Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yaaJ and yaaA are separated by 69 nucleotides yaaA and yaaX are separated by 152 nucleotides yaaX and thrC are separated by 213 nucleotides
MCAODC_01600: yaaJ - Uncharacterized transporter YaaJ, at 309,214 to 310,644
yaaJ
MCAODC_01605: yaaA - peroxide stress protein YaaA, at 310,714 to 311,490
yaaA
MCAODC_01610: yaaX - Uncharacterized protein YaaX, at 311,643 to 311,939
yaaX
MCAODC_01615: thrC - threonine synthase, at 312,153 to 313,439
thrC
Position (kb) 310
311
312 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 309.762 kb on + strand, within yaaJ at 309.851 kb on + strand, within yaaJ at 309.860 kb on + strand, within yaaJ at 309.888 kb on - strand, within yaaJ at 309.975 kb on + strand, within yaaJ at 309.975 kb on + strand, within yaaJ at 309.975 kb on + strand, within yaaJ at 309.975 kb on + strand, within yaaJ at 309.976 kb on - strand, within yaaJ at 309.976 kb on - strand, within yaaJ at 309.976 kb on - strand, within yaaJ at 309.981 kb on - strand, within yaaJ at 309.996 kb on + strand, within yaaJ at 310.014 kb on + strand, within yaaJ at 310.014 kb on + strand, within yaaJ at 310.014 kb on + strand, within yaaJ at 310.166 kb on + strand, within yaaJ at 310.167 kb on - strand, within yaaJ at 310.167 kb on - strand, within yaaJ at 310.169 kb on + strand, within yaaJ at 310.170 kb on - strand, within yaaJ at 310.192 kb on + strand, within yaaJ at 310.192 kb on + strand, within yaaJ at 310.192 kb on + strand, within yaaJ at 310.192 kb on + strand, within yaaJ at 310.192 kb on + strand, within yaaJ at 310.192 kb on + strand, within yaaJ at 310.192 kb on + strand, within yaaJ at 310.192 kb on + strand, within yaaJ at 310.192 kb on + strand, within yaaJ at 310.192 kb on + strand, within yaaJ at 310.193 kb on - strand, within yaaJ at 310.193 kb on - strand, within yaaJ at 310.193 kb on - strand, within yaaJ at 310.193 kb on - strand, within yaaJ at 310.218 kb on + strand, within yaaJ at 310.242 kb on - strand, within yaaJ at 310.315 kb on + strand, within yaaJ at 310.362 kb on + strand, within yaaJ at 310.422 kb on - strand, within yaaJ at 310.422 kb on - strand, within yaaJ at 310.422 kb on - strand, within yaaJ at 310.422 kb on - strand, within yaaJ at 310.453 kb on - strand, within yaaJ at 310.457 kb on + strand, within yaaJ at 310.457 kb on + strand, within yaaJ at 310.457 kb on + strand, within yaaJ at 310.473 kb on + strand, within yaaJ at 310.473 kb on + strand, within yaaJ at 310.474 kb on - strand, within yaaJ at 310.474 kb on - strand, within yaaJ at 310.476 kb on + strand, within yaaJ at 310.493 kb on + strand, within yaaJ at 310.494 kb on - strand, within yaaJ at 310.494 kb on - strand, within yaaJ at 310.494 kb on - strand, within yaaJ at 310.494 kb on - strand, within yaaJ at 310.516 kb on + strand at 310.516 kb on + strand at 310.516 kb on + strand at 310.517 kb on - strand at 310.517 kb on - strand at 310.518 kb on + strand at 310.519 kb on - strand at 310.535 kb on - strand at 310.535 kb on - strand at 310.538 kb on + strand at 310.538 kb on + strand at 310.539 kb on - strand at 310.549 kb on - strand at 310.642 kb on + strand at 310.652 kb on + strand at 310.652 kb on + strand at 310.653 kb on - strand at 310.653 kb on - strand at 310.681 kb on - strand at 310.725 kb on + strand at 310.779 kb on + strand at 310.779 kb on + strand at 310.779 kb on + strand at 310.779 kb on + strand at 310.779 kb on + strand at 310.779 kb on + strand at 310.779 kb on + strand at 310.779 kb on + strand at 310.780 kb on - strand at 310.780 kb on - strand at 310.780 kb on - strand at 310.796 kb on + strand, within yaaA at 310.866 kb on + strand, within yaaA at 310.867 kb on - strand, within yaaA at 310.887 kb on + strand, within yaaA at 310.887 kb on + strand, within yaaA at 310.975 kb on + strand, within yaaA at 310.975 kb on + strand, within yaaA at 310.976 kb on - strand, within yaaA at 310.976 kb on - strand, within yaaA at 311.057 kb on - strand, within yaaA at 311.063 kb on + strand, within yaaA at 311.063 kb on + strand, within yaaA at 311.063 kb on + strand, within yaaA at 311.081 kb on + strand, within yaaA at 311.081 kb on + strand, within yaaA at 311.082 kb on - strand, within yaaA at 311.082 kb on - strand, within yaaA at 311.144 kb on - strand, within yaaA at 311.160 kb on + strand, within yaaA at 311.236 kb on + strand, within yaaA at 311.236 kb on + strand, within yaaA at 311.236 kb on + strand, within yaaA at 311.242 kb on - strand, within yaaA at 311.321 kb on + strand, within yaaA at 311.321 kb on + strand, within yaaA at 311.321 kb on + strand, within yaaA at 311.332 kb on + strand, within yaaA at 311.332 kb on + strand, within yaaA at 311.332 kb on + strand, within yaaA at 311.332 kb on + strand, within yaaA at 311.333 kb on - strand, within yaaA at 311.412 kb on + strand, within yaaA at 311.412 kb on + strand, within yaaA at 311.425 kb on + strand at 311.426 kb on - strand at 311.496 kb on + strand at 311.497 kb on - strand at 311.497 kb on - strand at 311.556 kb on + strand at 311.557 kb on - strand at 311.557 kb on - strand at 311.557 kb on - strand at 311.558 kb on + strand at 311.699 kb on + strand, within yaaX at 311.699 kb on + strand, within yaaX at 311.758 kb on + strand, within yaaX at 311.758 kb on + strand, within yaaX at 311.758 kb on + strand, within yaaX at 311.759 kb on - strand, within yaaX at 311.759 kb on - strand, within yaaX at 311.806 kb on + strand, within yaaX at 311.810 kb on - strand, within yaaX at 311.810 kb on - strand, within yaaX at 311.839 kb on - strand, within yaaX at 311.839 kb on - strand, within yaaX at 311.861 kb on + strand, within yaaX at 311.861 kb on + strand, within yaaX at 311.862 kb on - strand, within yaaX at 