Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rbsA and MCAODC_03225 overlap by 4 nucleotides MCAODC_03225 and rbsB are separated by 18 nucleotides
MCAODC_03220: rbsA - Ribose import ATP-binding protein RbsA, at 647,726 to 649,246
rbsA
MCAODC_03225: MCAODC_03225 - ribose ABC transporter permease, at 649,243 to 650,217
_03225
MCAODC_03230: rbsB - transcriptional regulator, at 650,236 to 651,177
rbsB
Position (kb) 649
650
651 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 648.280 kb on + strand, within rbsA at 648.281 kb on - strand, within rbsA at 648.311 kb on - strand, within rbsA at 648.311 kb on - strand, within rbsA at 648.314 kb on + strand, within rbsA at 648.314 kb on + strand, within rbsA at 648.314 kb on + strand, within rbsA at 648.314 kb on + strand, within rbsA at 648.314 kb on + strand, within rbsA at 648.314 kb on + strand, within rbsA at 648.315 kb on - strand, within rbsA at 648.315 kb on - strand, within rbsA at 648.315 kb on - strand, within rbsA at 648.315 kb on - strand, within rbsA at 648.315 kb on - strand, within rbsA at 648.315 kb on - strand, within rbsA at 648.341 kb on + strand, within rbsA at 648.341 kb on + strand, within rbsA at 648.342 kb on - strand, within rbsA at 648.342 kb on - strand, within rbsA at 648.370 kb on - strand, within rbsA at 648.383 kb on + strand, within rbsA at 648.384 kb on - strand, within rbsA at 648.384 kb on - strand, within rbsA at 648.469 kb on + strand, within rbsA at 648.756 kb on + strand, within rbsA at 648.757 kb on - strand, within rbsA at 648.868 kb on + strand, within rbsA at 648.868 kb on + strand, within rbsA at 648.869 kb on - strand, within rbsA at 648.869 kb on - strand, within rbsA at 648.869 kb on - strand, within rbsA at 648.904 kb on - strand, within rbsA at 648.904 kb on - strand, within rbsA at 648.904 kb on - strand, within rbsA at 649.104 kb on + strand at 649.127 kb on + strand at 649.127 kb on + strand at 649.128 kb on - strand at 649.128 kb on - strand at 649.161 kb on + strand at 649.162 kb on - strand at 649.192 kb on + strand at 649.192 kb on + strand at 649.192 kb on + strand at 649.193 kb on - strand at 649.193 kb on - strand at 649.193 kb on - strand at 649.193 kb on - strand at 649.200 kb on + strand at 649.201 kb on - strand at 649.389 kb on + strand, within MCAODC_03225 at 649.522 kb on - strand, within MCAODC_03225 at 649.645 kb on + strand, within MCAODC_03225 at 649.645 kb on + strand, within MCAODC_03225 at 649.646 kb on - strand, within MCAODC_03225 at 649.646 kb on - strand, within MCAODC_03225 at 649.646 kb on - strand, within MCAODC_03225 at 649.648 kb on - strand, within MCAODC_03225 at 649.648 kb on - strand, within MCAODC_03225 at 649.695 kb on + strand, within MCAODC_03225 at 649.710 kb on + strand, within MCAODC_03225 at 649.735 kb on + strand, within MCAODC_03225 at 649.735 kb on + strand, within MCAODC_03225 at 649.735 kb on + strand, within MCAODC_03225 at 649.736 kb on - strand, within MCAODC_03225 at 649.736 kb on - strand, within MCAODC_03225 at 649.736 kb on - strand, within MCAODC_03225 at 649.736 kb on - strand, within MCAODC_03225 at 649.736 kb on - strand, within MCAODC_03225 at 649.736 kb on - strand, within MCAODC_03225 at 649.736 kb on - strand, within MCAODC_03225 at 649.827 kb on + strand, within MCAODC_03225 at 649.827 kb on + strand, within MCAODC_03225 at 649.828 kb on - strand, within MCAODC_03225 at 649.828 kb on - strand, within MCAODC_03225 at 649.828 kb on - strand, within MCAODC_03225 at 649.831 kb on + strand, within MCAODC_03225 