Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ftsH and folP_P64S are separated by 89 nucleotides folP_P64S and glmM overlap by 8 nucleotides glmM and secG are separated by 227 nucleotides secG and Leu_trna are separated by 14 nucleotides
MCAODC_08580: ftsH - ATP-dependent zinc metalloprotease FtsH, at 1,716,753 to 1,718,696
ftsH
MCAODC_08585: folP_P64S - dihydropteroate synthase FolP, at 1,718,786 to 1,719,634
_P64S
MCAODC_08590: glmM - phosphoglucosamine mutase, at 1,719,627 to 1,720,964
glmM
MCAODC_08595: secG - preprotein translocase subunit SecG, at 1,721,192 to 1,721,524
secG
MCAODC_08600: Leu_trna - tRNA-Leu(gag), at 1,721,539 to 1,721,625
_trna
Position (kb)
1719
1720
1721 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1718.694 kb on + strand at 1718.694 kb on + strand at 1718.695 kb on - strand at 1718.717 kb on + strand at 1718.717 kb on + strand at 1718.717 kb on + strand at 1718.717 kb on + strand at 1718.723 kb on + strand at 1718.770 kb on + strand at 1719.598 kb on + strand at 1719.599 kb on - strand at 1720.951 kb on + strand at 1720.951 kb on + strand at 1720.951 kb on + strand at 1720.951 kb on + strand at 1720.962 kb on + strand at 1720.963 kb on - strand at 1720.970 kb on + strand at 1720.971 kb on - strand at 1720.974 kb on + strand at 1721.129 kb on + strand at 1721.142 kb on + strand at 1721.191 kb on + strand at 1721.191 kb on + strand at 1721.191 kb on + strand at 1721.256 kb on + strand, within secG at 1721.696 kb on + strand at 1721.697 kb on - strand at 1721.702 kb on + strand at 1721.703 kb on - strand at 1721.703 kb on - strand at 1721.727 kb on - strand at 1721.733 kb on + strand at 1721.766 kb on + strand at 1721.769 kb on - strand at 1721.791 kb on + strand at 1721.792 kb on - strand at 1721.792 kb on - strand at 1721.803 kb on + strand at 1721.803 kb on + strand at 1721.803 kb on + strand at 1721.879 kb on - strand at 1721.882 kb on + strand at 1721.883 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,718,694 + -2.4 1,718,694 + +2.3 1,718,695 - -0.1 1,718,717 + -0.8 1,718,717 + -2.9 1,718,717 + -1.6 1,718,717 + -1.0 1,718,723 + +0.1 1,718,770 + +0.4 1,719,598 + -0.2 1,719,599 - -1.9 1,720,951 + -0.3 1,720,951 + +0.4 1,720,951 + +1.0 1,720,951 + +0.0 1,720,962 + -0.0 1,720,963 - -0.0 1,720,970 + +0.1 1,720,971 - -0.6 1,720,974 + +0.2 1,721,129 + -0.2 1,721,142 + +0.7 1,721,191 + -1.2 1,721,191 + +1.4 1,721,191 + +1.0 1,721,256 + secG MCAODC_08595 0.19 +0.8 1,721,696 + -1.8 1,721,697 - +0.6 1,721,702 + +0.6 1,721,703 - -0.4 1,721,703 - -0.6 1,721,727 - +2.0 1,721,733 + +0.3 1,721,766 + -1.0 1,721,769 - +0.3 1,721,791 + +1.9 1,721,792 - +1.5 1,721,792 - -0.4 1,721,803 + +0.6 1,721,803 + -1.3 1,721,803 + -0.0 1,721,879 - +0.7 1,721,882 + -1.6 1,721,883 - +2.3
Or see this region's nucleotide sequence