Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fdnG and fdoH are separated by 12 nucleotides fdoH and fdoI overlap by 4 nucleotides fdoI and fdhE overlap by 4 nucleotides
MCAODC_04455: fdnG - formate dehydrogenase-N subunit alpha, at 901,695 to 904,745
fdnG
MCAODC_04460: fdoH - formate dehydrogenase O subunit beta, at 904,758 to 905,660
fdoH
MCAODC_04465: fdoI - formate dehydrogenase cytochrome b556 subunit, at 905,657 to 906,292
fdoI
MCAODC_04470: fdhE - formate dehydrogenase accessory protein FdhE, at 906,289 to 907,218
fdhE
Position (kb) 905
906
907 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 904.664 kb on + strand at 904.757 kb on + strand at 904.757 kb on + strand at 904.757 kb on + strand at 904.757 kb on + strand at 904.757 kb on + strand at 904.757 kb on + strand at 904.757 kb on + strand at 904.758 kb on - strand at 904.778 kb on + strand at 904.779 kb on - strand at 904.884 kb on - strand, within fdoH at 904.953 kb on + strand, within fdoH at 905.057 kb on + strand, within fdoH at 905.057 kb on + strand, within fdoH at 905.058 kb on - strand, within fdoH at 905.058 kb on - strand, within fdoH at 905.111 kb on + strand, within fdoH at 905.230 kb on - strand, within fdoH at 905.237 kb on + strand, within fdoH at 905.238 kb on - strand, within fdoH at 905.238 kb on - strand, within fdoH at 905.380 kb on - strand, within fdoH at 905.420 kb on + strand, within fdoH at 905.420 kb on + strand, within fdoH at 905.421 kb on - strand, within fdoH at 905.429 kb on + strand, within fdoH at 905.429 kb on + strand, within fdoH at 905.429 kb on + strand, within fdoH at 905.430 kb on - strand, within fdoH at 905.430 kb on - strand, within fdoH at 905.430 kb on - strand, within fdoH at 905.430 kb on - strand, within fdoH at 905.430 kb on - strand, within fdoH at 905.430 kb on - strand, within fdoH at 905.433 kb on + strand, within fdoH at 905.433 kb on + strand, within fdoH at 905.433 kb on + strand, within fdoH at 905.433 kb on + strand, within fdoH at 905.433 kb on + strand, within fdoH at 905.434 kb on - strand, within fdoH at 905.434 kb on - strand, within fdoH at 905.434 kb on - strand, within fdoH at 905.434 kb on - strand, within fdoH at 905.434 kb on - strand, within fdoH at 905.434 kb on - strand, within fdoH at 905.434 kb on - strand, within fdoH at 905.434 kb on - strand, within fdoH at 905.467 kb on - strand, within fdoH at 905.467 kb on - strand, within fdoH at 905.574 kb on - strand at 905.584 kb on - strand at 905.624 kb on + strand at 905.625 kb on - strand at 905.625 kb on - strand at 905.685 kb on - strand at 905.685 kb on - strand at 905.701 kb on + strand at 905.701 kb on + strand at 905.701 kb on + strand at 905.702 kb on - strand at 905.702 kb on - strand at 905.702 kb on - strand at 905.702 kb on - strand at 905.845 kb on + strand, within fdoI at 905.895 kb on + strand, within fdoI at 905.895 kb on + strand, within fdoI at 905.895 kb on + strand, within fdoI at 905.895 kb on + strand, within fdoI at 905.895 kb on + strand, within fdoI at 905.912 kb on - strand, within fdoI at 905.912 kb on - strand, within fdoI at 905.912 kb on - strand, within fdoI at 905.935 kb on + strand, within fdoI at 905.936 kb on - strand, within fdoI at 905.958 kb on + strand, within fdoI at 905.958 kb on + strand, within fdoI at 905.958 kb on + strand, within fdoI at 905.959 kb on - strand, within fdoI at 905.959 kb on - strand, within fdoI at 905.959 kb on - strand, within fdoI at 905.959 kb on - strand, within fdoI at 905.959 kb on - strand, within fdoI at 905.977 kb on + strand, within fdoI at 905.977 kb on + strand, within fdoI at 905.977 kb on + strand, within fdoI at 905.978 kb on - strand, within fdoI at 905.978 kb on - strand, within fdoI at 905.978 kb on - strand, within fdoI at 906.013 kb on + strand, within fdoI at 906.049 kb on + strand, within fdoI at 906.049 kb on + strand, within fdoI at 906.063 kb on - strand, within fdoI at 906.135 kb on + strand, within fdoI at 906.136 kb on - strand, within fdoI at 906.136 kb on - strand, within fdoI at 906.145 kb on + strand, within fdoI at 906.146 kb on - strand, within fdoI at 906.157 kb on + strand, within fdoI at 906.157 kb on + strand, within fdoI at 906.157 kb on + strand, within fdoI at 906.157 kb on + strand, within fdoI at 906.157 kb on + strand, within fdoI at 906.158 kb on - strand, within fdoI at 906.158 kb on - strand, within fdoI at 906.158 kb on - strand, within fdoI at 906.158 kb on - strand, within fdoI at 906.158 kb on - strand, within fdoI at 906.158 kb on - strand, within fdoI at 906.158 kb on - strand, within fdoI at 906.158 kb on - strand, within fdoI at 906.158 kb on - strand, within fdoI at 906.161 kb on + strand, within fdoI at 906.161 kb on + strand, within fdoI at 906.162 kb on - strand, within fdoI at 906.302 kb on + strand at 906.349 kb on - strand at 906.362 kb on + strand at 906.387 kb on - strand, within fdhE at 906.387 kb on - strand, within fdhE at 906.388 kb on + strand, within fdhE at 906.445 kb on + strand, within fdhE at 906.446 kb on - strand, within fdhE at 906.446 kb on - strand, within fdhE at 906.493 kb on + strand, within fdhE at 906.493 kb on + strand, within fdhE at 906.532 kb on - strand, within fdhE at 906.787 kb on + strand, within fdhE at 906.854 kb on - strand, within fdhE at 906.873 kb on + strand, within fdhE at 906.874 kb on - strand, within fdhE at 906.917 kb on + strand, within fdhE at 906.918 kb on - strand, within fdhE at 907.007 kb on - strand, within fdhE at 907.069 kb on + strand, within fdhE at 907.070 kb on - strand, within fdhE at 907.070 kb on - strand, within fdhE at 907.070 kb on - strand, within fdhE at 907.070 kb on - strand, within fdhE at 907.139 kb on + strand at 907.139 kb on + strand at 907.168 kb on + strand at 907.217 kb on - strand at 907.256 kb on + strand at 907.266 kb on - strand at 907.266 kb on - strand at 907.290 kb on + strand at 907.291 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 904,664 + -0.1 904,757 + +0.3 904,757 + +1.3 904,757 + -0.7 904,757 + -0.1 904,757 + +0.1 904,757 + +1.6 904,757 + -3.0 904,758 - -0.2 904,778 + -0.2 904,779 - +0.9 904,884 - fdoH MCAODC_04460 0.14 +1.1 904,953 + fdoH MCAODC_04460 0.22 +0.5 905,057 + fdoH MCAODC_04460 0.33 +1.3 905,057 + fdoH MCAODC_04460 0.33 +0.1 905,058 - fdoH MCAODC_04460 0.33 -1.0 905,058 - fdoH MCAODC_04460 0.33 +0.1 905,111 + fdoH MCAODC_04460 0.39 -0.6 905,230 - fdoH MCAODC_04460 0.52 +0.3 905,237 + fdoH MCAODC_04460 0.53 -0.4 905,238 - fdoH MCAODC_04460 0.53 +0.0 905,238 - fdoH MCAODC_04460 0.53 +1.0 905,380 - fdoH MCAODC_04460 0.69 +0.3 905,420 + fdoH MCAODC_04460 0.73 -0.7 905,420 + fdoH MCAODC_04460 0.73 -0.2 905,421 - fdoH MCAODC_04460 0.73 +0.8 905,429 + fdoH MCAODC_04460 0.74 +0.4 905,429 + fdoH MCAODC_04460 0.74 -0.7 905,429 + fdoH MCAODC_04460 0.74 +0.2 905,430 - fdoH MCAODC_04460 0.74 +0.0 905,430 - fdoH MCAODC_04460 0.74 -1.3 905,430 - fdoH MCAODC_04460 0.74 -0.0 905,430 - fdoH MCAODC_04460 0.74 -2.8 905,430 - fdoH MCAODC_04460 0.74 -1.9 905,430 - fdoH MCAODC_04460 0.74 -0.5 905,433 + fdoH MCAODC_04460 0.75 -0.2 905,433 + fdoH MCAODC_04460 0.75 -0.9 905,433 + fdoH MCAODC_04460 0.75 -2.5 905,433 + fdoH MCAODC_04460 0.75 -0.9 