Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hypC and hypB overlap by 10 nucleotides hypB and hypA are separated by 3 nucleotides hypA and hycA are separated by 211 nucleotides hycA and hycB are separated by 124 nucleotides
MCAODC_11080: hypC - hydrogenase 3 maturation protein HypC, at 2,208,929 to 2,209,201
hypC
MCAODC_11085: hypB - hydrogenase nickel incorporation protein HypB, at 2,209,192 to 2,210,064
hypB
MCAODC_11090: hypA - hydrogenase maturation nickel metallochaperone HypA, at 2,210,068 to 2,210,418
hypA
MCAODC_11095: hycA - formate hydrogenlyase regulator HycA, at 2,210,630 to 2,211,091
hycA
MCAODC_11100: hycB - formate hydrogenlyase subunit HycB, at 2,211,216 to 2,211,827
hycB
Position (kb)
2210
2211 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2209.194 kb on - strand at 2209.260 kb on + strand at 2209.384 kb on - strand, within hypB at 2209.474 kb on + strand, within hypB at 2209.691 kb on - strand, within hypB at 2209.887 kb on - strand, within hypB at 2209.936 kb on - strand, within hypB at 2210.008 kb on - strand at 2210.028 kb on - strand at 2210.028 kb on - strand at 2210.058 kb on + strand at 2210.059 kb on - strand at 2210.080 kb on + strand at 2210.081 kb on - strand at 2210.125 kb on + strand, within hypA at 2210.126 kb on - strand, within hypA at 2210.126 kb on - strand, within hypA at 2210.126 kb on - strand, within hypA at 2210.126 kb on - strand, within hypA at 2210.183 kb on + strand, within hypA at 2210.183 kb on + strand, within hypA at 2210.184 kb on - strand, within hypA at 2210.184 kb on - strand, within hypA at 2210.184 kb on - strand, within hypA at 2210.184 kb on - strand, within hypA at 2210.184 kb on - strand, within hypA at 2210.238 kb on + strand, within hypA at 2210.407 kb on + strand at 2210.407 kb on + strand at 2210.419 kb on - strand at 2210.431 kb on + strand at 2210.435 kb on + strand at 2210.436 kb on - strand at 2210.436 kb on - strand at 2210.488 kb on + strand at 2210.489 kb on - strand at 2210.561 kb on - strand at 2210.635 kb on + strand at 2210.635 kb on + strand at 2210.635 kb on + strand at 2210.636 kb on - strand at 2210.646 kb on + strand at 2210.646 kb on + strand at 2210.647 kb on - strand at 2210.708 kb on + strand, within hycA at 2210.709 kb on - strand, within hycA at 2210.709 kb on - strand, within hycA at 2210.822 kb on + strand, within hycA at 2210.822 kb on + strand, within hycA at 2210.822 kb on + strand, within hycA at 2210.823 kb on - strand, within hycA at 2210.823 kb on - strand, within hycA at 2210.823 kb on - strand, within hycA at 2210.864 kb on + strand, within hycA at 2210.864 kb on + strand, within hycA at 2210.864 kb on + strand, within hycA at 2210.865 kb on - strand, within hycA at 2210.867 kb on + strand, within hycA at 2210.867 kb on + strand, within hycA at 2210.867 kb on + strand, within hycA at 2210.984 kb on + strand, within hycA at 2210.984 kb on + strand, within hycA at 2210.984 kb on + strand, within hycA at 2210.985 kb on - strand, within hycA at 2211.018 kb on + strand, within hycA at 2211.018 kb on + strand, within hycA at 2211.018 kb on + strand, within hycA at 2211.018 kb on + strand, within hycA at 2211.018 kb on + strand, within hycA at 2211.018 kb on + strand, within hycA at 2211.019 kb on - strand, within hycA at 2211.019 kb on - strand, within hycA at 2211.019 kb on - strand, within hycA at 2211.019 kb on - strand, within hycA at 2211.019 kb on - strand, within hycA at 2211.020 kb on + strand, within hycA at 2211.020 kb on + strand, within hycA at 2211.020 kb on + strand, within hycA at 2211.021 kb on - strand, within hycA at 2211.054 kb on + strand at 2211.121 kb on - strand at 2211.230 kb on - strand at 2211.251 kb on + strand at 2211.252 kb on - strand at 2211.337 kb on + strand, within hycB at 2211.337 kb on + strand, within hycB at 2211.337 kb on + strand, within hycB at 2211.338 kb on - strand, within hycB
