Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt phnJ and phnK overlap by 4 nucleotides phnK and phnL are separated by 110 nucleotides phnL and phnM overlap by 4 nucleotides
MCAODC_03175: phnJ - alpha-D-ribose 1-methylphosphonate 5-phosphate C-P-lyase PhnJ, at 639,604 to 640,449
phnJ
MCAODC_03180: phnK - phosphonate C-P lyase system protein PhnK, at 640,446 to 641,204
phnK
MCAODC_03185: phnL - phosphonate C-P lyase system protein PhnL, at 641,315 to 641,995
phnL
MCAODC_03190: phnM - alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase, at 641,992 to 643,128
phnM
Position (kb) 641
642 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 640.351 kb on + strand, within phnJ at 640.351 kb on + strand, within phnJ at 640.352 kb on - strand, within phnJ at 640.352 kb on - strand, within phnJ at 640.459 kb on + strand at 640.459 kb on + strand at 640.489 kb on - strand at 640.508 kb on + strand at 640.508 kb on + strand at 640.509 kb on - strand at 640.528 kb on + strand, within phnK at 640.529 kb on - strand, within phnK at 640.653 kb on + strand, within phnK at 640.705 kb on + strand, within phnK at 640.949 kb on + strand, within phnK at 641.042 kb on - strand, within phnK at 641.168 kb on + strand at 641.168 kb on + strand at 641.168 kb on + strand at 641.169 kb on - strand at 641.169 kb on - strand at 641.225 kb on - strand at 641.260 kb on - strand at 641.260 kb on - strand at 641.269 kb on - strand at 641.269 kb on - strand at 641.320 kb on + strand at 641.326 kb on - strand at 641.326 kb on - strand at 641.326 kb on - strand at 641.326 kb on - strand at 641.339 kb on - strand at 641.339 kb on - strand at 641.339 kb on - strand at 641.486 kb on + strand, within phnL at 641.486 kb on + strand, within phnL at 641.486 kb on + strand, within phnL at 641.486 kb on + strand, within phnL at 641.487 kb on - strand, within phnL at 641.487 kb on - strand, within phnL at 641.487 kb on - strand, within phnL at 641.536 kb on - strand, within phnL at 641.643 kb on + strand, within phnL at 641.644 kb on - strand, within phnL at 641.644 kb on - strand, within phnL at 641.646 kb on + strand, within phnL at 641.646 kb on + strand, within phnL at 641.647 kb on - strand, within phnL at 641.647 kb on - strand, within phnL at 641.647 kb on - strand, within phnL at 641.647 kb on - strand, within phnL at 641.806 kb on + strand, within phnL at 641.843 kb on - strand, within phnL at 641.946 kb on + strand at 641.950 kb on + strand at 641.950 kb on + strand at 641.950 kb on + strand at 641.950 kb on + strand at 641.950 kb on + strand at 641.950 kb on + strand at 641.950 kb on + strand at 641.950 kb on + strand at 641.951 kb on - strand at 641.951 kb on - strand at 641.951 kb on - strand at 641.951 kb on - strand at 641.951 kb on - strand at 641.951 kb on - strand at 641.951 kb on - strand at 642.003 kb on + strand at 642.036 kb on - strand at 642.156 kb on + strand, within phnM at 642.156 kb on + strand, within phnM at 642.156 kb on + strand, within phnM at 642.156 kb on + strand, within phnM at 642.157 kb on - strand, within phnM at 642.157 kb on - strand, within phnM at 642.157 kb on - strand, within phnM at 642.157 kb on - strand, within phnM at 642.172 kb on - strand, within phnM at 642.415 kb on - strand, within phnM at 642.524 kb on - strand, within phnM at 642.533 kb on - strand, within phnM at 642.547 