Strain Fitness in Escherichia coli ECRC101 around MCAODC_11315

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntemrA and emrR are separated by 126 nucleotidesemrR and ygaH are separated by 90 nucleotidesygaH and ygaZ overlap by 11 nucleotidesygaZ and ygaY are separated by 123 nucleotides MCAODC_11305: emrA - multidrug efflux MFS transporter periplasmic adaptor subunit EmrA, at 2,248,201 to 2,249,373 emrA MCAODC_11310: emrR - multidrug efflux transporter EmrAB transcriptional repressor EmrR, at 2,249,500 to 2,250,030 emrR MCAODC_11315: ygaH - L-valine transporter subunit YgaH, at 2,250,121 to 2,250,456 ygaH MCAODC_11320: ygaZ - L-valine exporter subunit YgaZ, at 2,250,446 to 2,251,183 ygaZ MCAODC_11325: ygaY - Putative uncharacterized transporter YgaY, at 2,251,307 to 2,252,491 ygaY Position (kb) 2250 2251Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 2249.139 kb on + strand, within emrAat 2249.182 kb on - strand, within emrAat 2249.182 kb on - strand, within emrAat 2249.182 kb on - strand, within emrAat 2249.208 kb on - strand, within emrAat 2249.208 kb on - strand, within emrAat 2249.253 kb on - strand, within emrAat 2249.275 kb on - strandat 2249.278 kb on + strandat 2249.313 kb on + strandat 2249.314 kb on - strandat 2249.314 kb on - strandat 2249.374 kb on - strandat 2249.374 kb on - strandat 2249.394 kb on + strandat 2249.394 kb on + strandat 2249.394 kb on + strandat 2249.395 kb on - strandat 2249.469 kb on - strandat 2249.470 kb on + strandat 2249.470 kb on + strandat 2249.471 kb on - strandat 2249.471 kb on - strandat 2249.632 kb on - strand, within emrRat 2249.645 kb on - strand, within emrRat 2249.761 kb on - strand, within emrRat 2249.761 kb on - strand, within emrRat 2249.801 kb on - strand, within emrRat 2249.926 kb on - strand, within emrRat 2249.926 kb on - strand, within emrRat 2250.024 kb on + strandat 2250.025 kb on - strandat 2250.087 kb on - strandat 2250.092 kb on + strandat 2250.092 kb on + strandat 2250.093 kb on - strandat 2250.122 kb on + strandat 2250.122 kb on + strandat 2250.122 kb on + strandat 2250.124 kb on + strandat 2250.124 kb on + strandat 2250.124 kb on + strandat 2250.125 kb on - strandat 2250.125 kb on - strandat 2250.125 kb on - strandat 2250.125 kb on - strandat 2250.126 kb on + strandat 2250.126 kb on + strandat 2250.126 kb on + strandat 2250.127 kb on - strandat 2250.156 kb on - strand, within ygaHat 2250.169 kb on - strand, within ygaHat 2250.169 kb on - strand, within ygaHat 2250.201 kb on + strand, within ygaHat 2250.203 kb on + strand, within ygaHat 2250.340 kb on + strand, within ygaHat 2250.341 kb on - strand, within ygaHat 2250.395 kb on + strand, within ygaHat 2250.407 kb on + strand, within ygaHat 2250.425 kb on - strandat 2250.449 kb on + strandat 2250.450 kb on - strandat 2250.485 kb on + strandat 2250.543 kb on - strand, within ygaZat 2250.646 kb on + strand, within ygaZat 2250.711 kb on - strand, within ygaZat 2250.718 kb on + strand, within ygaZat 2250.726 kb on - strand, within ygaZat 2250.881 kb on - strand, within ygaZat 2250.881 kb on - strand, within ygaZat 2250.884 kb on - strand, within ygaZat 2250.884 kb on - strand, within ygaZat 2250.961 kb on + strand, within ygaZat 2251.011 kb on + strand, within ygaZat 2251.011 kb on + strand, within ygaZat 2251.011 kb on + strand, within ygaZat 2251.097 kb on - strand, within ygaZat 2251.097 kb on - strand, within ygaZat 2251.098 kb on + strand, within ygaZat 2251.098 kb on + strand, within ygaZat 2251.099 kb on - strand, within ygaZat 2251.099 kb on - strand, within ygaZat 2251.099 kb on - strand, within ygaZat 2251.102 kb on + strand, within ygaZat 2251.102 kb on + strand, within ygaZat 2251.102 kb on + strand, within ygaZat 2251.102 kb on + strand, within ygaZat 2251.102 kb on + strand, within ygaZat 2251.103 kb on - strand, within ygaZat 2251.105 kb on + strand, within ygaZat 2251.105 kb on + strand, within ygaZat 2251.106 kb on - strand, within ygaZat 2251.115 kb on + strandat 2251.190 kb on + strandat 2251.190 kb on + strandat 2251.191 kb on - strandat 2251.191 kb on - strandat 2251.209 kb on + strandat 2251.209 kb on + strandat 2251.212 kb on + strandat 2251.212 kb on + strandat 2251.215 kb on - strandat 2251.215 kb on - strandat 2251.218 kb on + strandat 2251.237 kb on - strandat 2251.242 kb on + strandat 2251.263 kb on - strandat 2251.272 kb on - strandat 2251.273 kb on + strandat 2251.273 kb on + strandat 2251.273 kb on + strandat 2251.274 kb on - strandat 2251.274 kb on - strandat 2251.274 kb on - strandat 2251.274 kb on - strandat 2251.274 kb on - strandat 2251.279 kb on + strandat 2251.308 kb on + strandat 2251.308 kb on + strandat 2251.352 kb on + strandat 2251.352 kb on + strandat 2251.352 kb on + strandat 2251.353 kb on - strandat 2251.353 kb on - strandat 2251.353 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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2,249,139 + emrA MCAODC_11305 0.80 -0.3
2,249,182 - emrA MCAODC_11305 0.84 -0.8
2,249,182 - emrA MCAODC_11305 0.84 +0.9
2,249,182 - emrA MCAODC_11305 0.84 -0.7
2,249,208 - emrA MCAODC_11305 0.86 -0.1
2,249,208 - emrA MCAODC_11305 0.86 +0.4
2,249,253 - emrA MCAODC_11305 0.90 -1.1
2,249,275 - +0.6
2,249,278 + +0.5
2,249,313 + +0.9
2,249,314 - -0.0
2,249,314 - -2.1
2,249,374 - -2.8
2,249,374 - -1.3
2,249,394 + +1.7
2,249,394 + -1.8
2,249,394 + +0.7
2,249,395 - -0.3
2,249,469 - -0.9
2,249,470 + +0.1
2,249,470 + -0.8
2,249,471 - -2.1
2,249,471 - -0.7
2,249,632 - emrR MCAODC_11310 0.25 -0.4
