Strain Fitness in Escherichia coli ECRC101 around MCAODC_10265

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMCAODC_10255 and MCAODC_10260 are separated by 66 nucleotidesMCAODC_10260 and araC are separated by 345 nucleotidesaraC and sctC overlap by 4 nucleotides MCAODC_10255: MCAODC_10255 - Glyco-hydro-15 domain-containing protein, at 2,048,264 to 2,049,322 _10255 MCAODC_10260: MCAODC_10260 - GhoT/OrtT family toxin, at 2,049,389 to 2,049,568 _10260 MCAODC_10265: araC - AraC family transcriptional regulator, at 2,049,914 to 2,050,663 araC MCAODC_10270: sctC - type III secretion system outer membrane ring subunit SctC, at 2,050,660 to 2,052,363 sctC Position (kb) 2049 2050 2051Strain fitness (log2 ratio) -2 -1 0 1at 2049.101 kb on - strand, within MCAODC_10255at 2049.194 kb on + strand, within MCAODC_10255at 2049.195 kb on - strand, within MCAODC_10255at 2049.471 kb on + strand, within MCAODC_10260at 2049.713 kb on + strandat 2049.790 kb on + strandat 2049.933 kb on + strandat 2050.177 kb on + strand, within araCat 2050.465 kb on + strand, within araCat 2050.565 kb on - strand, within araCat 2050.763 kb on + strandat 2050.824 kb on + strandat 2050.829 kb on + strandat 2050.829 kb on + strandat 2050.926 kb on + strand, within sctCat 2050.955 kb on - strand, within sctCat 2050.960 kb on + strand, within sctCat 2050.961 kb on - strand, within sctCat 2051.096 kb on - strand, within sctCat 2051.100 kb on - strand, within sctCat 2051.116 kb on + strand, within sctCat 2051.117 kb on - strand, within sctCat 2051.199 kb on - strand, within sctCat 2051.258 kb on + strand, within sctCat 2051.301 kb on + strand, within sctCat 2051.310 kb on + strand, within sctCat 2051.373 kb on - strand, within sctCat 2051.631 kb on - strand, within sctC

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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2,049,101 - MCAODC_10255 0.79 -1.3
2,049,194 + MCAODC_10255 0.88 -0.3
2,049,195 - MCAODC_10255 0.88 -2.3
2,049,471 + MCAODC_10260 0.46 +1.2
2,049,713 + -0.5
2,049,790 + +0.5
2,049,933 + +0.6
2,050,177 + araC MCAODC_10265 0.35 -0.2
2,050,465 + araC MCAODC_10265 0.73 +0.9
2,050,565 - araC MCAODC_10265 0.87 -0.6
2,050,763 + +0.1
2,050,824 + -1.2
2,050,829 + +1.0
2,050,829 + -1.1
2,050,926 + sctC MCAODC_10270 0.16 +0.5
2,050,955 - sctC MCAODC_10270 0.17 -1.2
2,050,960 + sctC MCAODC_10270 0.18 +0.3
2,050,961 - sctC MCAODC_10270 0.18 +1.4
2,051,096 - sctC MCAODC_10270 0.26 +0.3
2,051,100 - sctC MCAODC_10270 0.26 +0.3
2,051,116 + sctC MCAODC_10270 0.27 +1.0
2,051,117 - sctC MCAODC_10270 0.27 +0.6
2,051,199 - sctC MCAODC_10270 0.32 +0.9
2,051,258 + sctC MCAODC_10270 0.35 -0.1
2,051,301 + sctC MCAODC_10270 0.38 -0.5
2,051,310 + sctC MCAODC_10270 0.38 +1.0
2,051,373 - sctC MCAODC_10270 0.42 -1.2
2,051,631 - sctC MCAODC_10270 0.57 +1.4

Or see this region's nucleotide sequence