at 649.831 kb on + strand, within MCAODC_03225 at 649.867 kb on - strand, within MCAODC_03225 at 649.867 kb on - strand, within MCAODC_03225 at 649.875 kb on + strand, within MCAODC_03225 at 649.875 kb on + strand, within MCAODC_03225 at 649.876 kb on - strand, within MCAODC_03225 at 649.904 kb on - strand, within MCAODC_03225 at 649.960 kb on + strand, within MCAODC_03225 at 649.979 kb on + strand, within MCAODC_03225 at 649.979 kb on + strand, within MCAODC_03225 at 649.980 kb on - strand, within MCAODC_03225 at 649.980 kb on - strand, within MCAODC_03225 at 649.980 kb on - strand, within MCAODC_03225 at 649.980 kb on - strand, within MCAODC_03225 at 650.081 kb on + strand, within MCAODC_03225 at 650.085 kb on + strand, within MCAODC_03225 at 650.128 kb on + strand at 650.128 kb on + strand at 650.128 kb on + strand at 650.129 kb on - strand at 650.129 kb on - strand at 650.189 kb on - strand at 650.230 kb on + strand at 650.231 kb on - strand at 650.231 kb on - strand at 650.331 kb on - strand, within rbsB at 650.444 kb on - strand, within rbsB at 650.471 kb on + strand, within rbsB at 650.472 kb on - strand, within rbsB at 650.472 kb on - strand, within rbsB at 650.505 kb on + strand, within rbsB at 650.592 kb on - strand, within rbsB at 650.655 kb on + strand, within rbsB at 650.655 kb on + strand, within rbsB at 650.656 kb on - strand, within rbsB at 650.656 kb on - strand, within rbsB at 650.659 kb on + strand, within rbsB at 650.659 kb on + strand, within rbsB at 650.659 kb on + strand, within rbsB at 650.659 kb on + strand, within rbsB at 650.659 kb on + strand, within rbsB at 650.659 kb on + strand, within rbsB at 650.659 kb on + strand, within rbsB at 650.660 kb on - strand, within rbsB at 650.660 kb on - strand, within rbsB at 650.660 kb on - strand, within rbsB at 650.660 kb on - strand, within rbsB at 650.660 kb on - strand, within rbsB at 650.660 kb on - strand, within rbsB at 650.660 kb on - strand, within rbsB at 650.756 kb on - strand, within rbsB at 650.834 kb on + strand, within rbsB at 650.834 kb on + strand, within rbsB at 650.834 kb on + strand, within rbsB at 650.835 kb on - strand, within rbsB at 650.876 kb on + strand, within rbsB at 650.876 kb on + strand, within rbsB at 650.938 kb on - strand, within rbsB at 650.949 kb on + strand, within rbsB at 651.154 kb on + strand at 651.155 kb on - strand at 651.155 kb on - strand at 651.175 kb on + strand at 651.175 kb on + strand at 651.176 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 648,280 + rbsA MCAODC_03220 0.36 +1.0 648,281 - rbsA MCAODC_03220 0.36 +0.1 648,311 - rbsA MCAODC_03220 0.38 -2.7 648,311 - rbsA MCAODC_03220 0.38 +0.3 648,314 + rbsA MCAODC_03220 0.39 -2.5 648,314 + rbsA MCAODC_03220 0.39 -1.2 648,314 + rbsA MCAODC_03220 0.39 +1.4 648,314 + rbsA MCAODC_03220 0.39 +0.8 648,314 + rbsA MCAODC_03220 0.39 +1.0 648,314 + rbsA MCAODC_03220 0.39 +0.6 648,315 - rbsA MCAODC_03220 0.39 -1.4 648,315 - rbsA MCAODC_03220 0.39 -3.1 648,315 - rbsA MCAODC_03220 0.39 -0.1 648,315 - rbsA MCAODC_03220 0.39 -0.1 648,315 - rbsA MCAODC_03220 0.39 -2.4 648,315 - rbsA MCAODC_03220 0.39 +0.2 648,341 + rbsA MCAODC_03220 0.40 +0.4 648,341 + rbsA MCAODC_03220 0.40 +2.1 648,342 - rbsA MCAODC_03220 0.40 +0.1 648,342 - rbsA MCAODC_03220 0.40 +0.1 648,370 - rbsA MCAODC_03220 0.42 +2.1 648,383 + rbsA MCAODC_03220 0.43 -0.6 648,384 - rbsA MCAODC_03220 0.43 -0.0 648,384 - rbsA MCAODC_03220 0.43 +0.4 648,469 + rbsA MCAODC_03220 0.49 -0.7 648,756 + rbsA MCAODC_03220 0.68 -2.3 648,757 - rbsA MCAODC_03220 