Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pepA and holC are separated by 353 nucleotides holC and valS overlap by 1 nucleotides valS and yjgN are separated by 54 nucleotides
MCAODC_02295: pepA - leucyl aminopeptidase, at 472,576 to 474,087
pepA
MCAODC_02315: holC - DNA polymerase III subunit chi, at 474,441 to 474,884
holC
MCAODC_02320: valS - valine--tRNA ligase, at 474,884 to 477,739
valS
MCAODC_02325: yjgN - Inner membrane protein YjgN, at 477,794 to 478,990
yjgN
Position (kb) 474
475
476
477
478 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 473.946 kb on + strand at 473.952 kb on + strand at 473.953 kb on - strand at 473.956 kb on + strand at 473.956 kb on + strand at 473.957 kb on - strand at 473.957 kb on - strand at 473.957 kb on - strand at 473.977 kb on - strand at 474.085 kb on + strand at 474.085 kb on + strand at 474.085 kb on + strand at 474.086 kb on - strand at 474.142 kb on + strand at 474.161 kb on - strand at 474.204 kb on - strand at 474.239 kb on - strand at 474.239 kb on - strand at 474.299 kb on + strand at 474.334 kb on + strand at 474.334 kb on + strand at 474.334 kb on + strand at 474.335 kb on - strand at 474.335 kb on - strand at 474.363 kb on + strand at 474.364 kb on - strand at 474.398 kb on + strand at 474.398 kb on + strand at 474.398 kb on + strand at 474.399 kb on - strand at 474.399 kb on - strand at 474.400 kb on + strand at 474.401 kb on - strand at 474.411 kb on + strand at 474.411 kb on + strand at 474.412 kb on - strand at 474.415 kb on + strand at 474.415 kb on + strand at 474.416 kb on - strand at 474.416 kb on - strand at 474.416 kb on - strand at 474.416 kb on - strand at 474.438 kb on + strand at 474.438 kb on + strand at 474.438 kb on + strand at 474.438 kb on + strand at 474.438 kb on + strand at 474.439 kb on - strand at 474.439 kb on - strand at 474.439 kb on - strand at 474.439 kb on - strand at 474.439 kb on - strand at 474.439 kb on - strand at 474.440 kb on + strand at 474.440 kb on + strand at 474.440 kb on + strand at 474.440 kb on + strand at 474.440 kb on + strand at 474.440 kb on + strand at 474.440 kb on + strand at 474.440 kb on + strand at 474.441 kb on - strand at 474.459 kb on + strand at 474.459 kb on + strand at 474.459 kb on + strand at 474.460 kb on - strand at 474.460 kb on - strand at 474.588 kb on + strand, within holC at 474.647 kb on - strand, within holC at 474.722 kb on + strand, within holC at 474.723 kb on - strand, within holC at 474.725 kb on - strand, within holC at 474.831 kb on + strand, within holC at 477.737 kb on + strand at 477.851 kb on - strand at 477.907 kb on - strand at 477.938 kb on + strand, within yjgN at 477.939 kb on - strand, within yjgN at 478.012 kb on + strand, within yjgN at 478.013 kb on - strand, within yjgN at 478.021 kb on - strand, within yjgN at 478.385 kb on + strand, within yjgN at 478.398 kb on - strand, within yjgN at 478.457 kb on + strand, within yjgN at 478.462 kb on + strand, within yjgN at 478.487 kb on + strand, within yjgN at 478.488 kb on - strand, within yjgN at 478.505 kb on - strand, within yjgN at 478.737 kb on + strand, within yjgN
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 473,946 + +0.3 473,952 + -2.0 473,953 - +0.4 473,956 + -1.1 473,956 + +0.7 473,957 - +0.5 473,957 - +0.2 473,957 - +0.6 473,977 - +0.1 474,085 + +1.0 474,085 + +0.7 474,085 + +0.7 474,086 - -0.6 474,142 + +0.1 474,161 - +0.8 474,204 - -2.5 474,239 - +0.9 474,239 - -0.0 474,299 + -0.1 474,334 + +0.2 474,334 + +0.6 474,334 + -0.4 474,335 - +0.2 474,335 - -1.1 474,363 + +1.2 474,364 - +0.1 474,398 + +0.1 474,398 + +1.0 474,398 + +1.3 474,399 - -1.5 474,399 - -0.7 474,400 + +1.0 474,401 - +1.2 474,411 + +1.3 474,411 + +0.4 474,412 - +0.4 474,415 + +1.3 474,415 + -1.4 474,416 - +0.1 474,416 - -0.2 474,416 - -0.3 474,416 - -0.8 474,438 + +0.2 474,438 + +0.0 474,438 + -1.1 474,438 + -0.0 474,438 + +0.5 474,439 - -0.6 474,439 - +0.9 474,439 - +1.0 474,439 - +1.8 474,439 - -0.7 474,439 - -0.5 474,440 + +0.6 474,440 + -0.0 474,440 + -0.8 474,440 + +0.1 474,440 + +0.3 474,440 + +0.5 474,440 + +0.2 474,440 + +0.4 474,441 - -0.1 474,459 + +1.2 474,459 + +0.3 474,459 + -1.6 474,460 - +0.9 474,460 - -1.1 474,588 + holC MCAODC_02315 0.33 -0.4 474,647 - holC MCAODC_02315 0.46 -0.6 474,722 + holC MCAODC_02315 0.63 -0.0 474,723 - holC MCAODC_02315 0.64 -3.4 474,725 - holC MCAODC_02315 0.64 -0.4 474,831 + holC MCAODC_02315 0.88 -1.9 477,737 + -0.3 477,851 - -0.2 477,907 - +1.3 477,938 + yjgN MCAODC_02325 0.12 +0.2 477,939 - yjgN MCAODC_02325 0.12 +0.5 478,012 + yjgN MCAODC_02325 0.18 -0.2 478,013 - yjgN MCAODC_02325 0.18 -1.1 478,021 - yjgN MCAODC_02325 0.19 -0.7 478,385 + yjgN MCAODC_02325 0.49 -0.5 478,398 - yjgN MCAODC_02325 0.50 -0.8 478,457 + yjgN MCAODC_02325 0.55 +2.6 478,462 + yjgN MCAODC_02325 0.56 -1.7 478,487 + yjgN MCAODC_02325 0.58 +0.9 478,488 - yjgN MCAODC_02325 0.58 -0.3 478,505 - yjgN MCAODC_02325 0.59 +1.7 478,737 + yjgN MCAODC_02325 0.79 -0.3
Or see this region's nucleotide sequence