Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nrdG and cybC are separated by 44 nucleotides cybC and pmbA are separated by 183 nucleotides pmbA and yjgA are separated by 93 nucleotides yjgA and mpl are separated by 155 nucleotides
MCAODC_02420: nrdG - anaerobic ribonucleoside-triphosphate reductase-activating protein, at 498,212 to 498,676
nrdG
MCAODC_02425: cybC - cytochrome b562, at 498,721 to 499,107
cybC
MCAODC_02430: pmbA - metalloprotease PmbA, at 499,291 to 500,643
pmbA
MCAODC_02435: yjgA - ribosome-associated protein, at 500,737 to 501,288
yjgA
MCAODC_02445: mpl - UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-me so-diaminopimelate ligase, at 501,444 to 502,817
mpl
Position (kb) 499
500
501 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 498.311 kb on + strand, within nrdG at 498.311 kb on + strand, within nrdG at 498.311 kb on + strand, within nrdG at 498.311 kb on + strand, within nrdG at 498.312 kb on - strand, within nrdG at 498.312 kb on - strand, within nrdG at 498.312 kb on - strand, within nrdG at 498.312 kb on - strand, within nrdG at 498.312 kb on - strand, within nrdG at 498.313 kb on + strand, within nrdG at 498.313 kb on + strand, within nrdG at 498.352 kb on + strand, within nrdG at 498.353 kb on - strand, within nrdG at 498.356 kb on - strand, within nrdG at 498.401 kb on - strand, within nrdG at 498.416 kb on - strand, within nrdG at 498.477 kb on + strand, within nrdG at 498.530 kb on + strand, within nrdG at 498.677 kb on + strand at 498.677 kb on + strand at 498.677 kb on + strand at 498.677 kb on + strand at 498.677 kb on + strand at 498.678 kb on - strand at 498.678 kb on - strand at 498.678 kb on - strand at 498.722 kb on + strand at 498.722 kb on + strand at 498.729 kb on - strand at 498.729 kb on - strand at 498.729 kb on - strand at 498.790 kb on + strand, within cybC at 498.791 kb on - strand, within cybC at 498.796 kb on - strand, within cybC at 498.891 kb on + strand, within cybC at 498.892 kb on - strand, within cybC at 499.000 kb on + strand, within cybC at 499.064 kb on - strand, within cybC at 499.086 kb on + strand at 499.086 kb on + strand at 499.086 kb on + strand at 499.086 kb on + strand at 499.086 kb on + strand at 499.087 kb on - strand at 499.087 kb on - strand at 499.090 kb on + strand at 499.090 kb on + strand at 499.091 kb on - strand at 499.091 kb on - strand at 499.124 kb on + strand at 499.128 kb on + strand at 499.129 kb on - strand at 499.147 kb on + strand at 499.148 kb on - strand at 499.148 kb on - strand at 499.148 kb on - strand at 499.163 kb on - strand at 499.185 kb on + strand at 499.186 kb on - strand at 499.186 kb on - strand at 499.194 kb on + strand at 499.195 kb on - strand at 499.263 kb on + strand at 499.263 kb on + strand at 499.264 kb on - strand at 499.264 kb on - strand at 499.264 kb on - strand at 499.264 kb on - strand at 499.266 kb on - strand at 499.339 kb on + strand at 499.339 kb on + strand at 499.339 kb on + strand at 499.339 kb on + strand at 499.340 