0.68 -1.8 648,868 + rbsA MCAODC_03220 0.75 +0.5 648,868 + rbsA MCAODC_03220 0.75 +0.4 648,869 - rbsA MCAODC_03220 0.75 +0.8 648,869 - rbsA MCAODC_03220 0.75 +1.9 648,869 - rbsA MCAODC_03220 0.75 +0.3 648,904 - rbsA MCAODC_03220 0.77 -0.3 648,904 - rbsA MCAODC_03220 0.77 +0.9 648,904 - rbsA MCAODC_03220 0.77 +0.4 649,104 + -0.7 649,127 + +1.1 649,127 + -1.1 649,128 - +1.7 649,128 - +0.0 649,161 + -0.2 649,162 - +2.7 649,192 + +1.1 649,192 + -1.0 649,192 + +0.7 649,193 - -2.5 649,193 - -0.3 649,193 - -0.4 649,193 - -0.5 649,200 + +0.2 649,201 - +0.2 649,389 + MCAODC_03225 0.15 +0.3 649,522 - MCAODC_03225 0.29 +0.9 649,645 + MCAODC_03225 0.41 -1.6 649,645 + MCAODC_03225 0.41 -0.0 649,646 - MCAODC_03225 0.41 -0.0 649,646 - MCAODC_03225 0.41 +0.5 649,646 - MCAODC_03225 0.41 +1.3 649,648 - MCAODC_03225 0.42 -1.7 649,648 - MCAODC_03225 0.42 -0.2 649,695 + MCAODC_03225 0.46 +1.1 649,710 + MCAODC_03225 0.48 -1.0 649,735 + MCAODC_03225 0.50 +0.7 649,735 + MCAODC_03225 0.50 +2.1 649,735 + MCAODC_03225 0.50 +0.3 649,736 - MCAODC_03225 0.51 -0.4 649,736 - MCAODC_03225 0.51 +0.6 649,736 - MCAODC_03225 0.51 +1.2 649,736 - MCAODC_03225 0.51 +1.2 649,736 - MCAODC_03225 0.51 +0.9 649,736 - MCAODC_03225 0.51 +1.7 649,736 - MCAODC_03225 0.51 +0.7 649,827 + MCAODC_03225 0.60 +1.0 649,827 + MCAODC_03225 0.60 +0.1 649,828 - MCAODC_03225 0.60 -0.1 649,828 - MCAODC_03225 0.60 +1.1 649,828 - MCAODC_03225 0.60 +2.2 649,831 + MCAODC_03225 0.60 -0.0 649,831 + MCAODC_03225 0.60 +0.9 649,867 - MCAODC_03225 0.64 +0.1 649,867 - MCAODC_03225 0.64 +0.8 649,875 + MCAODC_03225 0.65 +1.1 649,875 + MCAODC_03225 0.65 +0.9 649,876 - MCAODC_03225 0.65 -0.5 649,904 - MCAODC_03225 0.68 -0.1 649,960 + MCAODC_03225 0.74 -0.5 649,979 + MCAODC_03225 0.75 -1.1 649,979 + MCAODC_03225 0.75 -0.5 649,980 - MCAODC_03225 0.76 -0.1 649,980 - MCAODC_03225 0.76 +0.2 649,980 - MCAODC_03225 0.76 +0.5 649,980 - MCAODC_03225 0.76 -0.3 650,081 + MCAODC_03225 0.86 -0.0 650,085 + MCAODC_03225 0.86 -0.1 650,128 + -1.5 650,128 + +0.0 650,128 + +0.8 650,129 - +0.5 650,129 - +0.3 650,189 - +0.1 650,230 + +1.6 650,231 - +0.3 650,231 - -0.5 650,331 - rbsB MCAODC_03230 0.10 +0.0 650,444 - rbsB MCAODC_03230 0.22 -0.4 650,471 + rbsB MCAODC_03230 0.25 +0.7 650,472 - rbsB MCAODC_03230 0.25 -2.8 650,472 - rbsB MCAODC_03230 0.25 +1.4 650,505 + rbsB MCAODC_03230 0.29 -0.2 650,592 - rbsB MCAODC_03230 0.38 +0.1 650,655 + rbsB MCAODC_03230 0.44 -0.7 650,655 + rbsB MCAODC_03230 0.44 +0.8 650,656 - rbsB MCAODC_03230 0.45 +0.1 650,656 - rbsB MCAODC_03230 0.45 +0.4 650,659 + rbsB MCAODC_03230 0.45 +0.2 650,659 + rbsB MCAODC_03230 0.45 +0.1 650,659 + rbsB MCAODC_03230 0.45 +0.3 650,659 + rbsB MCAODC_03230 0.45 -0.8 650,659 + rbsB MCAODC_03230 0.45 +0.6 650,659 + rbsB MCAODC_03230 0.45 -0.4 650,659 + rbsB MCAODC_03230 0.45 +1.7 650,660 - rbsB MCAODC_03230 0.45 +0.2 650,660 - rbsB MCAODC_03230 0.45 -0.4 650,660 - rbsB MCAODC_03230 0.45 +0.5 650,660 - rbsB MCAODC_03230 0.45 +0.7 650,660 - rbsB MCAODC_03230 0.45 -0.2 650,660 - rbsB MCAODC_03230 0.45 -0.5 650,660 - rbsB MCAODC_03230 0.45 +0.0 650,756 - rbsB MCAODC_03230 0.55 +0.5 650,834 + rbsB MCAODC_03230 0.63 +0.7 650,834 + rbsB MCAODC_03230 0.63 +2.1 650,834 + rbsB MCAODC_03230 0.63 -1.4 650,835 - rbsB MCAODC_03230 0.64 +0.0 650,876 + rbsB MCAODC_03230 0.68 -0.6 650,876 + rbsB MCAODC_03230 0.68 -0.1 650,938 - rbsB MCAODC_03230 0.75 +0.7 650,949 + rbsB MCAODC_03230 0.76 -1.0 651,154 + +0.4 651,155 - +0.8 651,155 - +0.4 651,175 + +0.3 651,175 + +1.0 651,176 - -0.3
Or see this region's nucleotide sequence