Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt treC and nrdD are separated by 393 nucleotides nrdD and nrdG are separated by 189 nucleotides nrdG and cybC are separated by 44 nucleotides
MCAODC_02405: treC - alpha,alpha-phosphotrehalase, at 493,835 to 495,490
treC
MCAODC_02410: nrdD - anaerobic ribonucleoside-triphosphate reductase, at 495,884 to 498,022
nrdD
MCAODC_02420: nrdG - anaerobic ribonucleoside-triphosphate reductase-activating protein, at 498,212 to 498,676
nrdG
MCAODC_02425: cybC - cytochrome b562, at 498,721 to 499,107
cybC
Position (kb) 495
496
497
498
499 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 495.168 kb on + strand, within treC at 495.412 kb on + strand at 495.488 kb on + strand at 495.488 kb on + strand at 495.489 kb on - strand at 495.593 kb on + strand at 495.594 kb on - strand at 495.594 kb on - strand at 495.594 kb on - strand at 495.602 kb on - strand at 495.618 kb on - strand at 495.641 kb on + strand at 495.641 kb on + strand at 495.642 kb on - strand at 495.680 kb on + strand at 495.682 kb on + strand at 495.682 kb on + strand at 495.683 kb on - strand at 495.683 kb on - strand at 495.685 kb on - strand at 495.731 kb on + strand at 495.733 kb on + strand at 495.734 kb on - strand at 495.734 kb on - strand at 495.734 kb on - strand at 495.734 kb on - strand at 495.743 kb on + strand at 495.743 kb on + strand at 495.743 kb on + strand at 495.744 kb on - strand at 495.744 kb on - strand at 495.872 kb on + strand at 495.872 kb on + strand at 495.873 kb on - strand at 495.928 kb on + strand at 495.967 kb on + strand at 495.968 kb on - strand at 495.968 kb on - strand at 495.968 kb on - strand at 495.968 kb on - strand at 495.996 kb on - strand at 496.103 kb on + strand, within nrdD at 496.103 kb on + strand, within nrdD at 496.124 kb on + strand, within nrdD at 496.124 kb on + strand, within nrdD at 496.124 kb on + strand, within nrdD at 496.124 kb on + strand, within nrdD at 496.125 kb on - strand, within nrdD at 496.125 kb on - strand, within nrdD at 496.125 kb on - strand, within nrdD at 496.301 kb on + strand, within nrdD at 496.301 kb on + strand, within nrdD at 496.302 kb on - strand, within nrdD at 496.302 kb on - strand, within nrdD at 496.379 kb on + strand, within nrdD at 496.379 kb on + strand, within nrdD at 496.380 kb on - strand, within nrdD at 496.380 kb on - strand, within nrdD at 496.447 kb on + strand, within nrdD at 496.448 kb on - strand, within nrdD at 496.448 kb on - strand, within nrdD at 496.517 kb on - strand, within nrdD at 496.537 kb on + strand, within nrdD at 496.537 kb on + strand, within nrdD at 496.537 kb on + strand, within nrdD at 496.537 kb on + strand, within nrdD at 496.538 kb on - strand, within nrdD at 496.538 kb on - strand, within nrdD at 496.538 kb on - strand, within nrdD at 496.564 kb on + strand, within nrdD at 496.601 kb on + strand, within nrdD at 496.602 kb on - strand, within nrdD at 496.656 kb on - strand, within nrdD at 496.660 kb on + strand, within nrdD at 496.660 kb on + strand, within nrdD at 496.685 kb on + strand, within nrdD at 496.685 