Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mreB and mreC are separated by 65 nucleotides mreC and mreD overlap by 1 nucleotides mreD and yhdE are separated by 8 nucleotides
MCAODC_08245: mreB - rod shape-determining protein MreB, at 1,650,148 to 1,651,191
mreB
MCAODC_08250: mreC - rod shape-determining protein MreC, at 1,651,257 to 1,652,360
mreC
MCAODC_08255: mreD - rod shape-determining protein MreD, at 1,652,360 to 1,652,848
mreD
MCAODC_08260: yhdE - nucleoside triphosphate pyrophosphatase YhdE, at 1,652,857 to 1,653,450
yhdE
Position (kb)
1651
1652
1653 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1651.189 kb on + strand at 1651.189 kb on + strand at 1651.245 kb on + strand at 1652.846 kb on + strand at 1652.846 kb on + strand at 1652.846 kb on + strand at 1652.856 kb on + strand at 1652.857 kb on - strand at 1652.857 kb on - strand at 1652.870 kb on - strand at 1652.870 kb on - strand at 1652.873 kb on + strand at 1652.873 kb on + strand at 1652.874 kb on - strand at 1652.903 kb on + strand at 1652.904 kb on - strand at 1652.934 kb on + strand, within yhdE at 1652.934 kb on + strand, within yhdE at 1652.934 kb on + strand, within yhdE at 1652.934 kb on + strand, within yhdE at 1652.934 kb on + strand, within yhdE at 1652.940 kb on + strand, within yhdE at 1652.986 kb on + strand, within yhdE at 1652.986 kb on + strand, within yhdE at 1652.986 kb on + strand, within yhdE at 1652.986 kb on + strand, within yhdE at 1652.987 kb on - strand, within yhdE at 1652.987 kb on - strand, within yhdE at 1653.070 kb on - strand, within yhdE at 1653.070 kb on - strand, within yhdE at 1653.144 kb on - strand, within yhdE at 1653.291 kb on + strand, within yhdE at 1653.292 kb on - strand, within yhdE at 1653.304 kb on + strand, within yhdE at 1653.305 kb on - strand, within yhdE at 1653.310 kb on - strand, within yhdE at 1653.345 kb on - strand, within yhdE at 1653.355 kb on + strand, within yhdE at 1653.355 kb on + strand, within yhdE at 1653.356 kb on - strand, within yhdE at 1653.356 kb on - strand, within yhdE
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 1,651,189 + +0.3 1,651,189 + +0.4 1,651,245 + -0.3 1,652,846 + -0.8 1,652,846 + +0.4 1,652,846 + +1.4 1,652,856 + +1.0 1,652,857 - -1.3 1,652,857 - +0.5 1,652,870 - -0.6 1,652,870 - -0.7 1,652,873 + +0.4 1,652,873 + -0.5 1,652,874 - -2.8 1,652,903 + -0.3 1,652,904 - -0.2 1,652,934 + yhdE MCAODC_08260 0.13 +0.1 1,652,934 + yhdE MCAODC_08260 0.13 +0.3 1,652,934 + yhdE MCAODC_08260 0.13 +0.7 1,652,934 + yhdE MCAODC_08260 0.13 -0.1 1,652,934 + yhdE MCAODC_08260 0.13 -0.2 1,652,940 + yhdE MCAODC_08260 0.14 -0.2 1,652,986 + yhdE MCAODC_08260 0.22 -0.0 1,652,986 + yhdE MCAODC_08260 0.22 -0.1 1,652,986 + yhdE MCAODC_08260 0.22 +0.9 1,652,986 + yhdE MCAODC_08260 0.22 -0.9 1,652,987 - yhdE MCAODC_08260 0.22 -0.6 1,652,987 - yhdE MCAODC_08260 0.22 +0.2 1,653,070 - yhdE MCAODC_08260 0.36 +1.0 1,653,070 - yhdE MCAODC_08260 0.36 +0.7 1,653,144 - yhdE MCAODC_08260 0.48 -0.0 1,653,291 + yhdE MCAODC_08260 0.73 -0.9 1,653,292 - yhdE MCAODC_08260 0.73 -0.7 1,653,304 + yhdE MCAODC_08260 0.75 +2.9 1,653,305 - yhdE MCAODC_08260 0.75 +0.6 1,653,310 - yhdE MCAODC_08260 0.76 +0.2 1,653,345 - yhdE MCAODC_08260 0.82 +0.9 1,653,355 + yhdE MCAODC_08260 0.84 +0.7 1,653,355 + yhdE MCAODC_08260 0.84 +0.2 1,653,356 - yhdE MCAODC_08260 0.84 -0.2 1,653,356 - yhdE MCAODC_08260 0.84 +0.3
Or see this region's nucleotide sequence