Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt recB and recD overlap by 1 nucleotides recD and argA are separated by 61 nucleotides
MCAODC_10575: recB - exodeoxyribonuclease V subunit beta, at 2,106,394 to 2,109,936
recB
MCAODC_10580: recD - exodeoxyribonuclease V subunit alpha, at 2,109,936 to 2,111,762
recD
MCAODC_10585: argA - amino-acid N-acetyltransferase, at 2,111,824 to 2,113,155
argA
Position (kb)
2109
2110
2111
2112 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2109.916 kb on - strand at 2109.934 kb on + strand at 2109.934 kb on + strand at 2109.955 kb on + strand at 2109.982 kb on + strand at 2109.982 kb on + strand at 2110.061 kb on - strand at 2110.061 kb on - strand at 2110.061 kb on - strand at 2110.277 kb on + strand, within recD at 2110.277 kb on + strand, within recD at 2110.498 kb on + strand, within recD at 2110.498 kb on + strand, within recD at 2110.554 kb on - strand, within recD at 2110.632 kb on - strand, within recD at 2110.632 kb on - strand, within recD at 2110.650 kb on - strand, within recD at 2110.738 kb on + strand, within recD at 2110.738 kb on + strand, within recD at 2110.739 kb on - strand, within recD at 2110.739 kb on - strand, within recD at 2110.766 kb on + strand, within recD at 2110.766 kb on + strand, within recD at 2110.767 kb on - strand, within recD at 2110.767 kb on - strand, within recD at 2111.045 kb on - strand, within recD at 2111.111 kb on + strand, within recD at 2111.111 kb on + strand, within recD at 2111.146 kb on - strand, within recD at 2111.146 kb on - strand, within recD at 2111.147 kb on + strand, within recD at 2111.148 kb on - strand, within recD at 2111.248 kb on - strand, within recD at 2111.394 kb on - strand, within recD at 2111.417 kb on + strand, within recD at 2111.490 kb on - strand, within recD at 2111.490 kb on - strand, within recD at 2111.492 kb on + strand, within recD at 2111.531 kb on + strand, within recD at 2111.532 kb on - strand, within recD at 2111.532 kb on - strand, within recD at 2111.532 kb on - strand, within recD at 2111.548 kb on + strand, within recD at 2111.548 kb on + strand, within recD at 2111.549 kb on - strand, within recD at 2111.549 kb on - strand, within recD at 2111.549 kb on - strand, within recD at 2111.552 kb on + strand, within recD at 2111.552 kb on + strand, within recD at 2111.553 kb on - strand, within recD at 2111.618 kb on - strand at 2111.634 kb on + strand at 2111.674 kb on - strand at 2111.689 kb on + strand at 2111.689 kb on + strand at 2111.689 kb on + strand at 2111.690 kb on - strand at 2111.690 kb on - strand at 2111.690 kb on - strand at 2111.692 kb on + strand at 2111.692 kb on + strand at 2111.692 kb on + strand at 2111.692 kb on + strand at 2111.692 kb on + strand at 2111.693 kb on - strand at 2111.693 kb on - strand at 2111.693 kb on - strand at 2111.747 kb on + strand at 2111.748 kb on - strand at 2111.761 kb on - strand at 2111.766 kb on + strand at 2111.766 kb on + strand at 2111.766 kb on + strand at 2111.766 kb on + strand at 2111.767 kb on - strand at 2111.784 kb on + strand at 2111.785 kb on - strand at 2111.793 kb on + strand at 2111.794 kb on - strand at 2111.794 kb on - strand at 2111.856 kb on + strand at 2111.856 kb on + strand at 2111.856 kb on + strand at 2111.857 kb on - strand at 2111.857 kb on - strand at 2111.864 kb on + strand at 2111.864 kb on + strand at 2111.864 kb on + strand at 2111.865 kb on - strand at 2111.865 kb on - strand at 2111.865 kb on - strand at 2111.870 kb on + strand at 2111.870 kb on + strand at 2111.871 kb on - strand at 2111.871 kb on - strand at 2111.943 kb on + strand at 2111.944 kb on - strand at 2112.118 kb on - strand, within argA at 2112.138 kb on + strand, within argA at 2112.138 kb on + strand, within argA at 2112.139 kb on - strand, within argA at 2112.195 kb on + strand, within argA at 2112.195 kb on + strand, within argA at 2112.232 kb on - strand, within argA at 2112.347 kb on + strand, within argA at 2112.347 kb on + strand, within argA at 2112.356 kb on - strand, within argA at 2112.428 kb on + strand, within argA at 