kb on - strand at 499.340 kb on - strand at 499.340 kb on - strand at 499.340 kb on - strand at 499.340 kb on - strand at 499.340 kb on - strand at 499.340 kb on - strand at 499.340 kb on - strand at 499.340 kb on - strand at 499.340 kb on - strand at 499.340 kb on - strand at 499.342 kb on - strand at 499.342 kb on - strand at 499.392 kb on + strand at 499.393 kb on - strand at 499.393 kb on - strand at 499.400 kb on - strand at 499.400 kb on - strand at 499.481 kb on + strand, within pmbA at 499.570 kb on + strand, within pmbA at 499.571 kb on - strand, within pmbA at 499.571 kb on - strand, within pmbA at 499.571 kb on - strand, within pmbA at 499.571 kb on - strand, within pmbA at 499.603 kb on - strand, within pmbA at 499.649 kb on + strand, within pmbA at 499.650 kb on - strand, within pmbA at 499.686 kb on + strand, within pmbA at 499.693 kb on - strand, within pmbA at 499.830 kb on + strand, within pmbA at 499.830 kb on + strand, within pmbA at 499.830 kb on + strand, within pmbA at 499.830 kb on + strand, within pmbA at 499.836 kb on - strand, within pmbA at 499.932 kb on + strand, within pmbA at 499.951 kb on + strand, within pmbA at 500.024 kb on + strand, within pmbA at 500.030 kb on + strand, within pmbA at 500.065 kb on + strand, within pmbA at 500.066 kb on - strand, within pmbA at 500.066 kb on - strand, within pmbA at 500.066 kb on - strand, within pmbA at 500.337 kb on + strand, within pmbA at 500.337 kb on + strand, within pmbA at 500.337 kb on + strand, within pmbA at 500.338 kb on - strand, within pmbA at 500.339 kb on + strand, within pmbA at 500.340 kb on - strand, within pmbA at 500.366 kb on - strand, within pmbA at 500.413 kb on - strand, within pmbA at 500.432 kb on + strand, within pmbA at 500.433 kb on - strand, within pmbA at 500.490 kb on - strand, within pmbA at 500.501 kb on - strand, within pmbA at 500.501 kb on - strand, within pmbA at 500.506 kb on - strand, within pmbA at 500.623 kb on + strand at 500.623 kb on + strand at 500.624 kb on - strand at 500.624 kb on - strand at 500.643 kb on + strand at 500.643 kb on + strand at 500.644 kb on - strand at 500.648 kb on + strand at 500.649 kb on - strand at 500.669 kb on - strand at 500.698 kb on + strand at 500.699 kb on - strand at 500.699 kb on - strand at 500.699 kb on - strand at 500.699 kb on - strand at 500.712 kb on - strand at 500.742 kb on + strand at 500.742 kb on + strand at 500.742 kb on + strand at 500.743 kb on - strand at 500.743 kb on - strand at 500.808 kb on + strand, within yjgA at 500.808 kb on + strand, within yjgA at 500.821 kb on - strand, within yjgA at 500.821 kb on - strand, within yjgA at 500.852 kb on + strand, within yjgA at 500.852 kb on + strand, within yjgA at 500.852 kb on + strand, within yjgA at 500.853 kb on - strand, within yjgA at 500.912 kb on + strand, within yjgA at 500.955 kb on + strand, within yjgA at 500.955 kb on + strand, within yjgA at 500.955 kb on + strand, within yjgA at 500.956 kb on - strand, within yjgA at 501.017 