Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yiiQ and tpiA are separated by 107 nucleotides tpiA and cdh are separated by 54 nucleotides cdh and sbp are separated by 106 nucleotides
MCAODC_04305: yiiQ - Uncharacterized protein YiiQ, at 873,979 to 874,578
yiiQ
MCAODC_04310: tpiA - triose-phosphate isomerase, at 874,686 to 875,453
tpiA
MCAODC_04315: cdh - CDP-diacylglycerol diphosphatase, at 875,508 to 876,263
cdh
MCAODC_04320: sbp - sulfate/thiosulfate ABC transporter substrate-binding protein Sbp, at 876,370 to 877,359
sbp
Position (kb) 875
876
877 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 874.536 kb on + strand at 874.572 kb on + strand at 874.573 kb on - strand at 874.612 kb on - strand at 874.666 kb on + strand at 874.666 kb on + strand at 874.666 kb on + strand at 874.668 kb on + strand at 875.451 kb on + strand at 875.451 kb on + strand at 875.452 kb on - strand at 875.452 kb on - strand at 875.452 kb on - strand at 875.452 kb on - strand at 875.487 kb on + strand at 875.487 kb on + strand at 875.487 kb on + strand at 875.488 kb on - strand at 875.497 kb on - strand at 875.571 kb on + strand at 875.596 kb on - strand, within cdh at 875.596 kb on - strand, within cdh at 875.606 kb on + strand, within cdh at 875.607 kb on - strand, within cdh at 875.607 kb on - strand, within cdh at 875.620 kb on - strand, within cdh at 875.635 kb on + strand, within cdh at 875.645 kb on + strand, within cdh at 875.645 kb on + strand, within cdh at 875.646 kb on - strand, within cdh at 875.646 kb on - strand, within cdh at 875.664 kb on + strand, within cdh at 875.665 kb on - strand, within cdh at 875.684 kb on + strand, within cdh at 875.685 kb on - strand, within cdh at 875.685 kb on - strand, within cdh at 875.685 kb on - strand, within cdh at 875.685 kb on - strand, within cdh at 875.685 kb on - strand, within cdh at 875.701 kb on - strand, within cdh at 875.734 kb on + strand, within cdh at 875.734 kb on + strand, within cdh at 875.799 kb on - strand, within cdh at 875.828 kb on + strand, within cdh at 875.837 kb on + strand, within cdh at 875.837 kb on + strand, within cdh at 875.837 kb on + strand, within cdh at 875.837 kb on + strand, within cdh at 875.838 kb on - strand, within cdh at 875.861 kb on + strand, within cdh at 876.014 kb on + strand, within cdh at 876.015 kb on - strand, within cdh at 876.015 kb on - strand, within cdh at 876.020 kb on - strand, within cdh at 876.020 kb on - strand, within cdh at 876.020 kb on - strand, within cdh at 876.037 kb on + strand, within cdh at 876.037 kb on + strand, within cdh at 876.038 kb on - strand, within cdh at 876.038 kb on - strand, within cdh at 876.054 kb on - strand, within cdh at 876.073 kb on + strand, within cdh at 876.158 kb on + strand, within cdh at 876.158 kb on + strand, within cdh at 876.159 kb on - strand, within cdh at 876.159 kb on - strand, within cdh at 876.162 kb on + strand, within cdh at 