kb on + strand, within nrdD at 496.685 kb on + strand, within nrdD at 496.686 kb on - strand, within nrdD at 496.686 kb on - strand, within nrdD at 496.716 kb on + strand, within nrdD at 496.716 kb on + strand, within nrdD at 496.716 kb on + strand, within nrdD at 496.717 kb on - strand, within nrdD at 496.717 kb on - strand, within nrdD at 496.717 kb on - strand, within nrdD at 496.810 kb on + strand, within nrdD at 496.811 kb on - strand, within nrdD at 496.811 kb on - strand, within nrdD at 496.923 kb on - strand, within nrdD at 496.923 kb on - strand, within nrdD at 496.923 kb on - strand, within nrdD at 496.923 kb on - strand, within nrdD at 496.968 kb on - strand, within nrdD at 496.968 kb on - strand, within nrdD at 496.986 kb on - strand, within nrdD at 496.986 kb on - strand, within nrdD at 496.995 kb on + strand, within nrdD at 496.996 kb on - strand, within nrdD at 497.093 kb on - strand, within nrdD at 497.200 kb on + strand, within nrdD at 497.200 kb on + strand, within nrdD at 497.200 kb on + strand, within nrdD at 497.264 kb on + strand, within nrdD at 497.264 kb on + strand, within nrdD at 497.264 kb on + strand, within nrdD at 497.264 kb on + strand, within nrdD at 497.264 kb on + strand, within nrdD at 497.265 kb on - strand, within nrdD at 497.265 kb on - strand, within nrdD at 497.265 kb on - strand, within nrdD at 497.266 kb on + strand, within nrdD at 497.266 kb on + strand, within nrdD at 497.266 kb on + strand, within nrdD at 497.267 kb on - strand, within nrdD at 497.308 kb on + strand, within nrdD at 497.309 kb on - strand, within nrdD at 497.341 kb on + strand, within nrdD at 497.354 kb on + strand, within nrdD at 497.354 kb on + strand, within nrdD at 497.406 kb on - strand, within nrdD at 497.597 kb on + strand, within nrdD at 497.597 kb on + strand, within nrdD at 497.597 kb on + strand, within nrdD at 497.598 kb on - strand, within nrdD at 497.598 kb on - strand, within nrdD at 497.598 kb on - strand, within nrdD at 497.600 kb on + strand, within nrdD at 497.600 kb on + strand, within nrdD at 497.600 kb on + strand, within nrdD at 497.600 kb on + strand, within nrdD at 497.600 kb on + strand, within nrdD at 497.600 kb on + strand, within nrdD at 497.600 kb on + strand, within nrdD at 497.601 kb on - strand, within nrdD at 497.673 kb on - strand, within nrdD at 497.715 kb on - strand, within nrdD at 497.762 kb on + strand, within nrdD at 497.762 kb on + strand, within nrdD at 497.763 kb on - strand, within nrdD at 497.768 kb on + strand, within nrdD at 497.778 kb on + strand, within nrdD at 497.778 kb on + strand, within nrdD at 497.778 kb on + strand, within nrdD at 497.779 kb on - strand, within nrdD at 497.779 kb on - strand, within nrdD at 497.783 kb on + strand, within nrdD at 497.784 kb on - strand, within nrdD at 497.784 kb on - strand, within nrdD at 497.797 kb on + strand, within nrdD at 497.798 kb on - strand, within nrdD at 497.798 kb on - strand, within nrdD at 497.798 kb on - strand, within nrdD at 497.798 kb on - strand, within nrdD at 497.816 kb on + strand at 497.817 kb on - strand at 497.817 