905,433 + fdoH MCAODC_04460 0.75 -0.2 905,434 - fdoH MCAODC_04460 0.75 +0.9 905,434 - fdoH MCAODC_04460 0.75 +0.3 905,434 - fdoH MCAODC_04460 0.75 +0.0 905,434 - fdoH MCAODC_04460 0.75 -0.3 905,434 - fdoH MCAODC_04460 0.75 -0.0 905,434 - fdoH MCAODC_04460 0.75 +0.1 905,434 - fdoH MCAODC_04460 0.75 -2.0 905,434 - fdoH MCAODC_04460 0.75 +1.8 905,467 - fdoH MCAODC_04460 0.79 -0.4 905,467 - fdoH MCAODC_04460 0.79 -1.2 905,574 - +0.4 905,584 - +0.5 905,624 + -0.3 905,625 - +1.3 905,625 - +2.1 905,685 - +0.4 905,685 - -0.7 905,701 + -0.3 905,701 + +0.2 905,701 + +0.7 905,702 - -0.8 905,702 - +0.1 905,702 - +0.2 905,702 - -0.5 905,845 + fdoI MCAODC_04465 0.30 +0.5 905,895 + fdoI MCAODC_04465 0.37 -1.7 905,895 + fdoI MCAODC_04465 0.37 -0.8 905,895 + fdoI MCAODC_04465 0.37 -2.0 905,895 + fdoI MCAODC_04465 0.37 -0.1 905,895 + fdoI MCAODC_04465 0.37 +1.0 905,912 - fdoI MCAODC_04465 0.40 +0.3 905,912 - fdoI MCAODC_04465 0.40 +2.0 905,912 - fdoI MCAODC_04465 0.40 +1.0 905,935 + fdoI MCAODC_04465 0.44 +0.1 905,936 - fdoI MCAODC_04465 0.44 +0.3 905,958 + fdoI MCAODC_04465 0.47 +1.5 905,958 + fdoI MCAODC_04465 0.47 -0.7 905,958 + fdoI MCAODC_04465 0.47 +0.7 905,959 - fdoI MCAODC_04465 0.47 +0.4 905,959 - fdoI MCAODC_04465 0.47 +0.4 905,959 - fdoI MCAODC_04465 0.47 +1.1 905,959 - fdoI MCAODC_04465 0.47 -1.0 905,959 - fdoI MCAODC_04465 0.47 -1.0 905,977 + fdoI MCAODC_04465 0.50 -0.4 905,977 + fdoI MCAODC_04465 0.50 -2.6 905,977 + fdoI MCAODC_04465 0.50 -0.8 905,978 - fdoI MCAODC_04465 0.50 +1.0 905,978 - fdoI MCAODC_04465 0.50 +1.2 905,978 - fdoI MCAODC_04465 0.50 -2.5 906,013 + fdoI MCAODC_04465 0.56 -0.9 906,049 + fdoI MCAODC_04465 0.62 +0.6 906,049 + fdoI MCAODC_04465 0.62 +1.2 906,063 - fdoI MCAODC_04465 0.64 +0.1 906,135 + fdoI MCAODC_04465 0.75 -0.5 906,136 - fdoI MCAODC_04465 0.75 +1.2 906,136 - fdoI MCAODC_04465 0.75 +1.4 906,145 + fdoI MCAODC_04465 0.77 +0.0 906,146 - fdoI MCAODC_04465 0.77 -0.1 906,157 + fdoI MCAODC_04465 0.79 -0.3 906,157 + fdoI MCAODC_04465 0.79 -0.0 906,157 + fdoI MCAODC_04465 0.79 -1.4 906,157 + fdoI MCAODC_04465 0.79 -0.7 906,157 + fdoI MCAODC_04465 0.79 +1.6 906,158 - fdoI MCAODC_04465 0.79 +1.1 906,158 - fdoI MCAODC_04465 0.79 -0.4 906,158 - fdoI MCAODC_04465 0.79 -0.3 906,158 - fdoI MCAODC_04465 0.79 -1.3 906,158 - fdoI MCAODC_04465 0.79 +1.7 906,158 - fdoI MCAODC_04465 0.79 -2.2 906,158 - fdoI MCAODC_04465 0.79 +1.3 906,158 - fdoI MCAODC_04465 0.79 +0.1 906,158 - fdoI MCAODC_04465 0.79 -1.0 906,161 + fdoI MCAODC_04465 0.79 -0.5 906,161 + fdoI MCAODC_04465 0.79 -0.1 906,162 - fdoI MCAODC_04465 0.79 -0.7 906,302 + +1.2 906,349 - +1.8 906,362 + -0.3 906,387 - fdhE MCAODC_04470 0.11 +0.2 906,387 - fdhE MCAODC_04470 0.11 -0.2 906,388 + fdhE MCAODC_04470 0.11 +0.4 906,445 + fdhE MCAODC_04470 0.17 -0.1 906,446 - fdhE MCAODC_04470 0.17 +0.2 906,446 - fdhE MCAODC_04470 0.17 +0.1 906,493 + fdhE MCAODC_04470 0.22 -1.0 906,493 + fdhE MCAODC_04470 0.22 +2.1 906,532 - fdhE MCAODC_04470 0.26 -0.3 906,787 + fdhE MCAODC_04470 0.54 -1.4 906,854 - fdhE MCAODC_04470 0.61 -0.5 906,873 + fdhE MCAODC_04470 0.63 +0.9 906,874 - fdhE MCAODC_04470 0.63 +1.4 906,917 + fdhE MCAODC_04470 0.68 -0.5 906,918 - fdhE MCAODC_04470 0.68 +0.2 907,007 - fdhE MCAODC_04470 0.77 +1.6 907,069 + fdhE MCAODC_04470 0.84 -0.0 907,070 - fdhE MCAODC_04470 0.84 +0.2 907,070 - fdhE MCAODC_04470 0.84 +0.7 907,070 - fdhE MCAODC_04470 0.84 +1.6 907,070 - fdhE MCAODC_04470 0.84 -1.1 907,139 + +0.2 907,139 + -0.0 907,168 + +1.5 907,217 - -0.8 907,256 + -0.1 907,266 - +0.5 907,266 - -0.9 907,290 + +3.5 907,291 - +1.4
Or see this region's nucleotide sequence