Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yjfY and ulaF are separated by 128 nucleotides ulaF and ulaE overlap by 1 nucleotides ulaE and ulaD are separated by 9 nucleotides ulaD and ulaC are separated by 13 nucleotides
MCAODC_02635: yjfY - Uncharacterized protein YjfY, at 535,548 to 535,823
yjfY
MCAODC_02640: ulaF - L-ribulose-5-phosphate 4-epimerase UlaF, at 535,952 to 536,638
ulaF
MCAODC_02645: ulaE - L-ribulose-5-phosphate 3-epimerase UlaE, at 536,638 to 537,492
ulaE
MCAODC_02650: ulaD - 3-keto-L-gulonate-6-phosphate decarboxylase UlaD, at 537,502 to 538,152
ulaD
MCAODC_02655: ulaC - PTS ascorbate transporter subunit IIA, at 538,166 to 538,630
ulaC
Position (kb) 536
537
538 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 535.640 kb on + strand, within yjfY at 535.641 kb on - strand, within yjfY at 535.671 kb on + strand, within yjfY at 535.672 kb on - strand, within yjfY at 535.672 kb on - strand, within yjfY at 535.672 kb on - strand, within yjfY at 535.674 kb on - strand, within yjfY at 535.674 kb on - strand, within yjfY at 535.683 kb on - strand, within yjfY at 535.718 kb on - strand, within yjfY at 535.749 kb on + strand, within yjfY at 535.749 kb on + strand, within yjfY at 535.750 kb on - strand, within yjfY at 535.750 kb on - strand, within yjfY at 535.752 kb on + strand, within yjfY at 535.752 kb on + strand, within yjfY at 535.752 kb on + strand, within yjfY at 535.753 kb on - strand, within yjfY at 535.753 kb on - strand, within yjfY at 535.753 kb on - strand, within yjfY at 535.753 kb on - strand, within yjfY at 535.753 kb on - strand, within yjfY at 535.759 kb on + strand, within yjfY at 535.785 kb on - strand, within yjfY at 535.793 kb on + strand, within yjfY at 535.793 kb on + strand, within yjfY at 535.793 kb on + strand, within yjfY at 535.793 kb on + strand, within yjfY at 535.794 kb on - strand, within yjfY at 535.794 kb on - strand, within yjfY at 535.811 kb on + strand at 535.815 kb on + strand at 535.815 kb on + strand at 535.816 kb on - strand at 535.816 kb on - strand at 535.831 kb on + strand at 535.831 kb on + strand at 535.831 kb on + strand at 535.831 kb on + strand at 535.831 kb on + strand at 535.832 kb on - strand at 535.832 kb on - strand at 535.832 kb on - strand at 535.832 kb on - strand at 535.832 kb on - strand at 535.833 kb on + strand at 535.853 kb on + strand at 535.853 kb on + strand at 535.854 kb on - strand at 535.854 kb on - strand at 535.854 kb on - strand at 535.854 kb on - strand at 535.907 kb on + strand at 535.907 kb on + strand at 535.908 kb on - strand at 535.917 kb on - strand at 535.917 kb on - strand at 535.953 kb on + strand at 535.954 kb on - strand at 535.954 kb on - strand at 535.987 kb on + strand at 535.987 kb on + strand at 535.987 kb on + strand at 535.988 kb on - strand at 535.988 kb on - strand at 536.002 kb on + strand at 536.003 kb on - strand at 536.041 kb on + strand, within ulaF at 536.146 kb on - strand, within ulaF at 536.164 kb on + strand, within ulaF at 536.164 kb on + strand, within ulaF at 536.164 kb on + strand, within ulaF at 536.164 kb on + strand, within ulaF at 536.165 kb on - strand, within ulaF at 536.223 kb on + strand, within ulaF at 536.250 kb on - strand, within ulaF at 536.395 kb on - strand, within ulaF at 536.434 kb on - strand, within ulaF at 536.453 kb on - strand, within ulaF at 536.461 kb on + strand, within ulaF at 536.477 kb on - strand, within ulaF at 536.477 kb on - strand, within ulaF at 536.569 kb on + strand, within ulaF at 536.569 kb on + strand, within ulaF at 536.569 kb on + strand, within ulaF at 536.570 kb on - strand at 536.570 kb on - strand at 536.628 kb on - strand at 536.639 kb on + strand at 536.863 kb on + strand, within ulaE at 536.863 kb on + strand, within ulaE at 536.863 kb on + strand, within ulaE at 536.863 kb on + strand, within ulaE at 536.863 kb on + strand, within ulaE at 536.875 kb on - strand, within ulaE at 536.973 kb on - strand, within ulaE at 537.126 kb on - strand, within ulaE at 537.128 kb on + strand, within ulaE at 537.128 kb on + strand, within ulaE at 537.128 kb on + strand, within ulaE at 537.128 kb on + strand, within ulaE at 537.129 kb on - strand, within ulaE at 537.129 kb on - strand, within ulaE at 537.129 kb on - strand, within ulaE at 537.129 kb on - strand, within ulaE at 537.137 kb on + strand, within ulaE at 537.186 kb on + strand, within ulaE at 537.192 kb on + strand, within ulaE at 537.199 kb on - strand, within ulaE at 537.199 kb on - strand, within ulaE at 537.374 kb on - strand, within ulaE at 537.462 kb on - strand at 537.462 kb on - strand at 537.462 kb on - strand at 537.462 kb on - strand at 537.493 kb on - strand at 537.493 kb on - strand at 537.493 kb on - strand at 537.504 kb on - strand at 537.749 kb on + strand, within ulaD at 537.895 kb on + strand, within ulaD at 537.895 kb on + strand, within ulaD at 537.930 kb on + strand, within ulaD at 537.930 kb on + strand, within ulaD at 537.930 kb on + strand, within ulaD at 537.931 kb on - strand, within ulaD at 537.992 kb on + strand, within ulaD at 537.993 kb on - strand, within ulaD at 538.110 kb on + strand at 538.111 kb on - strand at 538.111 kb on - strand at 538.111 kb on - strand at 538.145 kb on - strand at 538.167 kb on + strand at 538.167 kb on + strand at 538.168 kb on - strand at 538.203 kb on - strand at 538.203 kb on - strand at 538.298 kb on - strand, within ulaC at 538.298 kb on - strand, within ulaC at 538.382 kb on + strand, within ulaC at 538.406 kb on - strand, within ulaC at 538.461 kb on + strand, within ulaC at 538.462 kb on - strand, within ulaC at 538.462 kb on - strand, within ulaC
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 535,640 + yjfY MCAODC_02635 0.33 -0.3 535,641 - yjfY MCAODC_02635 0.34 -0.7 535,671 + yjfY MCAODC_02635 0.45 +0.5 535,672 - yjfY MCAODC_02635 0.45 -0.6 535,672 - yjfY MCAODC_02635 0.45 +0.7 535,672 - yjfY MCAODC_02635 0.45 +0.1 535,674 - yjfY MCAODC_02635 0.46 -0.9 535,674 - yjfY MCAODC_02635 0.46 -0.4 535,683 - yjfY MCAODC_02635 0.49 +0.3 535,718 - yjfY MCAODC_02635 0.62 +0.6 535,749 + yjfY MCAODC_02635 0.73 +0.3 535,749 + yjfY MCAODC_02635 0.73 +0.2 535,750 - yjfY MCAODC_02635 0.73 +0.3 535,750 - yjfY MCAODC_02635 0.73 +0.8 535,752 + yjfY MCAODC_02635 0.74 +1.7 535,752 + yjfY MCAODC_02635 0.74 -2.3 535,752 + yjfY MCAODC_02635 0.74 +1.8 535,753 - yjfY MCAODC_02635 0.74 -1.1 535,753 - yjfY MCAODC_02635 0.74 +0.6 535,753 - yjfY MCAODC_02635 0.74 -0.8 535,753 - yjfY MCAODC_02635 0.74 +0.1 535,753 - yjfY MCAODC_02635 0.74 +0.1 535,759 + yjfY MCAODC_02635 0.76 +0.8 535,785 - yjfY MCAODC_02635 