Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ulaD and ulaC are separated by 13 nucleotides ulaC and ulaB are separated by 9 nucleotides ulaB and ulaA are separated by 15 nucleotides
MCAODC_02650: ulaD - 3-keto-L-gulonate-6-phosphate decarboxylase UlaD, at 537,502 to 538,152
ulaD
MCAODC_02655: ulaC - PTS ascorbate transporter subunit IIA, at 538,166 to 538,630
ulaC
MCAODC_02660: ulaB - PTS ascorbate transporter subunit IIB, at 538,640 to 538,945
ulaB
MCAODC_02665: ulaA - PTS ascorbate transporter subunit IIC, at 538,961 to 540,358
ulaA
Position (kb) 538
539 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 537.749 kb on + strand, within ulaD at 537.895 kb on + strand, within ulaD at 537.895 kb on + strand, within ulaD at 537.930 kb on + strand, within ulaD at 537.930 kb on + strand, within ulaD at 537.930 kb on + strand, within ulaD at 537.931 kb on - strand, within ulaD at 537.992 kb on + strand, within ulaD at 537.993 kb on - strand, within ulaD at 538.110 kb on + strand at 538.111 kb on - strand at 538.111 kb on - strand at 538.111 kb on - strand at 538.145 kb on - strand at 538.167 kb on + strand at 538.167 kb on + strand at 538.168 kb on - strand at 538.203 kb on - strand at 538.203 kb on - strand at 538.298 kb on - strand, within ulaC at 538.298 kb on - strand, within ulaC at 538.382 kb on + strand, within ulaC at 538.406 kb on - strand, within ulaC at 538.461 kb on + strand, within ulaC at 538.462 kb on - strand, within ulaC at 538.462 kb on - strand, within ulaC at 538.501 kb on - strand, within ulaC at 538.501 kb on - strand, within ulaC at 538.600 kb on + strand at 538.622 kb on + strand at 538.622 kb on + strand at 538.622 kb on + strand at 538.734 kb on + strand, within ulaB at 538.734 kb on + strand, within ulaB at 538.734 kb on + strand, within ulaB at 538.734 kb on + strand, within ulaB at 538.735 kb on - strand, within ulaB at 538.735 kb on - strand, within ulaB at 538.775 kb on - strand, within ulaB at 538.775 kb on - strand, within ulaB at 538.815 kb on + strand, within ulaB at 538.839 kb on - strand, within ulaB at 538.933 kb on + strand at 538.933 kb on + strand at 538.934 kb on - strand at 538.934 kb on - strand at 538.938 kb on - strand at 538.938 kb on - strand at 538.960 kb on - strand at 538.960 kb on - strand at 538.962 kb on + strand at 538.963 kb on - strand at 539.032 kb on + strand at 539.032 kb on + strand at 539.032 kb on + strand at 539.032 kb on + strand at 539.032 kb on + strand at 539.032 kb on + strand at 539.032 kb on + strand at 539.032 kb on + strand at 539.032 kb on + strand at 539.033 kb on - strand at 539.033 kb on - strand at 539.033 kb on - strand at 539.033 kb on - strand at 539.033 kb on - strand at 539.033 kb on - strand at 539.033 kb on - strand at 539.033 kb on - strand at 539.033 kb on - strand at 539.033 kb on - strand at 539.033 kb on - strand at 539.033 kb