876.162 kb on + strand, within cdh at 876.163 kb on - strand, within cdh at 876.163 kb on - strand, within cdh at 876.163 kb on - strand, within cdh at 876.163 kb on - strand, within cdh at 876.163 kb on - strand, within cdh at 876.167 kb on + strand, within cdh at 876.203 kb on + strand at 876.225 kb on - strand at 876.228 kb on + strand at 876.228 kb on + strand at 876.229 kb on - strand at 876.229 kb on - strand at 876.319 kb on - strand at 876.354 kb on - strand at 876.358 kb on - strand at 876.358 kb on - strand at 876.397 kb on - strand at 876.464 kb on - strand at 876.525 kb on - strand, within sbp at 876.527 kb on + strand, within sbp at 876.528 kb on - strand, within sbp at 876.528 kb on - strand, within sbp at 876.528 kb on - strand, within sbp at 876.564 kb on - strand, within sbp at 876.570 kb on - strand, within sbp at 876.597 kb on + strand, within sbp at 876.597 kb on + strand, within sbp at 876.655 kb on - strand, within sbp at 876.655 kb on - strand, within sbp at 876.684 kb on + strand, within sbp at 876.739 kb on + strand, within sbp at 876.771 kb on + strand, within sbp at 876.814 kb on - strand, within sbp at 876.816 kb on - strand, within sbp at 876.894 kb on - strand, within sbp at 876.894 kb on - strand, within sbp at 876.954 kb on + strand, within sbp at 876.955 kb on - strand, within sbp at 876.997 kb on - strand, within sbp at 877.000 kb on + strand, within sbp at 877.000 kb on + strand, within sbp at 877.001 kb on - strand, within sbp at 877.001 kb on - strand, within sbp at 877.025 kb on + strand, within sbp at 877.065 kb on + strand, within sbp at 877.103 kb on + strand, within sbp at 877.104 kb on - strand, within sbp at 877.201 kb on - strand, within sbp at 877.244 kb on + strand, within sbp at 877.244 kb on + strand, within sbp at 877.245 kb on - strand, within sbp at 877.245 kb on - strand, within sbp at 877.245 kb on - strand, within sbp at 877.245 kb on - strand, within sbp
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 874,536 + +0.1 874,572 + -0.1 874,573 - +0.5 874,612 - +0.1 874,666 + +0.3 874,666 + -0.6 874,666 + +0.7 874,668 + -0.9 875,451 + +0.2 875,451 + -1.0 875,452 - +2.7 875,452 - +0.3 875,452 - +0.9 875,452 - -0.2 875,487 + +0.9 875,487 + +1.1 875,487 + +0.4 875,488 - +0.4 875,497 - +0.1 875,571 + +0.6 875,596 - cdh MCAODC_04315 0.12 +0.4 875,596 - cdh MCAODC_04315 0.12 +0.3 875,606 + cdh MCAODC_04315 0.13 +0.1 875,607 - cdh MCAODC_04315 0.13 +0.7 875,607 - cdh MCAODC_04315 0.13 +0.5 875,620 - cdh MCAODC_04315 0.15 -1.3 875,635 + cdh MCAODC_04315 0.17 -0.5 875,645 + cdh MCAODC_04315 0.18 +0.6 875,645 + cdh MCAODC_04315 0.18 -0.1 875,646 - cdh MCAODC_04315 0.18 -0.6 875,646 - cdh MCAODC_04315 0.18 -1.8 875,664 + cdh MCAODC_04315 0.21 -0.5 875,665 - cdh MCAODC_04315 0.21 +0.5 875,684 + cdh MCAODC_04315 0.23 +0.6 875,685 - cdh MCAODC_04315 0.23 -0.4 875,685 - cdh MCAODC_04315 0.23 -0.4 875,685 - cdh MCAODC_04315 0.23 -0.1 