kb on - strand at 497.907 kb on + strand at 497.907 kb on + strand at 497.931 kb on + strand at 497.931 kb on + strand at 497.931 kb on + strand at 497.931 kb on + strand at 497.931 kb on + strand at 497.932 kb on - strand at 497.932 kb on - strand at 497.932 kb on - strand at 497.932 kb on - strand at 497.932 kb on - strand at 497.956 kb on + strand at 497.981 kb on - strand at 497.981 kb on - strand at 498.015 kb on + strand at 498.016 kb on - strand at 498.016 kb on - strand at 498.030 kb on + strand at 498.030 kb on + strand at 498.030 kb on + strand at 498.128 kb on - strand at 498.183 kb on - strand at 498.211 kb on - strand at 498.211 kb on - strand at 498.211 kb on - strand at 498.218 kb on + strand at 498.218 kb on + strand at 498.219 kb on - strand at 498.219 kb on - strand at 498.227 kb on + strand at 498.227 kb on + strand at 498.227 kb on + strand at 498.227 kb on + strand at 498.227 kb on + strand at 498.228 kb on - strand at 498.228 kb on - strand at 498.228 kb on - strand at 498.228 kb on - strand at 498.228 kb on - strand at 498.230 kb on + strand at 498.230 kb on + strand at 498.231 kb on - strand at 498.231 kb on - strand at 498.311 kb on + strand, within nrdG at 498.311 kb on + strand, within nrdG at 498.311 kb on + strand, within nrdG at 498.311 kb on + strand, within nrdG at 498.312 kb on - strand, within nrdG at 498.312 kb on - strand, within nrdG at 498.312 kb on - strand, within nrdG at 498.312 kb on - strand, within nrdG at 498.312 kb on - strand, within nrdG at 498.313 kb on + strand, within nrdG at 498.313 kb on + strand, within nrdG at 498.352 kb on + strand, within nrdG at 498.353 kb on - strand, within nrdG at 498.356 kb on - strand, within nrdG at 498.401 kb on - strand, within nrdG at 498.416 kb on - strand, within nrdG at 498.477 kb on + strand, within nrdG at 498.530 kb on + strand, within nrdG at 498.677 kb on + strand at 498.677 kb on + strand at 498.677 kb on + strand at 498.677 kb on + strand at 498.677 kb on + strand at 498.678 kb on - strand at 498.678 kb on - strand at 498.678 kb on - strand at 498.722 kb on + strand at 498.722 kb on + strand at 498.729 kb on - strand at 498.729 kb on - strand at 498.729 kb on - strand at 498.790 kb on + strand, within cybC at 498.791 kb on - strand, within cybC at 498.796 kb on - strand, within cybC at 498.891 kb on + strand, within cybC at 498.892 kb on - strand, within cybC at 499.000 kb on + strand, within cybC
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 495,168 + treC MCAODC_02405 0.80 -0.9 495,412 + -1.5 495,488 + -1.4 495,488 + +0.0 495,489 - -0.3 495,593 + -1.8 495,594 - +0.5 495,594 - -0.7 495,594 - +0.1 495,602 - -0.3 495,618 - +0.1 495,641 + -0.3 495,641 + -0.3 495,642 - -0.8 495,680 + -0.3 495,682 + +0.6 495,682 + -0.9 495,683 - +0.9 495,683 - +2.4 495,685 - -2.8 495,731 + +1.1 495,733 + +0.5 495,734 - +0.3 495,734 - +0.4 495,734 - +0.8 495,734 - -3.2 495,743 + -2.2 495,743 + -0.4 495,743 + -1.9 495,744 - -0.2 495,744 - -0.9 495,872 + -0.7 495,872 + +0.4 495,873 - +0.1 495,928 + -0.6 495,967 + -0.5 495,968 - +0.8 495,968 - -0.0 495,968 - -2.3 495,968 - -0.1 495,996 - +0.2 496,103 + nrdD MCAODC_02410 0.10 +0.4 496,103 + nrdD MCAODC_02410 0.10 -0.8 496,124 + nrdD MCAODC_02410 0.11 -0.4 496,124 + nrdD MCAODC_02410 0.11 +0.0 496,124 + nrdD MCAODC_02410 0.11 +0.2 496,124 + nrdD MCAODC_02410 0.11 +0.1 496,125 - nrdD MCAODC_02410 0.11 +0.4 496,125 - nrdD MCAODC_02410 0.11 +0.0 496,125 - nrdD MCAODC_02410 0.11 -0.1 496,301 + nrdD MCAODC_02410 0.19 +0.4 496,301 + nrdD MCAODC_02410 0.19 +0.5 496,302 - nrdD MCAODC_02410 0.20 +0.4 496,302 - nrdD MCAODC_02410 0.20 -0.9 496,379 + nrdD MCAODC_02410 0.23 +0.7 496,379 + nrdD MCAODC_02410 0.23 +0.5 496,380 - nrdD MCAODC_02410 0.23 -2.1 496,380 - nrdD MCAODC_02410 0.23 -1.9 496,447 + nrdD MCAODC_02410 0.26 +1.0 496,448 - nrdD MCAODC_02410 0.26 -1.1 496,448 - nrdD MCAODC_02410 0.26 +0.1 496,517 - nrdD MCAODC_02410 0.30 +1.6 496,537 + nrdD MCAODC_02410 0.31 +0.5 496,537 + nrdD MCAODC_02410 0.31 -0.0 496,537 + nrdD MCAODC_02410 0.31 -0.5 496,537 + nrdD MCAODC_02410 0.31 -0.1 496,538 - nrdD MCAODC_02410 0.31 +0.6 496,538 - nrdD MCAODC_02410 0.31 +0.2 496,538 - nrdD MCAODC_02410 0.31 -0.4 496,564 + nrdD MCAODC_02410 0.32 -2.7 496,601 + nrdD MCAODC_02410 0.34 +1.9 496,602 - nrdD MCAODC_02410 0.34 -1.9 496,656 - nrdD MCAODC_02410 0.36 +0.8 496,660 + nrdD MCAODC_02410 0.36 +0.2 496,660 + nrdD MCAODC_02410 0.36 +1.2 496,685 + nrdD MCAODC_02410 0.37 -0.1 496,685 + nrdD MCAODC_02410 0.37 -0.2 496,685 + nrdD MCAODC_02410 0.37 +2.4 496,686 - nrdD MCAODC_02410 0.37 -2.8 496,686 - nrdD MCAODC_02410 0.37 +1.7 496,716 + nrdD MCAODC_02410 0.39 +2.0 496,716 + nrdD MCAODC_02410 0.39 -0.5 496,716 + nrdD MCAODC_02410 0.39 +0.0 496,717 - nrdD MCAODC_02410 0.39 -2.8 496,717 - nrdD MCAODC_02410 0.39 +0.3 496,717 - nrdD MCAODC_02410 0.39 -2.1 496,810 + nrdD MCAODC_02410 0.43 +0.2 496,811 - nrdD MCAODC_02410 0.43 +1.1 496,811 - nrdD MCAODC_02410 0.43 -3.3 496,923 - nrdD MCAODC_02410 0.49 +1.7 496,923 - nrdD MCAODC_02410 0.49 -0.3 496,923 - nrdD MCAODC_02410 0.49 +0.7 496,923 - nrdD MCAODC_02410 0.49 -0.2 496,968 - nrdD MCAODC_02410 0.51 -1.1 496,968 - nrdD MCAODC_02410 0.51 +0.5 496,986 - nrdD MCAODC_02410 0.52 +0.9 496,986 - nrdD MCAODC_02410 0.52 +0.0 496,995 + nrdD MCAODC_02410 0.52 -0.8 496,996 - nrdD MCAODC_02410 0.52 +0.5 497,093 - nrdD MCAODC_02410 0.57 +0.8 497,200 + nrdD MCAODC_02410 0.62 -0.3 497,200 + nrdD MCAODC_02410 0.62 -0.6 497,200 + nrdD MCAODC_02410 0.62 +0.3 497,264 + nrdD MCAODC_02410 0.65 +0.7 497,264 + nrdD MCAODC_02410 0.65 +0.0 497,264 + nrdD MCAODC_02410 0.65 -0.1 497,264 + nrdD MCAODC_02410 0.65 +0.5 497,264 + nrdD MCAODC_02410 0.65 +2.2 497,265 - nrdD MCAODC_02410 0.65 +1.1 497,265 - nrdD MCAODC_02410 0.65 +1.1 497,265 - nrdD MCAODC_02410 0.65 +0.2 497,266 + nrdD MCAODC_02410 0.65 -0.1 497,266 + nrdD MCAODC_02410 0.65 +0.3 497,266 + nrdD MCAODC_02410 0.65 +1.7 497,267 - nrdD MCAODC_02410 0.65 -0.5 497,308 + nrdD MCAODC_02410 0.67 -0.8 497,309 - nrdD MCAODC_02410 0.67 -1.4 497,341 + nrdD MCAODC_02410 0.68 +0.5 497,354 + nrdD MCAODC_02410 0.69 +0.8 497,354 + nrdD MCAODC_02410 0.69 -0.7 497,406 - nrdD MCAODC_02410 0.71 +1.6 497,597 + nrdD