311.921 kb on + strand at 311.921 kb on + strand at 311.922 kb on - strand at 311.953 kb on + strand at 311.954 kb on - strand at 311.954 kb on - strand at 311.954 kb on - strand at 311.954 kb on - strand at 312.114 kb on + strand at 312.115 kb on - strand at 312.147 kb on + strand at 312.148 kb on - strand at 312.148 kb on - strand at 312.148 kb on - strand at 312.154 kb on + strand at 312.154 kb on + strand at 312.155 kb on - strand at 312.155 kb on - strand at 312.206 kb on + strand at 312.222 kb on + strand at 312.294 kb on - strand, within thrC at 312.397 kb on + strand, within thrC at 312.457 kb on + strand, within thrC
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 309,762 + yaaJ MCAODC_01600 0.38 -0.9 309,851 + yaaJ MCAODC_01600 0.45 -2.4 309,860 + yaaJ MCAODC_01600 0.45 -0.4 309,888 - yaaJ MCAODC_01600 0.47 -0.4 309,975 + yaaJ MCAODC_01600 0.53 -0.5 309,975 + yaaJ MCAODC_01600 0.53 +1.3 309,975 + yaaJ MCAODC_01600 0.53 +0.8 309,975 + yaaJ MCAODC_01600 0.53 +1.2 309,976 - yaaJ MCAODC_01600 0.53 -0.1 309,976 - yaaJ MCAODC_01600 0.53 -0.3 309,976 - yaaJ MCAODC_01600 0.53 -0.7 309,981 - yaaJ MCAODC_01600 0.54 +0.5 309,996 + yaaJ MCAODC_01600 0.55 -0.1 310,014 + yaaJ MCAODC_01600 0.56 +0.4 310,014 + yaaJ MCAODC_01600 0.56 -1.6 310,014 + yaaJ MCAODC_01600 0.56 -0.4 310,166 + yaaJ MCAODC_01600 0.67 -0.2 310,167 - yaaJ MCAODC_01600 0.67 -0.2 310,167 - yaaJ MCAODC_01600 0.67 -1.0 310,169 + yaaJ MCAODC_01600 0.67 +0.4 310,170 - yaaJ MCAODC_01600 0.67 -0.2 310,192 + yaaJ MCAODC_01600 0.68 +0.1 310,192 + yaaJ MCAODC_01600 0.68 +1.4 310,192 + yaaJ MCAODC_01600 0.68 +0.0 310,192 + yaaJ MCAODC_01600 0.68 -0.4 310,192 + yaaJ MCAODC_01600 0.68 -3.3 310,192 + yaaJ MCAODC_01600 0.68 +0.8 310,192 + yaaJ MCAODC_01600 0.68 -1.1 310,192 + yaaJ MCAODC_01600 0.68 +1.0 310,192 + yaaJ MCAODC_01600 0.68 -0.6 310,192 + yaaJ MCAODC_01600 0.68 -1.3 310,193 - yaaJ MCAODC_01600 0.68 -0.5 310,193 - yaaJ MCAODC_01600 0.68 -2.1 310,193 - yaaJ MCAODC_01600 0.68 +0.6 310,193 - yaaJ MCAODC_01600 0.68 +0.1 310,218 + yaaJ MCAODC_01600 0.70 +0.4 310,242 - yaaJ MCAODC_01600 0.72 +0.1 310,315 + yaaJ MCAODC_01600 0.77 +0.3 310,362 + yaaJ MCAODC_01600 0.80 +0.6 310,422 - yaaJ MCAODC_01600 0.84 -1.6 310,422 - yaaJ MCAODC_01600 0.84 -0.0 310,422 - yaaJ MCAODC_01600 0.84 -3.4 310,422 - yaaJ MCAODC_01600 0.84 +1.2 310,453 - yaaJ MCAODC_01600 0.87 -2.1 310,457 + yaaJ MCAODC_01600 0.87 -1.1 310,457 + yaaJ MCAODC_01600 0.87 -0.2 310,457 + yaaJ MCAODC_01600 0.87 +1.6 310,473 + yaaJ MCAODC_01600 0.88 +0.2 310,473 + yaaJ MCAODC_01600 0.88 +0.4 310,474 - yaaJ MCAODC_01600 0.88 +0.3 310,474 - yaaJ MCAODC_01600 0.88 -0.2 310,476 + yaaJ MCAODC_01600 0.88 -0.8 310,493 + yaaJ MCAODC_01600 0.89 -2.4 310,494 - yaaJ MCAODC_01600 0.89 +0.2 310,494 - yaaJ MCAODC_01600 0.89 +2.0 310,494 - yaaJ MCAODC_01600 0.89 -2.4 310,494 - yaaJ MCAODC_01600 0.89 +0.3 310,516 + +0.4 310,516 + -0.5 310,516 + +0.1 310,517 - -2.8 310,517 - -0.3 310,518 + +1.0 310,519 - +0.8 310,535 - +0.3 310,535 - -1.7 310,538 + -1.3 310,538 + -3.4 310,539 - +0.1 310,549 - -1.2 310,642 + -0.5 310,652 + +1.6 310,652 + -3.0 310,653 - +1.2 310,653 - +0.1 310,681 - +1.4 310,725 + -1.9 310,779 + -2.7 310,779 + +0.3 310,779 + +0.4 310,779 + -0.2 310,779 + +0.2 310,779 + +0.6 310,779 + -0.4 310,779 + -1.0 310,780 - +0.0 310,780 - +2.0 310,780 - -0.9 310,796 + yaaA MCAODC_01605 0.11 -0.2 310,866 + yaaA MCAODC_01605 0.20 -1.2 310,867 - yaaA MCAODC_01605 0.20 -0.5 310,887 + yaaA MCAODC_01605 0.22 +0.1 310,887 + yaaA MCAODC_01605 0.22 +0.6 310,975 + yaaA MCAODC_01605 0.34 +0.1 310,975 + yaaA MCAODC_01605 0.34 -0.0 310,976 - yaaA MCAODC_01605 0.34 +0.1 310,976 - yaaA MCAODC_01605 0.34 -0.5 311,057 - yaaA MCAODC_01605 0.44 +1.8 311,063 + yaaA MCAODC_01605 0.45 +0.4 311,063 + yaaA MCAODC_01605 0.45 +0.9 311,063 + yaaA MCAODC_01605 0.45 +1.0 311,081 + yaaA MCAODC_01605 0.47 +2.6 311,081 + yaaA MCAODC_01605 0.47 +0.0 311,082 - yaaA MCAODC_01605 0.47 +0.9 311,082 - yaaA MCAODC_01605 0.47 +0.9 311,144 - yaaA MCAODC_01605 0.55 +0.1 311,160 + yaaA MCAODC_01605 0.57 -1.2 311,236 + yaaA MCAODC_01605 0.67 -0.5 311,236 + yaaA MCAODC_01605 0.67 -0.6 311,236 + yaaA MCAODC_01605 0.67 +1.0 311,242 - yaaA MCAODC_01605 0.68 +0.7 311,321 + yaaA MCAODC_01605 0.78 -0.7 311,321 + yaaA MCAODC_01605 0.78 +1.4 311,321 + yaaA MCAODC_01605 0.78 -0.9 311,332 + yaaA MCAODC_01605 0.80 -0.2 311,332 + yaaA MCAODC_01605 0.80 +0.5 311,332 + yaaA MCAODC_01605 0.80 -0.2 311,332 + yaaA MCAODC_01605 0.80 -2.1 311,333 - yaaA MCAODC_01605 0.80 +0.1 311,412 + yaaA MCAODC_01605 0.90 +0.4 311,412 + yaaA MCAODC_01605 0.90 -0.2 311,425 + +0.9 311,426 - -0.5 311,496 + -1.1 311,497 - -0.1 311,497 - +0.2 311,556 + +0.1 311,557 - +1.3 311,557 - +0.9 311,557 - +0.1 311,558 + +0.3 311,699 + yaaX MCAODC_01610 0.19 -0.3 311,699 + yaaX MCAODC_01610 0.19 +1.7 311,758 + yaaX MCAODC_01610 0.39 +0.5 311,758 + yaaX MCAODC_01610 0.39 +0.1 311,758 + yaaX MCAODC_01610 0.39 -0.8 311,759 - yaaX MCAODC_01610 0.39 -1.8 311,759 - yaaX MCAODC_01610 0.39 +0.2 311,806 + yaaX MCAODC_01610 0.55 -0.8 311,810 - yaaX MCAODC_01610 0.56 -1.6 311,810 - yaaX MCAODC_01610 0.56 +0.1 311,839 - yaaX MCAODC_01610 0.66 +1.2 311,839 - yaaX MCAODC_01610 0.66 +0.2 311,861 + yaaX MCAODC_01610 0.73 -0.6 311,861 + yaaX MCAODC_01610 0.73 +0.3 311,862 - yaaX MCAODC_01610 0.74 +0.6 311,921 + +0.0 311,921 + +0.7 311,922 - -0.2 311,953 + +0.2 311,954 - -0.4 311,954 - -0.1 311,954 - -0.1 311,954 - -1.5 312,114 + +0.2 312,115 - +1.1 312,147 + +2.0 312,148 - -1.0 312,148 - -0.2 312,148 - -0.1 312,154 + +1.7 312,154 + +1.5 312,155 - +0.7 312,155 - +1.6 312,206 + +0.1 312,222 + +0.4 312,294 - thrC MCAODC_01615 0.11 +0.5 312,397 + thrC MCAODC_01615 0.19 +1.1 312,457 + thrC MCAODC_01615 0.24 -0.1
Or see this region's nucleotide sequence