kb on + strand, within phnM at 642.577 kb on + strand, within phnM at 642.577 kb on + strand, within phnM at 642.577 kb on + strand, within phnM at 642.578 kb on - strand, within phnM at 642.578 kb on - strand, within phnM at 642.578 kb on - strand, within phnM at 642.578 kb on - strand, within phnM at 642.810 kb on + strand, within phnM at 642.811 kb on - strand, within phnM at 642.895 kb on + strand, within phnM at 642.896 kb on - strand, within phnM at 642.896 kb on - strand, within phnM at 642.896 kb on - strand, within phnM
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 640,351 + phnJ MCAODC_03175 0.88 -0.1 640,351 + phnJ MCAODC_03175 0.88 -0.9 640,352 - phnJ MCAODC_03175 0.88 +0.2 640,352 - phnJ MCAODC_03175 0.88 +0.2 640,459 + -2.3 640,459 + +1.6 640,489 - -0.1 640,508 + -0.0 640,508 + -0.1 640,509 - -0.8 640,528 + phnK MCAODC_03180 0.11 +0.2 640,529 - phnK MCAODC_03180 0.11 +1.2 640,653 + phnK MCAODC_03180 0.27 -1.9 640,705 + phnK MCAODC_03180 0.34 +0.7 640,949 + phnK MCAODC_03180 0.66 +0.9 641,042 - phnK MCAODC_03180 0.79 -1.0 641,168 + -0.5 641,168 + +0.4 641,168 + -0.9 641,169 - +0.5 641,169 - -0.1 641,225 - -1.9 641,260 - -0.0 641,260 - +3.6 641,269 - +0.2 641,269 - +2.1 641,320 + +0.7 641,326 - -0.7 641,326 - -0.4 641,326 - +1.1 641,326 - -0.2 641,339 - +1.2 641,339 - +0.7 641,339 - -2.4 641,486 + phnL MCAODC_03185 0.25 +0.4 641,486 + phnL MCAODC_03185 0.25 -0.7 641,486 + phnL MCAODC_03185 0.25 +0.4 641,486 + phnL MCAODC_03185 0.25 -3.2 641,487 - phnL MCAODC_03185 0.25 +0.3 641,487 - phnL MCAODC_03185 0.25 -0.3 641,487 - phnL MCAODC_03185 0.25 +0.1 641,536 - phnL MCAODC_03185 0.32 -0.8 641,643 + phnL MCAODC_03185 0.48 -0.1 641,644 - phnL MCAODC_03185 0.48 -1.0 641,644 - phnL MCAODC_03185 0.48 -1.7 641,646 + phnL MCAODC_03185 0.49 +0.9 641,646 + phnL MCAODC_03185 0.49 +0.2 641,647 - phnL MCAODC_03185 0.49 +1.1 641,647 - phnL MCAODC_03185 0.49 +0.3 641,647 - phnL MCAODC_03185 0.49 +0.5 641,647 - phnL MCAODC_03185 0.49 +1.5 641,806 + phnL MCAODC_03185 0.72 -0.3 641,843 - phnL MCAODC_03185 0.78 +0.4 641,946 + +0.7 641,950 + +0.6 641,950 + +1.1 641,950 + +1.5 641,950 + +2.1 641,950 + +1.5 641,950 + +0.6 641,950 + +0.4 641,950 + -2.1 641,951 - +0.6 641,951 - +0.3 641,951 - +0.9 641,951 - +0.8 641,951 - +1.6 641,951 - -1.0 641,951 - +0.5 642,003 + +2.0 642,036 - +1.3 642,156 + phnM MCAODC_03190 0.14 -0.0 642,156 + phnM MCAODC_03190 0.14 +0.7 642,156 + phnM MCAODC_03190 0.14 -0.3 642,156 + phnM MCAODC_03190 0.14 -0.7 642,157 - phnM MCAODC_03190 0.15 +0.4 642,157 - phnM MCAODC_03190 0.15 +2.1 642,157 - phnM MCAODC_03190 0.15 +0.3 642,157 - phnM MCAODC_03190 0.15 -0.3 642,172 - phnM MCAODC_03190 0.16 +0.8 642,415 - phnM MCAODC_03190 0.37 +2.5 642,524 - phnM MCAODC_03190 0.47 -0.6 642,533 - phnM MCAODC_03190 0.48 +0.7 642,547 + phnM MCAODC_03190 0.49 +1.0 642,577 + phnM MCAODC_03190 0.51 +0.9 642,577 + phnM MCAODC_03190 0.51 +1.7 642,577 + phnM MCAODC_03190 0.51 +0.3 642,578 - phnM MCAODC_03190 0.52 -0.4 642,578 - phnM MCAODC_03190 0.52 +0.3 642,578 - phnM MCAODC_03190 0.52 -0.1 642,578 - phnM MCAODC_03190 0.52 +1.6 642,810 + phnM MCAODC_03190 0.72 +0.3 642,811 - phnM MCAODC_03190 0.72 +0.8 642,895 + phnM MCAODC_03190 0.79 +0.0 642,896 - phnM MCAODC_03190 0.80 +1.1 642,896 - phnM MCAODC_03190 0.80 -1.3 642,896 - phnM MCAODC_03190 0.80 -0.5
Or see this region's nucleotide sequence