kb on + strand, within yjgA at 501.018 kb on - strand, within yjgA at 501.084 kb on + strand, within yjgA at 501.084 kb on + strand, within yjgA at 501.085 kb on - strand, within yjgA at 501.146 kb on + strand, within yjgA at 501.147 kb on - strand, within yjgA at 501.147 kb on - strand, within yjgA at 501.147 kb on - strand, within yjgA at 501.222 kb on + strand, within yjgA at 501.223 kb on - strand, within yjgA at 501.256 kb on + strand at 501.257 kb on - strand at 501.257 kb on - strand at 501.287 kb on - strand at 501.308 kb on + strand at 501.309 kb on - strand at 501.309 kb on - strand at 501.336 kb on - strand at 501.345 kb on - strand at 501.374 kb on - strand at 501.374 kb on - strand at 501.374 kb on - strand at 501.424 kb on + strand at 501.425 kb on - strand at 501.425 kb on - strand at 501.483 kb on + strand at 501.530 kb on + strand at 501.531 kb on - strand at 501.563 kb on + strand at 501.564 kb on - strand at 501.564 kb on - strand at 501.564 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 498,311 + nrdG MCAODC_02420 0.21 +0.0 498,311 + nrdG MCAODC_02420 0.21 +1.7 498,311 + nrdG MCAODC_02420 0.21 -0.2 498,311 + nrdG MCAODC_02420 0.21 -0.3 498,312 - nrdG MCAODC_02420 0.22 +0.5 498,312 - nrdG MCAODC_02420 0.22 +0.4 498,312 - nrdG MCAODC_02420 0.22 -0.9 498,312 - nrdG MCAODC_02420 0.22 -0.3 498,312 - nrdG MCAODC_02420 0.22 +0.1 498,313 + nrdG MCAODC_02420 0.22 +0.2 498,313 + nrdG MCAODC_02420 0.22 +1.7 498,352 + nrdG MCAODC_02420 0.30 +0.5 498,353 - nrdG MCAODC_02420 0.30 -0.5 498,356 - nrdG MCAODC_02420 0.31 +0.0 498,401 - nrdG MCAODC_02420 0.41 +0.9 498,416 - nrdG MCAODC_02420 0.44 -0.4 498,477 + nrdG MCAODC_02420 0.57 -2.1 498,530 + nrdG MCAODC_02420 0.68 +1.7 498,677 + -3.2 498,677 + +1.7 498,677 + +0.4 498,677 + +0.5 498,677 + -1.3 498,678 - +0.2 498,678 - -0.9 498,678 - +0.5 498,722 + +0.1 498,722 + +0.0 498,729 - +2.0 498,729 - -0.1 498,729 - -0.1 498,790 + cybC MCAODC_02425 0.18 -0.8 498,791 - cybC MCAODC_02425 0.18 +0.3 498,796 - cybC MCAODC_02425 0.19 +0.5 498,891 + cybC MCAODC_02425 0.44 -0.1 498,892 - cybC MCAODC_02425 0.44 -0.7 499,000 + cybC MCAODC_02425 0.72 -0.4 499,064 - cybC MCAODC_02425 0.89 -0.2 499,086 + +0.6 499,086 + +0.4 499,086 + +1.4 499,086 + +0.2 499,086 + -0.1 499,087 - +1.0 499,087 - -0.3 499,090 + +0.4 499,090 + -0.6 499,091 - +0.2 499,091 - +0.2 499,124 + -0.1 499,128 + +1.5 499,129 - -0.1 499,147 + +0.7 499,148 - -1.5 499,148 - +1.4 499,148 - -0.6 499,163 - -0.8 499,185 + +0.3 499,186 - +0.1 499,186 - +0.0 499,194 + +0.3 499,195 - -1.5 499,263 + -0.9 499,263 + -1.4 499,264 - +0.7 499,264 - -0.5 499,264 - -0.9 499,264 - +1.6 499,266 - -0.4 499,339 + -0.1 499,339 + -0.1 499,339 + +0.4 499,339 + +1.3 499,340 - -1.9 499,340 - +0.5 499,340 - +0.7 499,340 - +1.1 499,340 - +0.5 499,340 - +0.4 499,340 - -1.0 499,340 - -1.5 499,340 - -0.3 499,340 - +0.4 499,340 - +1.2 499,342 - -0.6 499,342 - +1.1 499,392 + -0.2 499,393 - +1.2 499,393 - -0.1 499,400 - -2.2 499,400 - -1.2 499,481 + pmbA MCAODC_02430 0.14 +0.7 