0.86 +0.1 535,793 + yjfY MCAODC_02635 0.89 -0.7 535,793 + yjfY MCAODC_02635 0.89 +1.3 535,793 + yjfY MCAODC_02635 0.89 -0.1 535,793 + yjfY MCAODC_02635 0.89 -0.1 535,794 - yjfY MCAODC_02635 0.89 -0.6 535,794 - yjfY MCAODC_02635 0.89 +0.8 535,811 + +0.2 535,815 + +1.1 535,815 + -0.4 535,816 - -1.1 535,816 - -0.0 535,831 + -0.8 535,831 + -0.4 535,831 + +1.2 535,831 + -0.2 535,831 + -1.6 535,832 - +0.8 535,832 - +1.7 535,832 - +1.2 535,832 - +0.2 535,832 - +1.5 535,833 + +0.3 535,853 + +0.9 535,853 + -0.6 535,854 - +0.9 535,854 - +0.2 535,854 - +0.4 535,854 - -4.2 535,907 + -1.1 535,907 + -0.9 535,908 - +0.5 535,917 - -2.2 535,917 - -1.1 535,953 + -0.3 535,954 - +0.8 535,954 - +0.3 535,987 + -1.5 535,987 + +1.4 535,987 + +0.8 535,988 - +0.2 535,988 - +0.1 536,002 + -3.9 536,003 - +0.0 536,041 + ulaF MCAODC_02640 0.13 -1.0 536,146 - ulaF MCAODC_02640 0.28 +0.0 536,164 + ulaF MCAODC_02640 0.31 -0.4 536,164 + ulaF MCAODC_02640 0.31 -0.9 536,164 + ulaF MCAODC_02640 0.31 -1.6 536,164 + ulaF MCAODC_02640 0.31 -1.7 536,165 - ulaF MCAODC_02640 0.31 -0.1 536,223 + ulaF MCAODC_02640 0.39 +0.7 536,250 - ulaF MCAODC_02640 0.43 +0.1 536,395 - ulaF MCAODC_02640 0.64 +0.3 536,434 - ulaF MCAODC_02640 0.70 +0.4 536,453 - ulaF MCAODC_02640 0.73 -0.3 536,461 + ulaF MCAODC_02640 0.74 -1.8 536,477 - ulaF MCAODC_02640 0.76 +0.3 536,477 - ulaF MCAODC_02640 0.76 +1.6 536,569 + ulaF MCAODC_02640 0.90 +2.1 536,569 + ulaF MCAODC_02640 0.90 -0.4 536,569 + ulaF MCAODC_02640 0.90 -0.3 536,570 - -0.5 536,570 - -0.1 536,628 - +1.0 536,639 + -0.0 536,863 + ulaE MCAODC_02645 0.26 -0.2 536,863 + ulaE MCAODC_02645 0.26 -0.3 536,863 + ulaE MCAODC_02645 0.26 -1.2 536,863 + ulaE MCAODC_02645 0.26 -0.4 536,863 + ulaE MCAODC_02645 0.26 -0.4 536,875 - ulaE MCAODC_02645 0.28 +1.1 536,973 - ulaE MCAODC_02645 0.39 -0.4 537,126 - ulaE MCAODC_02645 0.57 -0.2 537,128 + ulaE MCAODC_02645 0.57 -0.3 537,128 + ulaE MCAODC_02645 0.57 -0.0 537,128 + ulaE MCAODC_02645 0.57 +1.0 537,128 + ulaE MCAODC_02645 0.57 -0.7 537,129 - ulaE MCAODC_02645 0.57 -0.3 537,129 - ulaE MCAODC_02645 0.57 +0.8 537,129 - ulaE MCAODC_02645 0.57 +0.3 537,129 - ulaE MCAODC_02645 0.57 +0.7 537,137 + ulaE MCAODC_02645 0.58 -1.0 537,186 + ulaE MCAODC_02645 0.64 -0.9 537,192 + ulaE MCAODC_02645 0.65 -0.9 537,199 - ulaE MCAODC_02645 0.66 -0.1 537,199 - ulaE MCAODC_02645 0.66 -1.9 537,374 - ulaE MCAODC_02645 0.86 -0.0 537,462 - +0.2 537,462 - +0.2 537,462 - +0.8 537,462 - +0.6 537,493 - +1.1 537,493 - +0.4 537,493 - -1.1 537,504 - +0.2 537,749 + ulaD MCAODC_02650 0.38 -0.1 537,895 + ulaD MCAODC_02650 0.60 -0.3 537,895 + ulaD MCAODC_02650 0.60 +0.4 537,930 + ulaD MCAODC_02650 0.66 -0.3 537,930 + ulaD MCAODC_02650 0.66 +1.1 537,930 + ulaD MCAODC_02650 0.66 -0.4 537,931 - ulaD MCAODC_02650 0.66 +1.0 537,992 + ulaD MCAODC_02650 0.75 -0.9 537,993 - ulaD MCAODC_02650 0.75 -0.6 538,110 + -0.1 538,111 - +1.6 538,111 - -0.1 538,111 - +1.6 538,145 - +0.7 538,167 + +0.2 538,167 + +0.0 538,168 - +0.4 538,203 - +0.8 538,203 - -0.0 538,298 - ulaC MCAODC_02655 0.28 -0.3 538,298 - ulaC MCAODC_02655 0.28 -0.1 538,382 + ulaC MCAODC_02655 0.46 -0.4 538,406 - ulaC MCAODC_02655 0.52 -0.9 538,461 + ulaC MCAODC_02655 0.63 -0.4 538,462 - ulaC MCAODC_02655 0.64 -0.4 538,462 - ulaC MCAODC_02655 0.64 -2.1
Or see this region's nucleotide sequence