2112.428 kb on + strand, within argA at 2112.429 kb on - strand, within argA at 2112.454 kb on + strand, within argA at 2112.496 kb on - strand, within argA at 2112.496 kb on - strand, within argA at 2112.511 kb on - strand, within argA at 2112.600 kb on + strand, within argA at 2112.640 kb on + strand, within argA at 2112.647 kb on - strand, within argA at 2112.647 kb on - strand, within argA at 2112.716 kb on + strand, within argA at 2112.757 kb on - strand, within argA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,109,916 - -1.8 2,109,934 + -1.1 2,109,934 + +0.3 2,109,955 + +0.4 2,109,982 + +0.1 2,109,982 + +1.1 2,110,061 - -2.3 2,110,061 - -0.9 2,110,061 - -0.3 2,110,277 + recD MCAODC_10580 0.19 -2.2 2,110,277 + recD MCAODC_10580 0.19 -0.5 2,110,498 + recD MCAODC_10580 0.31 -2.8 2,110,498 + recD MCAODC_10580 0.31 +0.0 2,110,554 - recD MCAODC_10580 0.34 -2.2 2,110,632 - recD MCAODC_10580 0.38 -2.8 2,110,632 - recD MCAODC_10580 0.38 -1.9 2,110,650 - recD MCAODC_10580 0.39 -2.2 2,110,738 + recD MCAODC_10580 0.44 +0.8 2,110,738 + recD MCAODC_10580 0.44 -2.0 2,110,739 - recD MCAODC_10580 0.44 -0.6 2,110,739 - recD MCAODC_10580 0.44 +0.3 2,110,766 + recD MCAODC_10580 0.45 +1.4 2,110,766 + recD MCAODC_10580 0.45 -1.4 2,110,767 - recD MCAODC_10580 0.45 +0.3 2,110,767 - recD MCAODC_10580 0.45 -0.5 2,111,045 - recD MCAODC_10580 0.61 -0.3 2,111,111 + recD MCAODC_10580 0.64 -0.3 2,111,111 + recD MCAODC_10580 0.64 +0.2 2,111,146 - recD MCAODC_10580 0.66 -1.5 2,111,146 - recD MCAODC_10580 0.66 -0.8 2,111,147 + recD MCAODC_10580 0.66 -0.1 2,111,148 - recD MCAODC_10580 0.66 -0.6 2,111,248 - recD MCAODC_10580 0.72 -3.2 2,111,394 - recD MCAODC_10580 0.80 -1.7 2,111,417 + recD MCAODC_10580 0.81 -2.4 2,111,490 - recD MCAODC_10580 0.85 -1.1 2,111,490 - recD MCAODC_10580 0.85 -0.8 2,111,492 + recD MCAODC_10580 0.85 +0.0 2,111,531 + recD MCAODC_10580 0.87 -2.2 2,111,532 - recD MCAODC_10580 0.87 -2.2 2,111,532 - recD MCAODC_10580 0.87 -1.6 2,111,532 - recD MCAODC_10580 0.87 -0.7 2,111,548 + recD MCAODC_10580 0.88 -2.4 2,111,548 + recD MCAODC_10580 0.88 +0.1 2,111,549 - recD MCAODC_10580 0.88 -1.6 2,111,549 - recD MCAODC_10580 0.88 -3.3 2,111,549 - recD MCAODC_10580 0.88 -1.5 2,111,552 + recD MCAODC_10580 0.88 -0.1 2,111,552 + recD MCAODC_10580 0.88 -0.9 2,111,553 - recD MCAODC_10580 0.89 -0.8 2,111,618 - +0.2 2,111,634 + -0.7 2,111,674 - +0.3 2,111,689 + -0.2 2,111,689 + -0.3 2,111,689 + -1.2 2,111,690 - +0.1 2,111,690 - -1.3 2,111,690 - -1.7 2,111,692 + -0.4 2,111,692 + -0.6 2,111,692 + +0.2 2,111,692 + -0.4 2,111,692 + +0.9 2,111,693 - +1.9 2,111,693 - -0.3 2,111,693 - -0.7 2,111,747 + +0.3 2,111,748 - +0.8 2,111,761 - -2.9 2,111,766 + -0.3 2,111,766 + +1.0 2,111,766 + -1.6 2,111,766 + +0.6 2,111,767 - -0.2 2,111,784 + +0.8 2,111,785 - +0.6 2,111,793 + -0.6 2,111,794 - +0.7 2,111,794 - +0.9 2,111,856 + -0.2 2,111,856 + +1.4 2,111,856 + +0.1 2,111,857 - -0.7 2,111,857 - -0.0 2,111,864 + +0.4 2,111,864 + -0.9 2,111,864 + -1.9 2,111,865 - -1.5 2,111,865 - +0.7 2,111,865 - -0.5 2,111,870 + -3.5 2,111,870 + +0.1 2,111,871 - +0.0 2,111,871 - +2.4 2,111,943 + +0.8 2,111,944 - +0.3 2,112,118 - argA MCAODC_10585 0.22 -1.4 2,112,138 + argA MCAODC_10585 0.24 -1.1 2,112,138 + argA MCAODC_10585 0.24 +0.4 2,112,139 - argA MCAODC_10585 0.24 -0.1 2,112,195 + argA MCAODC_10585 0.28 +2.7 2,112,195 + argA MCAODC_10585 0.28 +1.2 2,112,232 - argA MCAODC_10585 0.31 +1.7 2,112,347 + argA MCAODC_10585 0.39 +1.1 2,112,347 + argA MCAODC_10585 0.39 -0.9 2,112,356 - argA MCAODC_10585 0.40 +0.9 2,112,428 + argA MCAODC_10585 0.45 -2.4 2,112,428 + argA MCAODC_10585 0.45 -0.0 2,112,429 - argA MCAODC_10585 0.45 +1.4 2,112,454 + argA MCAODC_10585 0.47 -0.1 2,112,496 - argA MCAODC_10585 0.50 +0.3 2,112,496 - argA MCAODC_10585 0.50 -2.5 2,112,511 - argA MCAODC_10585 0.52 -0.2 2,112,600 + argA MCAODC_10585 0.58 -0.4 2,112,640 + argA MCAODC_10585 0.61 -3.5 2,112,647 - argA MCAODC_10585 0.62 +0.6 2,112,647 - argA MCAODC_10585 0.62 -0.3 2,112,716 + argA MCAODC_10585 0.67 +1.5 2,112,757 - argA MCAODC_10585 0.70 +0.1
Or see this region's nucleotide sequence