Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dapE and yffB are separated by 3 nucleotides yffB and ypfM are separated by 380 nucleotides ypfM and acrD are separated by 98 nucleotides
MCAODC_12485: dapE - succinyl-diaminopimelate desuccinylase, at 2,476,107 to 2,477,234
dapE
MCAODC_12490: yffB - ArsC family reductase, at 2,477,238 to 2,477,594
yffB
MCAODC_12495: ypfM - protein YpfM, at 2,477,975 to 2,478,034
ypfM
MCAODC_12500: acrD - multidrug efflux RND transporter permease AcrD, at 2,478,133 to 2,481,246
acrD
Position (kb)
2477
2478
2479 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2477.379 kb on + strand, within yffB at 2477.379 kb on + strand, within yffB at 2477.380 kb on - strand, within yffB at 2477.417 kb on + strand, within yffB at 2477.418 kb on - strand, within yffB at 2477.418 kb on - strand, within yffB at 2477.449 kb on - strand, within yffB at 2477.459 kb on + strand, within yffB at 2477.460 kb on - strand, within yffB at 2477.676 kb on - strand at 2477.691 kb on + strand at 2477.707 kb on + strand at 2477.742 kb on + strand at 2477.793 kb on + strand at 2477.793 kb on + strand at 2477.796 kb on + strand at 2477.796 kb on + strand at 2477.806 kb on - strand at 2477.811 kb on + strand at 2477.812 kb on - strand at 2477.861 kb on + strand at 2477.861 kb on + strand at 2477.861 kb on + strand at 2477.862 kb on - strand at 2477.864 kb on + strand at 2477.864 kb on + strand at 2477.865 kb on - strand at 2477.865 kb on - strand at 2477.868 kb on + strand at 2477.868 kb on + strand at 2477.869 kb on - strand at 2477.872 kb on + strand at 2477.872 kb on + strand at 2477.873 kb on - strand at 2477.892 kb on + strand at 2477.917 kb on + strand at 2477.917 kb on + strand at 2477.931 kb on + strand at 2477.932 kb on - strand at 2477.933 kb on + strand at 2477.934 kb on - strand at 2477.934 kb on - strand at 2477.955 kb on + strand at 2477.956 kb on - strand at 2477.959 kb on + strand at 2477.959 kb on + strand at 2477.959 kb on + strand at 2477.960 kb on - strand at 2477.961 kb on + strand at 2477.962 kb on - strand at 2477.974 kb on + strand at 2478.020 kb on - strand, within ypfM at 2478.028 kb on + strand, within ypfM at 2478.028 kb on + strand, within ypfM at 2478.029 kb on - strand at 2478.029 kb on - strand at 2478.049 kb on + strand at 2478.050 kb on - strand at 2478.135 kb on - strand at 2478.197 kb on + strand at 2478.197 kb on + strand at 2478.198 kb on - strand at 2478.198 kb on - strand at 2478.309 kb on + strand at 2478.309 kb on + strand at 2478.310 kb on - strand at 2478.341 kb on - strand at 2478.476 kb on + strand, within acrD at 2478.578 kb on + strand, within acrD at 2478.578 kb on + strand, within acrD at 2478.578 kb on + strand, within acrD at 2478.579 kb on - strand, within acrD at 2478.601 kb on + strand, within acrD at 2478.726 kb on + strand, within acrD at 2478.797 kb on + strand, within acrD at 2478.798 kb on - strand, within acrD at 2478.874 kb on - strand, within acrD at 2478.884 kb on + strand, within acrD at 2478.884 kb on + strand, within acrD at 2478.938 kb on + strand, within acrD at 2478.980 kb on + strand, within acrD at 2478.980 kb on + strand, within acrD at 2478.981 kb on - strand, within acrD at 2479.014 kb on + strand, within acrD at 2479.017 kb on + strand, within acrD
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,477,379 + yffB MCAODC_12490 0.39 +0.3 2,477,379 + yffB MCAODC_12490 0.39 +0.3 2,477,380 - yffB MCAODC_12490 0.40 +0.3 2,477,417 + yffB MCAODC_12490 0.50 +1.3 2,477,418 - yffB MCAODC_12490 0.50 -0.5 2,477,418 - yffB MCAODC_12490 0.50 +0.0 2,477,449 - yffB MCAODC_12490 0.59 -1.3 2,477,459 + yffB MCAODC_12490 0.62 +1.8 2,477,460 - yffB MCAODC_12490 0.62 -3.8 2,477,676 - +0.5 2,477,691 + +1.1 2,477,707 + -2.2 2,477,742 + -2.2 2,477,793 + +1.2 2,477,793 + +1.0 2,477,796 + +0.4 2,477,796 + +0.7 2,477,806 - -1.1 2,477,811 + +0.1 2,477,812 - +0.5 2,477,861 + -1.3 2,477,861 + +1.1 2,477,861 + -0.6 2,477,862 - +0.8 2,477,864 + -0.4 2,477,864 + +0.2 2,477,865 - +0.2 2,477,865 - -0.4 2,477,868 + +0.5 2,477,868 + +0.7 2,477,869 - +0.2 2,477,872 + -2.1 2,477,872 + -0.1 2,477,873 - -0.0 2,477,892 + +0.2 2,477,917 + +1.3 2,477,917 + +0.0 2,477,931 + -0.5 2,477,932 - +0.5 2,477,933 + -0.5 2,477,934 - +0.5 2,477,934 - -0.5 2,477,955 + +1.6 2,477,956 - +0.9 2,477,959 + +0.6 2,477,959 + -0.2 2,477,959 + +0.9 2,477,960 - +0.7 2,477,961 + +0.5 2,477,962 - +1.6 2,477,974 + -1.2 2,478,020 - ypfM MCAODC_12495 0.75 -0.4 2,478,028 + ypfM MCAODC_12495 0.88 +0.7 2,478,028 + ypfM MCAODC_12495 0.88 +1.2 2,478,029 - -0.3 2,478,029 - +1.7 2,478,049 + +0.9 2,478,050 - +0.6 2,478,135 - +1.2 2,478,197 + -1.5 2,478,197 + +1.5 2,478,198 - +0.4 2,478,198 - +0.1 2,478,309 + +2.0 2,478,309 + -0.2 2,478,310 - +0.6 2,478,341 - +0.6 2,478,476 + acrD MCAODC_12500 0.11 -1.1 2,478,578 + acrD MCAODC_12500 0.14 -2.2 2,478,578 + acrD MCAODC_12500 0.14 -1.3 2,478,578 + acrD MCAODC_12500 0.14 +0.1 2,478,579 - acrD MCAODC_12500 0.14 -0.1 2,478,601 + acrD MCAODC_12500 0.15 -0.8 2,478,726 + acrD MCAODC_12500 0.19 +0.7 2,478,797 + acrD MCAODC_12500 0.21 -0.3 2,478,798 - acrD MCAODC_12500 0.21 -2.2 2,478,874 - acrD MCAODC_12500 0.24 +1.0 2,478,884 + acrD MCAODC_12500 0.24 -0.3 2,478,884 + acrD MCAODC_12500 0.24 -0.4 2,478,938 + acrD MCAODC_12500 0.26 -0.1 2,478,980 + acrD MCAODC_12500 0.27 -0.2 2,478,980 + acrD MCAODC_12500 0.27 +1.1 2,478,981 - acrD MCAODC_12500 0.27 +0.4 2,479,014 + acrD MCAODC_12500 0.28 +0.8 2,479,017 + acrD MCAODC_12500 0.28 +0.5
Or see this region's nucleotide sequence