499,570 + pmbA MCAODC_02430 0.21 +2.2 499,571 - pmbA MCAODC_02430 0.21 +0.1 499,571 - pmbA MCAODC_02430 0.21 +0.1 499,571 - pmbA MCAODC_02430 0.21 -0.1 499,571 - pmbA MCAODC_02430 0.21 -0.3 499,603 - pmbA MCAODC_02430 0.23 -1.0 499,649 + pmbA MCAODC_02430 0.26 +0.3 499,650 - pmbA MCAODC_02430 0.27 +0.9 499,686 + pmbA MCAODC_02430 0.29 +1.3 499,693 - pmbA MCAODC_02430 0.30 +0.7 499,830 + pmbA MCAODC_02430 0.40 +0.1 499,830 + pmbA MCAODC_02430 0.40 +0.7 499,830 + pmbA MCAODC_02430 0.40 +0.4 499,830 + pmbA MCAODC_02430 0.40 +0.0 499,836 - pmbA MCAODC_02430 0.40 -1.3 499,932 + pmbA MCAODC_02430 0.47 -1.5 499,951 + pmbA MCAODC_02430 0.49 +0.7 500,024 + pmbA MCAODC_02430 0.54 -0.3 500,030 + pmbA MCAODC_02430 0.55 +2.4 500,065 + pmbA MCAODC_02430 0.57 +0.6 500,066 - pmbA MCAODC_02430 0.57 +0.4 500,066 - pmbA MCAODC_02430 0.57 -0.8 500,066 - pmbA MCAODC_02430 0.57 +0.0 500,337 + pmbA MCAODC_02430 0.77 +0.0 500,337 + pmbA MCAODC_02430 0.77 +0.7 500,337 + pmbA MCAODC_02430 0.77 +1.5 500,338 - pmbA MCAODC_02430 0.77 -1.1 500,339 + pmbA MCAODC_02430 0.77 +2.0 500,340 - pmbA MCAODC_02430 0.78 -0.8 500,366 - pmbA MCAODC_02430 0.79 -0.3 500,413 - pmbA MCAODC_02430 0.83 -0.4 500,432 + pmbA MCAODC_02430 0.84 -0.1 500,433 - pmbA MCAODC_02430 0.84 -0.6 500,490 - pmbA MCAODC_02430 0.89 -0.6 500,501 - pmbA MCAODC_02430 0.89 +1.2 500,501 - pmbA MCAODC_02430 0.89 -0.1 500,506 - pmbA MCAODC_02430 0.90 +2.2 500,623 + +0.4 500,623 + +0.1 500,624 - +1.8 500,624 - +0.3 500,643 + +0.3 500,643 + -0.9 500,644 - +0.2 500,648 + +0.6 500,649 - +0.3 500,669 - -0.3 500,698 + -0.9 500,699 - +2.3 500,699 - -0.0 500,699 - +0.1 500,699 - +0.8 500,712 - +0.5 500,742 + -0.3 500,742 + -0.3 500,742 + +0.5 500,743 - +0.5 500,743 - +0.2 500,808 + yjgA MCAODC_02435 0.13 +0.8 500,808 + yjgA MCAODC_02435 0.13 -0.8 500,821 - yjgA MCAODC_02435 0.15 -2.0 500,821 - yjgA MCAODC_02435 0.15 -1.6 500,852 + yjgA MCAODC_02435 0.21 -2.3 500,852 + yjgA MCAODC_02435 0.21 +0.5 500,852 + yjgA MCAODC_02435 0.21 +0.1 500,853 - yjgA MCAODC_02435 0.21 -1.3 500,912 + yjgA MCAODC_02435 0.32 +0.6 500,955 + yjgA MCAODC_02435 0.39 +0.0 500,955 + yjgA MCAODC_02435 0.39 +1.6 500,955 + yjgA MCAODC_02435 0.39 -0.6 500,956 - yjgA MCAODC_02435 0.40 -1.5 501,017 + yjgA MCAODC_02435 0.51 -1.2 501,018 - yjgA MCAODC_02435 0.51 -1.5 501,084 + yjgA MCAODC_02435 0.63 +0.9 501,084 + yjgA MCAODC_02435 0.63 -1.0 501,085 - yjgA MCAODC_02435 0.63 +0.9 501,146 + yjgA MCAODC_02435 0.74 -1.3 501,147 - yjgA MCAODC_02435 0.74 -1.8 501,147 - yjgA MCAODC_02435 0.74 -0.1 501,147 - yjgA MCAODC_02435 0.74 +0.5 501,222 + yjgA MCAODC_02435 0.88 -0.1 501,223 - yjgA MCAODC_02435 0.88 -0.7 501,256 + +0.7 501,257 - +0.5 501,257 - -0.7 501,287 - +0.4 501,308 + +1.5 501,309 - -0.0 501,309 - -0.3 501,336 - +0.9 501,345 - +1.2 501,374 - -0.4 501,374 - +0.8 501,374 - -0.7 501,424 + +1.0 501,425 - +0.4 501,425 - +0.2 501,483 + +0.8 501,530 + +0.9 501,531 - +0.2 501,563 + -0.1 501,564 - +1.3 501,564 - +0.2 501,564 - -0.8
Or see this region's nucleotide sequence