2,249,645 - emrR MCAODC_11310 0.27 +0.6
2,249,761 - emrR MCAODC_11310 0.49 +0.7
2,249,761 - emrR MCAODC_11310 0.49 +0.4
2,249,801 - emrR MCAODC_11310 0.57 -0.6
2,249,926 - emrR MCAODC_11310 0.80 +0.2
2,249,926 - emrR MCAODC_11310 0.80 +0.1
2,250,024 + -1.6
2,250,025 - +0.7
2,250,087 - +0.8
2,250,092 + -0.8
2,250,092 + -4.4
2,250,093 - +0.2
2,250,122 + -1.3
2,250,122 + +0.0
2,250,122 + +1.4
2,250,124 + -2.5
2,250,124 + -0.1
2,250,124 + -1.1
2,250,125 - +0.2
2,250,125 - +2.0
2,250,125 - -0.4
2,250,125 - +1.0
2,250,126 + -1.1
2,250,126 + -2.7
2,250,126 + +0.1
2,250,127 - -1.4
2,250,156 - ygaH MCAODC_11315 0.10 +0.5
2,250,169 - ygaH MCAODC_11315 0.14 +0.0
2,250,169 - ygaH MCAODC_11315 0.14 +0.4
2,250,201 + ygaH MCAODC_11315 0.24 -0.0
2,250,203 + ygaH MCAODC_11315 0.24 +1.0
2,250,340 + ygaH MCAODC_11315 0.65 -0.6
2,250,341 - ygaH MCAODC_11315 0.65 -1.1
2,250,395 + ygaH MCAODC_11315 0.82 +0.4
2,250,407 + ygaH MCAODC_11315 0.85 +0.2
2,250,425 - +0.1
2,250,449 + +1.7
2,250,450 - +1.3
2,250,485 + -0.5
2,250,543 - ygaZ MCAODC_11320 0.13 -1.6
2,250,646 + ygaZ MCAODC_11320 0.27 -1.1
2,250,711 - ygaZ MCAODC_11320 0.36 -0.9
2,250,718 + ygaZ MCAODC_11320 0.37 +2.2
2,250,726 - ygaZ MCAODC_11320 0.38 -1.1
2,250,881 - ygaZ MCAODC_11320 0.59 -0.5
2,250,881 - ygaZ MCAODC_11320 0.59 -0.2
2,250,884 - ygaZ MCAODC_11320 0.59 +0.8
2,250,884 - ygaZ MCAODC_11320 0.59 -0.2
2,250,961 + ygaZ MCAODC_11320 0.70 -0.7
2,251,011 + ygaZ MCAODC_11320 0.77 -1.1
2,251,011 + ygaZ MCAODC_11320 0.77 -0.2
2,251,011 + ygaZ MCAODC_11320 0.77 +0.1
2,251,097 - ygaZ MCAODC_11320 0.88 -0.1
2,251,097 - ygaZ MCAODC_11320 0.88 +0.9
2,251,098 + ygaZ MCAODC_11320 0.88 -1.0
2,251,098 + ygaZ MCAODC_11320 0.88 -0.0
2,251,099 - ygaZ MCAODC_11320 0.88 -0.5
2,251,099 - ygaZ MCAODC_11320 0.88 +0.3
2,251,099 - ygaZ MCAODC_11320 0.88 -0.2
2,251,102 + ygaZ MCAODC_11320 0.89 -0.2
2,251,102 + ygaZ MCAODC_11320 0.89 +0.0
2,251,102 + ygaZ MCAODC_11320 0.89 -1.1
2,251,102 + ygaZ MCAODC_11320 0.89 +1.0
2,251,102 + ygaZ MCAODC_11320 0.89 +0.1
2,251,103 - ygaZ MCAODC_11320 0.89 -1.5
2,251,105 + ygaZ MCAODC_11320 0.89 -1.0
2,251,105 + ygaZ MCAODC_11320 0.89 +0.6
2,251,106 - ygaZ MCAODC_11320 0.89 +0.8
2,251,115 + -0.5
2,251,190 + -0.3
2,251,190 + +0.2
2,251,191 - +0.4
2,251,191 - +1.6
2,251,209 + -1.4
2,251,209 + -0.2
2,251,212 + +1.1
2,251,212 + -2.2
2,251,215 - -0.5
2,251,215 - +0.2
2,251,218 + -3.1
2,251,237 - -0.2
2,251,242 + -0.3
2,251,263 - +1.0
2,251,272 - +1.3
2,251,273 + +0.1
2,251,273 + -0.4
2,251,273 + -1.4
2,251,274 - +0.7
2,251,274 - +0.9
2,251,274 - -0.1
2,251,274 - +0.5
2,251,274 - +0.2
2,251,279 + -0.6
2,251,308 + -4.1
2,251,308 + -0.1
2,251,352 + +0.0
2,251,352 + -0.1
2,251,352 + +0.9
2,251,353 - +0.6
2,251,353 - -0.0
2,251,353 - +0.9

Or see this region's nucleotide sequence