb on + strand, within fruB at 2850.243 kb on + strand, within fruB at 2850.243 kb on + strand, within fruB at 2850.443 kb on + strand at 2850.443 kb on + strand at 2850.443 kb on + strand at 2850.443 kb on + strand at 2850.443 kb on + strand at 2850.446 kb on + strand at 2850.446 kb on + strand at 2850.465 kb on + strand at 2850.682 kb on + strand, within fruB at 2850.683 kb on - strand, within fruB at 2850.701 kb on + strand, within fruB at 2850.927 kb on - strand, within fruB at 2850.946 kb on + strand, within fruB at 2850.947 kb on - strand, within fruB at 2851.073 kb on + strand, within fruB at 2851.088 kb on + strand, within fruB at 2851.088 kb on + strand, within fruB at 2851.193 kb on + strand, within fruB at 2851.193 kb on + strand, within fruB at 2851.222 kb on + strand, within fruB at 2851.301 kb on + strand at 2851.301 kb on + strand at 2851.348 kb on + strand at 2851.379 kb on + strand at 2851.429 kb on + strand at 2851.429 kb on + strand at 2851.429 kb on + strand at 2851.429 kb on + strand at 2851.429 kb on + strand at 2851.429 kb on + strand at 2851.429 kb on + strand at 2851.429 kb on + strand at 2851.430 kb on - strand at 2851.430 kb on - strand at 2851.642 kb on + strand, within fruA at 2851.643 kb on - strand, within fruA at 2851.643 kb on - strand, within fruA at 2851.697 kb on + strand, within fruA at 2851.727 kb on + strand, within fruA at 2851.740 kb on + strand, within fruA at 2851.882 kb on - strand, within fruA at 2851.922 kb on - strand, within fruA at 2851.997 kb on - strand, within fruA at 2852.086 kb on + strand, within fruA at 2852.086 kb on + strand, within fruA at 2852.086 kb on + strand, within fruA at 2852.086 kb on + strand, within fruA at 2852.086 kb on + strand, within fruA at 2852.086 kb on + strand, within fruA at 2852.087 kb on - strand, within fruA at 2852.087 kb on - strand, within fruA at 2852.243 kb on - strand, within fruA at 2852.244 kb on + strand, within fruA at 2852.363 kb on - strand, within fruA at 2852.363 kb on - strand, within fruA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,849,467 - fruB MCAODC_14485 0.15 +1.8 2,849,575 + fruB MCAODC_14485 0.25 +2.3 2,849,575 + fruB MCAODC_14485 0.25 +0.1 2,849,576 - fruB MCAODC_14485 0.25 +1.0 2,849,579 + fruB MCAODC_14485 0.25 +0.8 2,849,579 + fruB MCAODC_14485 0.25 +0.2 2,850,039 + fruB MCAODC_14485 0.66 -0.2 2,850,039 + fruB MCAODC_14485 0.66 +0.6 2,850,225 + fruB MCAODC_14485 0.82 +1.2 2,850,243 + fruB MCAODC_14485 0.84 +0.3 2,850,243 + fruB MCAODC_14485 0.84 -0.1 2,850,443 + -1.2 2,850,443 + -2.2 2,850,443 + -1.4 2,850,443 + -1.4 2,850,443 + -1.1 2,850,446 + -4.2 2,850,446 + -0.1 2,850,465 + -0.4 2,850,682 + fruB MCAODC_14490 0.27 -1.3 2,850,683 - fruB MCAODC_14490 0.27 -0.6 2,850,701 + fruB MCAODC_14490 0.29 -1.7 2,850,927 - fruB MCAODC_14490 0.53 -1.4 2,850,946 + fruB MCAODC_14490 0.55 -2.0 2,850,947 - fruB MCAODC_14490 0.55 -1.9 2,851,073 + fruB MCAODC_14490 0.69 +0.0 2,851,088 + fruB MCAODC_14490 0.71 -2.3 2,851,088 + fruB MCAODC_14490 0.71 +0.5 2,851,193 + fruB MCAODC_14490 0.82 -2.5 2,851,193 + fruB MCAODC_14490 0.82 -0.3 2,851,222 + fruB MCAODC_14490 0.85 -1.2 2,851,301 + -1.5 2,851,301 + -0.8 2,851,348 + +0.5 2,851,379 + -0.0 2,851,429 + +0.6 2,851,429 + -0.3 2,851,429 + +0.8 2,851,429 + +0.8 2,851,429 + +0.7 2,851,429 + -1.7 2,851,429 + -1.4 2,851,429 + +0.4 2,851,430 - -0.6 2,851,430 - +0.8 2,851,642 + fruA MCAODC_14495 0.15 +0.7 2,851,643 - fruA MCAODC_14495 0.15 +0.3 2,851,643 - fruA MCAODC_14495 0.15 -1.3 2,851,697 + fruA MCAODC_14495 0.19 -1.8 2,851,727 + fruA MCAODC_14495 0.20 +0.4 2,851,740 + fruA MCAODC_14495 0.21 +0.1 2,851,882 - fruA MCAODC_14495 0.30 +0.6 2,851,922 - fruA MCAODC_14495 0.32 +3.4 2,851,997 - fruA MCAODC_14495 0.36 +0.0 2,852,086 + fruA MCAODC_14495 0.42 +0.5 2,852,086 + fruA MCAODC_14495 0.42 +1.8 2,852,086 + fruA MCAODC_14495 0.42 +0.2 2,852,086 + fruA MCAODC_14495 0.42 +0.5 2,852,086 + fruA MCAODC_14495 0.42 -0.3 2,852,086 + fruA MCAODC_14495 0.42 -0.2 2,852,087 - fruA MCAODC_14495 0.42 +0.4 2,852,087 - fruA MCAODC_14495 0.42 -0.7 2,852,243 - fruA MCAODC_14495 0.51 -0.7 2,852,244 + fruA MCAODC_14495 0.51 +0.4 2,852,363 - fruA MCAODC_14495 0.58 -1.5 2,852,363 - fruA MCAODC_14495 0.58 +2.3
Or see this region's nucleotide sequence