on - strand at 539.033 kb on - strand at 539.033 kb on - strand at 539.033 kb on - strand at 539.033 kb on - strand at 539.033 kb on - strand at 539.034 kb on + strand at 539.034 kb on + strand at 539.034 kb on + strand at 539.034 kb on + strand at 539.034 kb on + strand at 539.034 kb on + strand at 539.034 kb on + strand at 539.035 kb on - strand at 539.035 kb on - strand at 539.035 kb on - strand at 539.035 kb on - strand at 539.035 kb on - strand at 539.035 kb on - strand at 539.048 kb on + strand at 539.049 kb on - strand at 539.149 kb on + strand, within ulaA at 539.295 kb on - strand, within ulaA at 539.300 kb on - strand, within ulaA at 539.368 kb on + strand, within ulaA at 539.369 kb on - strand, within ulaA at 539.401 kb on + strand, within ulaA at 539.402 kb on - strand, within ulaA at 539.402 kb on - strand, within ulaA at 539.403 kb on + strand, within ulaA at 539.403 kb on + strand, within ulaA at 539.403 kb on + strand, within ulaA at 539.403 kb on + strand, within ulaA at 539.404 kb on - strand, within ulaA at 539.404 kb on - strand, within ulaA at 539.404 kb on - strand, within ulaA at 539.404 kb on - strand, within ulaA at 539.408 kb on - strand, within ulaA at 539.408 kb on - strand, within ulaA at 539.458 kb on + strand, within ulaA at 539.464 kb on + strand, within ulaA at 539.464 kb on + strand, within ulaA at 539.464 kb on + strand, within ulaA at 539.465 kb on - strand, within ulaA at 539.556 kb on + strand, within ulaA at 539.556 kb on + strand, within ulaA at 539.557 kb on - strand, within ulaA at 539.557 kb on - strand, within ulaA at 539.748 kb on + strand, within ulaA at 539.749 kb on - strand, within ulaA at 539.826 kb on + strand, within ulaA at 539.826 kb on + strand, within ulaA at 539.826 kb on + strand, within ulaA at 539.827 kb on - strand, within ulaA at 539.827 kb on - strand, within ulaA at 539.853 kb on + strand, within ulaA at 539.881 kb on + strand, within ulaA at 539.881 kb on + strand, within ulaA at 539.881 kb on + strand, within ulaA at 539.881 kb on + strand, within ulaA at 539.882 kb on - strand, within ulaA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 537,749 + ulaD MCAODC_02650 0.38 -0.1 537,895 + ulaD MCAODC_02650 0.60 -0.3 537,895 + ulaD MCAODC_02650 0.60 +0.4 537,930 + ulaD MCAODC_02650 0.66 -0.3 537,930 + ulaD MCAODC_02650 0.66 +1.1 537,930 + ulaD MCAODC_02650 0.66 -0.4 537,931 - ulaD MCAODC_02650 0.66 +1.0 537,992 + ulaD MCAODC_02650 0.75 -0.9 537,993 - ulaD MCAODC_02650 0.75 -0.6 538,110 + -0.1 538,111 - +1.6 538,111 - -0.1 538,111 - +1.6 538,145 - +0.7 538,167 + +0.2 538,167 + +0.0 538,168 - +0.4 538,203 - +0.8 538,203 - -0.0 538,298 - ulaC MCAODC_02655 0.28 -0.3 538,298 - ulaC MCAODC_02655 0.28 -0.1 538,382 + ulaC MCAODC_02655 0.46 -0.4 538,406 - ulaC MCAODC_02655 0.52 -0.9 538,461 + ulaC MCAODC_02655 0.63 -0.4 538,462 - ulaC MCAODC_02655 0.64 -0.4 538,462 - ulaC MCAODC_02655 0.64 -2.1 538,501 - ulaC MCAODC_02655 0.72 +0.6 538,501 - ulaC MCAODC_02655 0.72 -0.4 538,600 + +0.7 538,622 + +0.5 538,622 + +0.7 538,622 + +0.2 538,734 + ulaB MCAODC_02660 0.31 -0.2 538,734 + ulaB MCAODC_02660 0.31 +0.0 538,734 + ulaB MCAODC_02660 0.31 +1.3 538,734 + ulaB MCAODC_02660 0.31 +0.3 538,735 - ulaB MCAODC_02660 0.31 -0.1 538,735 - ulaB MCAODC_02660 0.31 +0.1 538,775 - ulaB MCAODC_02660 0.44 -0.2 538,775 - ulaB MCAODC_02660 0.44 -0.2 538,815 + ulaB MCAODC_02660 0.57 +0.9 538,839 - ulaB MCAODC_02660 0.65 -0.9 538,933 + -0.6 538,933 + +1.4 538,934 - -0.1 538,934 - +0.4 538,938 - -0.4 538,938 - +0.1 538,960 - -0.3 538,960 - +0.6 538,962 + +0.5 538,963 - +0.3 539,032 + +1.2 539,032 + -0.1 539,032 + +1.7 539,032 + -1.5 539,032 + -0.4 539,032 + +0.8 539,032 + +0.3 539,032 + +0.4 539,032 + -0.8 539,033 - +0.9 539,033 - -0.1 539,033 - +1.2 539,033 - -1.4 539,033 - -0.1 539,033 - -2.2 539,033 - -0.7 539,033 - -0.8 539,033 - -0.0 539,033 - +0.4 539,033 - -1.0 539,033 - +1.5 539,033 - -0.6 539,033 - -0.2 539,033 - -0.3 539,033 - -0.8 539,033 - +1.8 539,034 + -0.8 539,034 + +0.6 539,034 + +1.1 539,034 + -0.2 539,034 + -0.9 539,034 + -0.9 539,034 + +1.5 539,035 - +0.6 539,035 - -2.5 539,035 - +0.3 539,035 - +1.0 539,035 - -1.9 539,035 - +1.1 539,048 + +0.2 539,049 - +0.8 539,149 + ulaA MCAODC_02665 0.13 +1.7 539,295 - ulaA MCAODC_02665 0.24 +1.0 539,300 - ulaA MCAODC_02665 0.24 +1.0 539,368 + ulaA MCAODC_02665 0.29 -0.2 539,369 - ulaA MCAODC_02665 0.29 -1.0 539,401 + ulaA MCAODC_02665 0.31 +0.1 539,402 - ulaA MCAODC_02665 0.32 +0.7 539,402 - ulaA MCAODC_02665 0.32 +1.3 539,403 + ulaA MCAODC_02665 0.32 +0.6 539,403 + ulaA MCAODC_02665 0.32 +1.8 539,403 + ulaA MCAODC_02665 0.32 +0.0 539,403 + ulaA MCAODC_02665 0.32 -2.4 539,404 - ulaA MCAODC_02665 0.32 -0.1 539,404 - ulaA MCAODC_02665 0.32 +0.8 539,404 - ulaA MCAODC_02665 0.32 +0.6 539,404 - ulaA MCAODC_02665 0.32 -0.3 539,408 - ulaA MCAODC_02665 0.32 -0.1 539,408 - ulaA MCAODC_02665 0.32 +0.1 539,458 + ulaA MCAODC_02665 0.36 -1.8 539,464 + ulaA MCAODC_02665 0.36 -0.9 539,464 + ulaA MCAODC_02665 0.36 +1.5 539,464 + ulaA MCAODC_02665 0.36 +0.7 539,465 - ulaA MCAODC_02665 0.36 -2.4 539,556 + ulaA MCAODC_02665 0.43 +0.7 539,556 + ulaA MCAODC_02665 0.43 +1.0 539,557 - ulaA MCAODC_02665 0.43 +0.5 539,557 - ulaA MCAODC_02665 0.43 -1.6 539,748 + ulaA MCAODC_02665 0.56 -1.2 539,749 - ulaA MCAODC_02665 0.56 +0.2 539,826 + ulaA MCAODC_02665 0.62 -0.4 539,826 + ulaA MCAODC_02665 0.62 +1.3 539,826 + ulaA MCAODC_02665 0.62 -0.2 539,827 - ulaA MCAODC_02665 0.62 +1.2 539,827 - ulaA MCAODC_02665 0.62 +0.4 539,853 + ulaA MCAODC_02665 0.64 -1.4 539,881 + ulaA MCAODC_02665 0.66 +0.3 539,881 + ulaA MCAODC_02665 0.66 -4.7 539,881 + ulaA MCAODC_02665 0.66 +0.4 539,881 + ulaA MCAODC_02665 0.66 -1.3 539,882 - ulaA MCAODC_02665 0.66 +1.3
Or see this region's nucleotide sequence