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,209,194 - +0.2 2,209,260 + +0.6 2,209,384 - hypB MCAODC_11085 0.22 +0.4 2,209,474 + hypB MCAODC_11085 0.32 -1.0 2,209,691 - hypB MCAODC_11085 0.57 -0.6 2,209,887 - hypB MCAODC_11085 0.80 -0.0 2,209,936 - hypB MCAODC_11085 0.85 -1.3 2,210,008 - -2.2 2,210,028 - -0.3 2,210,028 - -1.2 2,210,058 + +0.2 2,210,059 - +1.3 2,210,080 + +0.9 2,210,081 - -0.4 2,210,125 + hypA MCAODC_11090 0.16 +1.0 2,210,126 - hypA MCAODC_11090 0.17 -0.3 2,210,126 - hypA MCAODC_11090 0.17 -1.9 2,210,126 - hypA MCAODC_11090 0.17 +0.1 2,210,126 - hypA MCAODC_11090 0.17 +0.7 2,210,183 + hypA MCAODC_11090 0.33 +1.2 2,210,183 + hypA MCAODC_11090 0.33 +1.4 2,210,184 - hypA MCAODC_11090 0.33 -1.7 2,210,184 - hypA MCAODC_11090 0.33 +0.4 2,210,184 - hypA MCAODC_11090 0.33 -1.5 2,210,184 - hypA MCAODC_11090 0.33 +0.0 2,210,184 - hypA MCAODC_11090 0.33 -0.3 2,210,238 + hypA MCAODC_11090 0.48 -3.3 2,210,407 + +0.1 2,210,407 + -2.8 2,210,419 - +1.3 2,210,431 + +0.3 2,210,435 + -0.4 2,210,436 - +1.3 2,210,436 - -0.0 2,210,488 + +0.2 2,210,489 - +1.0 2,210,561 - -0.1 2,210,635 + -0.4 2,210,635 + -0.2 2,210,635 + -1.5 2,210,636 - +0.5 2,210,646 + +1.0 2,210,646 + +0.5 2,210,647 - +1.6 2,210,708 + hycA MCAODC_11095 0.17 -0.1 2,210,709 - hycA MCAODC_11095 0.17 -2.0 2,210,709 - hycA MCAODC_11095 0.17 +0.1 2,210,822 + hycA MCAODC_11095 0.42 +0.3 2,210,822 + hycA MCAODC_11095 0.42 -0.4 2,210,822 + hycA MCAODC_11095 0.42 +1.6 2,210,823 - hycA MCAODC_11095 0.42 +0.5 2,210,823 - hycA MCAODC_11095 0.42 -0.7 2,210,823 - hycA MCAODC_11095 0.42 +1.5 2,210,864 + hycA MCAODC_11095 0.51 +0.2 2,210,864 + hycA MCAODC_11095 0.51 +1.2 2,210,864 + hycA MCAODC_11095 0.51 -0.8 2,210,865 - hycA MCAODC_11095 0.51 +1.0 2,210,867 + hycA MCAODC_11095 0.51 +1.2 2,210,867 + hycA MCAODC_11095 0.51 +0.1 2,210,867 + hycA MCAODC_11095 0.51 -0.7 2,210,984 + hycA MCAODC_11095 0.77 -0.2 2,210,984 + hycA MCAODC_11095 0.77 -0.2 2,210,984 + hycA MCAODC_11095 0.77 +0.1 2,210,985 - hycA MCAODC_11095 0.77 -0.0 2,211,018 + hycA MCAODC_11095 0.84 -0.0 2,211,018 + hycA MCAODC_11095 0.84 +0.4 2,211,018 + hycA MCAODC_11095 0.84 -0.0 2,211,018 + hycA MCAODC_11095 0.84 -2.7 2,211,018 + hycA MCAODC_11095 0.84 +0.6 2,211,018 + hycA MCAODC_11095 0.84 +2.0 2,211,019 - hycA MCAODC_11095 0.84 -1.2 2,211,019 - hycA MCAODC_11095 0.84 +0.1 2,211,019 - hycA MCAODC_11095 0.84 +0.0 2,211,019 - hycA MCAODC_11095 0.84 -0.1 2,211,019 - hycA MCAODC_11095 0.84 +2.0 2,211,020 + hycA MCAODC_11095 0.84 +2.1 2,211,020 + hycA MCAODC_11095 0.84 -1.3 2,211,020 + hycA MCAODC_11095 0.84 -2.2 2,211,021 - hycA MCAODC_11095 0.85 +0.1 2,211,054 + -1.4 2,211,121 - -0.8 2,211,230 - -2.8 2,211,251 + -0.0 2,211,252 - +0.4 2,211,337 + hycB MCAODC_11100 0.20 -0.2 2,211,337 + hycB MCAODC_11100 0.20 +0.8 2,211,337 + hycB MCAODC_11100 0.20 -0.4 2,211,338 - hycB MCAODC_11100 0.20 -0.4
Or see this region's nucleotide sequence