875,685 - cdh MCAODC_04315 0.23 -0.1 875,685 - cdh MCAODC_04315 0.23 -0.2 875,701 - cdh MCAODC_04315 0.26 -1.0 875,734 + cdh MCAODC_04315 0.30 +1.5 875,734 + cdh MCAODC_04315 0.30 +0.6 875,799 - cdh MCAODC_04315 0.38 -0.5 875,828 + cdh MCAODC_04315 0.42 +0.2 875,837 + cdh MCAODC_04315 0.44 +0.3 875,837 + cdh MCAODC_04315 0.44 +0.3 875,837 + cdh MCAODC_04315 0.44 -1.4 875,837 + cdh MCAODC_04315 0.44 -0.4 875,838 - cdh MCAODC_04315 0.44 -2.5 875,861 + cdh MCAODC_04315 0.47 +1.0 876,014 + cdh MCAODC_04315 0.67 +0.8 876,015 - cdh MCAODC_04315 0.67 -0.0 876,015 - cdh MCAODC_04315 0.67 +0.4 876,020 - cdh MCAODC_04315 0.68 +0.8 876,020 - cdh MCAODC_04315 0.68 -1.3 876,020 - cdh MCAODC_04315 0.68 -0.2 876,037 + cdh MCAODC_04315 0.70 +0.3 876,037 + cdh MCAODC_04315 0.70 +0.1 876,038 - cdh MCAODC_04315 0.70 -1.0 876,038 - cdh MCAODC_04315 0.70 +0.5 876,054 - cdh MCAODC_04315 0.72 -0.2 876,073 + cdh MCAODC_04315 0.75 -1.2 876,158 + cdh MCAODC_04315 0.86 +1.2 876,158 + cdh MCAODC_04315 0.86 +1.2 876,159 - cdh MCAODC_04315 0.86 +0.7 876,159 - cdh MCAODC_04315 0.86 -0.4 876,162 + cdh MCAODC_04315 0.87 +0.7 876,162 + cdh MCAODC_04315 0.87 -2.1 876,163 - cdh MCAODC_04315 0.87 +0.5 876,163 - cdh MCAODC_04315 0.87 +1.4 876,163 - cdh MCAODC_04315 0.87 +0.0 876,163 - cdh MCAODC_04315 0.87 -0.4 876,163 - cdh MCAODC_04315 0.87 -1.4 876,167 + cdh MCAODC_04315 0.87 -1.6 876,203 + +0.4 876,225 - +0.8 876,228 + -0.5 876,228 + +0.0 876,229 - -0.2 876,229 - +0.2 876,319 - +0.1 876,354 - +0.4 876,358 - -0.4 876,358 - +0.2 876,397 - -0.0 876,464 - -3.3 876,525 - sbp MCAODC_04320 0.16 +0.5 876,527 + sbp MCAODC_04320 0.16 +0.1 876,528 - sbp MCAODC_04320 0.16 +1.1 876,528 - sbp MCAODC_04320 0.16 -0.0 876,528 - sbp MCAODC_04320 0.16 +0.3 876,564 - sbp MCAODC_04320 0.20 +0.0 876,570 - sbp MCAODC_04320 0.20 +0.3 876,597 + sbp MCAODC_04320 0.23 +0.8 876,597 + sbp MCAODC_04320 0.23 +0.3 876,655 - sbp MCAODC_04320 0.29 +0.4 876,655 - sbp MCAODC_04320 0.29 -0.8 876,684 + sbp MCAODC_04320 0.32 -1.3 876,739 + sbp MCAODC_04320 0.37 -1.5 876,771 + sbp MCAODC_04320 0.41 +1.3 876,814 - sbp MCAODC_04320 0.45 +0.8 876,816 - sbp MCAODC_04320 0.45 +1.6 876,894 - sbp MCAODC_04320 0.53 -0.2 876,894 - sbp MCAODC_04320 0.53 +0.0 876,954 + sbp MCAODC_04320 0.59 -1.6 876,955 - sbp MCAODC_04320 0.59 -0.5 876,997 - sbp MCAODC_04320 0.63 +0.3 877,000 + sbp MCAODC_04320 0.64 +1.4 877,000 + sbp MCAODC_04320 0.64 +1.3 877,001 - sbp MCAODC_04320 0.64 +1.9 877,001 - sbp MCAODC_04320 0.64 +1.2 877,025 + sbp MCAODC_04320 0.66 +0.2 877,065 + sbp MCAODC_04320 0.70 -3.2 877,103 + sbp MCAODC_04320 0.74 -2.7 877,104 - sbp MCAODC_04320 0.74 +0.4 877,201 - sbp MCAODC_04320 0.84 +0.6 877,244 + sbp MCAODC_04320 0.88 +0.6 877,244 + sbp MCAODC_04320 0.88 +0.3 877,245 - sbp MCAODC_04320 0.88 +0.8 877,245 - sbp MCAODC_04320 0.88 +1.9 877,245 - sbp MCAODC_04320 0.88 -0.3 877,245 - sbp MCAODC_04320 0.88 +1.6
Or see this region's nucleotide sequence