Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ubiH and ubiI are separated by 23 nucleotides ubiI and gcvT are separated by 447 nucleotides
MCAODC_10030: ubiH - 2-octaprenyl-6-methoxyphenyl hydroxylase, at 1,994,150 to 1,995,328
ubiH
MCAODC_10035: ubiI - FAD-dependent 2-octaprenylphenol hydroxylase, at 1,995,352 to 1,996,554
ubiI
MCAODC_10040: gcvT - glycine cleavage system aminomethyltransferase GcvT, at 1,997,002 to 1,998,096
gcvT
Position (kb) 1995
1996
1997 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 1995.339 kb on + strand at 1995.340 kb on - strand at 1996.575 kb on - strand at 1996.767 kb on + strand at 1996.830 kb on + strand at 1996.835 kb on + strand at 1996.853 kb on + strand at 1996.865 kb on - strand at 1996.884 kb on - strand at 1996.953 kb on - strand at 1997.023 kb on + strand at 1997.023 kb on + strand at 1997.089 kb on + strand at 1997.120 kb on + strand, within gcvT at 1997.120 kb on + strand, within gcvT at 1997.121 kb on - strand, within gcvT at 1997.121 kb on - strand, within gcvT at 1997.121 kb on - strand, within gcvT at 1997.121 kb on - strand, within gcvT at 1997.121 kb on - strand, within gcvT at 1997.121 kb on - strand, within gcvT at 1997.121 kb on - strand, within gcvT at 1997.121 kb on - strand, within gcvT at 1997.121 kb on - strand, within gcvT at 1997.154 kb on + strand, within gcvT at 1997.154 kb on + strand, within gcvT at 1997.154 kb on + strand, within gcvT at 1997.154 kb on + strand, within gcvT at 1997.154 kb on + strand, within gcvT at 1997.155 kb on - strand, within gcvT at 1997.155 kb on - strand, within gcvT at 1997.155 kb on - strand, within gcvT at 1997.155 kb on - strand, within gcvT at 1997.155 kb on - strand, within gcvT at 1997.155 kb on - strand, within gcvT at 1997.255 kb on - strand, within gcvT at 1997.289 kb on - strand, within gcvT at 1997.306 kb on - strand, within gcvT at 1997.309 kb on - strand, within gcvT at 1997.309 kb on - strand, within gcvT at 1997.341 kb on - strand, within gcvT at 1997.341 kb on - strand, within gcvT at 1997.441 kb on + strand, within gcvT at 1997.442 kb on - strand, within gcvT at 1997.481 kb on + strand, within gcvT
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,995,339 + +0.3 1,995,340 - -1.6 1,996,575 - -1.6 1,996,767 + +0.6 1,996,830 + -0.8 1,996,835 + -0.1 1,996,853 + -1.2 1,996,865 - -3.8 1,996,884 - -0.3 1,996,953 - +0.2 1,997,023 + -0.1 1,997,023 + +0.9 1,997,089 + -0.5 1,997,120 + gcvT MCAODC_10040 0.11 +0.1 1,997,120 + gcvT MCAODC_10040 0.11 +0.0 1,997,121 - gcvT MCAODC_10040 0.11 +0.4 1,997,121 - gcvT MCAODC_10040 0.11 +1.0 1,997,121 - gcvT MCAODC_10040 0.11 -0.8 1,997,121 - gcvT MCAODC_10040 0.11 +0.8 1,997,121 - gcvT MCAODC_10040 0.11 -1.7 1,997,121 - gcvT MCAODC_10040 0.11 -0.3 1,997,121 - gcvT MCAODC_10040 0.11 -2.3 1,997,121 - gcvT MCAODC_10040 0.11 +1.5 1,997,121 - gcvT MCAODC_10040 0.11 -0.4 1,997,154 + gcvT MCAODC_10040 0.14 +1.4 1,997,154 + gcvT MCAODC_10040 0.14 +1.3 1,997,154 + gcvT MCAODC_10040 0.14 -0.2 1,997,154 + gcvT MCAODC_10040 0.14 -3.1 1,997,154 + gcvT MCAODC_10040 0.14 +1.5 1,997,155 - gcvT MCAODC_10040 0.14 -0.6 1,997,155 - gcvT MCAODC_10040 0.14 -1.4 1,997,155 - gcvT MCAODC_10040 0.14 -0.1 1,997,155 - gcvT MCAODC_10040 0.14 +0.6 1,997,155 - gcvT MCAODC_10040 0.14 +0.6 1,997,155 - gcvT MCAODC_10040 0.14 -1.2 1,997,255 - gcvT MCAODC_10040 0.23 +0.2 1,997,289 - gcvT MCAODC_10040 0.26 +0.1 1,997,306 - gcvT MCAODC_10040 0.28 +0.6 1,997,309 - gcvT MCAODC_10040 0.28 -1.2 1,997,309 - gcvT MCAODC_10040 0.28 -0.1 1,997,341 - gcvT MCAODC_10040 0.31 -1.4 1,997,341 - gcvT MCAODC_10040 0.31 +0.1 1,997,441 + gcvT MCAODC_10040 0.40 +0.1 1,997,442 - gcvT MCAODC_10040 0.40 -0.2 1,997,481 + gcvT MCAODC_10040 0.44 +0.5
Or see this region's nucleotide sequence