MCAODC_02410 0.80 -0.1 497,597 + nrdD MCAODC_02410 0.80 +0.6 497,597 + nrdD MCAODC_02410 0.80 -1.4 497,598 - nrdD MCAODC_02410 0.80 -1.4 497,598 - nrdD MCAODC_02410 0.80 -0.3 497,598 - nrdD MCAODC_02410 0.80 -1.3 497,600 + nrdD MCAODC_02410 0.80 +0.5 497,600 + nrdD MCAODC_02410 0.80 -1.5 497,600 + nrdD MCAODC_02410 0.80 +0.5 497,600 + nrdD MCAODC_02410 0.80 +0.6 497,600 + nrdD MCAODC_02410 0.80 -0.7 497,600 + nrdD MCAODC_02410 0.80 -0.2 497,600 + nrdD MCAODC_02410 0.80 +0.2 497,601 - nrdD MCAODC_02410 0.80 -0.2 497,673 - nrdD MCAODC_02410 0.84 -1.1 497,715 - nrdD MCAODC_02410 0.86 +0.5 497,762 + nrdD MCAODC_02410 0.88 -0.1 497,762 + nrdD MCAODC_02410 0.88 +1.1 497,763 - nrdD MCAODC_02410 0.88 -0.2 497,768 + nrdD MCAODC_02410 0.88 +0.3 497,778 + nrdD MCAODC_02410 0.89 +1.8 497,778 + nrdD MCAODC_02410 0.89 -0.1 497,778 + nrdD MCAODC_02410 0.89 +2.0 497,779 - nrdD MCAODC_02410 0.89 +0.1 497,779 - nrdD MCAODC_02410 0.89 -1.9 497,783 + nrdD MCAODC_02410 0.89 +1.8 497,784 - nrdD MCAODC_02410 0.89 -0.4 497,784 - nrdD MCAODC_02410 0.89 -0.7 497,797 + nrdD MCAODC_02410 0.89 +0.7 497,798 - nrdD MCAODC_02410 0.89 +1.3 497,798 - nrdD MCAODC_02410 0.89 +0.2 497,798 - nrdD MCAODC_02410 0.89 +1.0 497,798 - nrdD MCAODC_02410 0.89 +1.9 497,816 + -0.2 497,817 - +0.8 497,817 - -0.1 497,907 + -0.5 497,907 + +0.1 497,931 + +0.0 497,931 + -0.0 497,931 + -0.8 497,931 + +0.4 497,931 + +0.9 497,932 - -0.4 497,932 - +0.1 497,932 - +0.2 497,932 - +0.5 497,932 - -0.5 497,956 + +0.2 497,981 - +0.6 497,981 - +0.9 498,015 + +0.3 498,016 - -1.5 498,016 - -0.9 498,030 + -0.9 498,030 + +0.4 498,030 + +0.5 498,128 - -1.5 498,183 - -0.7 498,211 - +0.3 498,211 - +0.2 498,211 - +0.3 498,218 + -0.6 498,218 + +1.9 498,219 - -0.2 498,219 - -0.0 498,227 + -0.4 498,227 + +1.2 498,227 + +1.5 498,227 + +0.6 498,227 + -0.0 498,228 - -1.9 498,228 - +1.6 498,228 - +0.1 498,228 - -2.0 498,228 - +2.7 498,230 + -0.4 498,230 + -1.5 498,231 - -0.2 498,231 - +1.2 498,311 + nrdG MCAODC_02420 0.21 +0.0 498,311 + nrdG MCAODC_02420 0.21 +1.7 498,311 + nrdG MCAODC_02420 0.21 -0.2 498,311 + nrdG MCAODC_02420 0.21 -0.3 498,312 - nrdG MCAODC_02420 0.22 +0.5 498,312 - nrdG MCAODC_02420 0.22 +0.4 498,312 - nrdG MCAODC_02420 0.22 -0.9 498,312 - nrdG MCAODC_02420 0.22 -0.3 498,312 - nrdG MCAODC_02420 0.22 +0.1 498,313 + nrdG MCAODC_02420 0.22 +0.2 498,313 + nrdG MCAODC_02420 0.22 +1.7 498,352 + nrdG MCAODC_02420 0.30 +0.5 498,353 - nrdG MCAODC_02420 0.30 -0.5 498,356 - nrdG MCAODC_02420 0.31 +0.0 498,401 - nrdG MCAODC_02420 0.41 +0.9 498,416 - nrdG MCAODC_02420 0.44 -0.4 498,477 + nrdG MCAODC_02420 0.57 -2.1 498,530 + nrdG MCAODC_02420 0.68 +1.7 498,677 + -3.2 498,677 + +1.7 498,677 + +0.4 498,677 + +0.5 498,677 + -1.3 498,678 - +0.2 498,678 - -0.9 498,678 - +0.5 498,722 + +0.1 498,722 + +0.0 498,729 - +2.0 498,729 - -0.1 498,729 - -0.1 498,790 + cybC MCAODC_02425 0.18 -0.8 498,791 - cybC MCAODC_02425 0.18 +0.3 498,796 - cybC MCAODC_02425 0.19 +0.5 498,891 + cybC MCAODC_02425 0.44 -0.1 498,892 - cybC MCAODC_02425 0.44 -0.7 499,000 + cybC MCAODC_02425 0.72 -0.4
Or see this region's nucleotide sequence