Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fimD and fimA are separated by 13 nucleotides fimA and fimA are separated by 14 nucleotides fimA and fimA are separated by 20 nucleotides fimA and fimA are separated by 11 nucleotides
MCAODC_00890: fimD - outer membrane usher protein, at 157,369 to 159,969
fimD
MCAODC_00895: fimA - Pilin (type 1 fimbrial protein), at 159,983 to 160,531
fimA
MCAODC_00900: fimA - fimbrial protein, at 160,546 to 161,151
fimA
MCAODC_00905: fimA - fimbrial-like protein, at 161,172 to 161,762
fimA
MCAODC_00910: fimA - fimbrial protein, at 161,774 to 162,883
fimA
Position (kb) 160
161
162 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 159.625 kb on - strand, within fimD at 159.641 kb on + strand, within fimD at 159.641 kb on + strand, within fimD at 159.642 kb on - strand, within fimD at 159.648 kb on + strand, within fimD at 159.708 kb on + strand, within fimD at 159.712 kb on - strand at 159.833 kb on + strand at 159.847 kb on + strand at 159.873 kb on + strand at 159.873 kb on + strand at 159.873 kb on + strand at 159.874 kb on - strand at 159.874 kb on - strand at 159.874 kb on - strand at 159.874 kb on - strand at 159.883 kb on + strand at 159.929 kb on + strand at 159.929 kb on + strand at 159.929 kb on + strand at 159.932 kb on + strand at 159.932 kb on + strand at 159.932 kb on + strand at 159.932 kb on + strand at 159.932 kb on + strand at 159.932 kb on + strand at 159.933 kb on - strand at 159.933 kb on - strand at 159.933 kb on - strand at 159.936 kb on + strand at 159.937 kb on - strand at 159.945 kb on + strand at 159.945 kb on + strand at 159.967 kb on + strand at 159.967 kb on + strand at 159.968 kb on - strand at 159.993 kb on + strand at 159.996 kb on + strand at 159.996 kb on + strand at 159.997 kb on - strand at 159.997 kb on - strand at 159.997 kb on - strand at 159.997 kb on - strand at 159.997 kb on - strand at 159.999 kb on + strand at 159.999 kb on + strand at 160.000 kb on - strand at 160.015 kb on - strand at 160.055 kb on - strand, within fimA at 160.055 kb on - strand, within fimA at 160.055 kb on - strand, within fimA at 160.066 kb on + strand, within fimA at 160.099 kb on - strand, within fimA at 160.099 kb on - strand, within fimA at 160.102 kb on + strand, within fimA at 160.102 kb on + strand, within fimA at 160.102 kb on + strand, within fimA at 160.102 kb on + strand, within fimA at 160.102 kb on + strand, within fimA at 160.102 kb on + strand, within fimA at 160.102 kb on + strand, within fimA at 160.102 kb on + strand, within fimA at 160.103 kb on - strand, within fimA at 160.103 kb on - strand, within fimA at 160.103 kb on - strand, within fimA at 160.103 kb on - strand, within fimA at 160.103 kb on - strand, within fimA at 160.103 kb on - strand, within fimA at 160.103 kb on - strand, within fimA at 160.103 kb on - strand, within fimA at 160.103 kb on - strand, within fimA at 160.105 kb on - strand, within fimA at 160.148 kb on + strand, within fimA at 160.149 kb on - strand, within fimA at 160.220 kb on - strand, within fimA at 160.273 kb on + strand, within fimA at 160.273 kb on + strand, within fimA at 160.274 kb on - strand, within fimA at 160.274 kb on - strand, within fimA at 160.294 kb on + strand, within fimA at 160.299 kb on + strand, within fimA at 160.348 kb on + strand, within fimA at 160.354 kb on + strand, within fimA at 160.360 kb on + strand, within fimA at 160.360 kb on + strand, within fimA at 160.393 kb on + strand, within fimA at 160.394 kb on - strand, within fimA at 160.514 kb on - strand at 160.554 kb on - strand at 160.578 kb on + strand at 160.578 kb on + strand at 160.595 kb on + strand at 160.596 kb on - strand at 160.596 kb on - strand at 160.627 kb on - strand, within fimA at 160.627 kb on - strand, within fimA at 160.650 kb on - strand, within fimA at 160.656 kb on + strand, within fimA at 160.672 kb on - strand, within fimA at 160.672 kb on - strand, within fimA at 160.672 kb on - strand, within fimA at 160.698 kb on + strand, within fimA at 160.698 kb on + strand, within fimA at 160.698 kb on + strand, within fimA at 160.698 kb on + strand, within fimA at 160.699 kb on - strand, within fimA at 160.699 kb on - strand, within fimA at 160.702 kb on - strand, within fimA at 160.705 kb on - strand, within fimA at 160.705 kb on - strand, within fimA at 160.739 kb on - strand, within fimA at 160.740 kb on + strand, within fimA at 160.741 kb on - strand, within fimA at 160.791 kb on + strand, within fimA at 160.791 kb on + strand, within fimA at 160.829 kb on + strand, within fimA at 160.829 kb on + strand, within fimA at 160.830 kb on - strand, within fimA at 160.842 kb on + strand, within fimA at 160.843 kb on - strand, within fimA at 160.843 kb on - strand, within fimA at 160.843 kb on - strand, within fimA at 160.843 kb on - strand, within fimA at 160.843 kb on - strand, within fimA at 160.843 kb on - strand, within fimA at 160.843 kb on - strand, within fimA at 160.848 kb on + strand, within fimA at 160.848 kb on + strand, within fimA at 160.848 kb on + strand, within fimA at 160.848 kb on + strand, within fimA at 160.848 kb on + strand, within fimA at 160.848 kb on + strand, within fimA at 160.848 kb on + strand, within fimA at 160.849 kb on - strand, within fimA at 160.849 kb on - strand, within fimA at 160.849 kb on - strand, within fimA at 160.849 kb on - strand, within fimA at 160.849 kb on - strand, within fimA at 160.849 kb on - strand, within fimA at 160.849 kb on - strand, within fimA at 160.850 kb on + strand, within fimA at 160.851 kb on - strand, within fimA at 160.851 kb on - strand, within fimA at 160.909 kb on - strand, within fimA at 160.937 kb on + strand, within fimA at 160.938 kb on - strand, within fimA at 160.938 kb on - strand, within fimA at 160.944 kb on + strand, within fimA at 160.944 kb on + strand, within fimA at 160.945 kb on - strand, within fimA at 160.950 kb on + strand, within fimA at 160.951 kb on - strand, within fimA at 160.951 kb on - strand, within fimA at 160.997 kb on + strand, within fimA at 161.041 kb on + strand, within fimA at 161.042 kb on - strand, within fimA at 161.042 kb on - strand, within fimA at 161.055 kb on - strand, within fimA at 161.083 kb on - strand, within fimA at 161.083 kb on - strand, within fimA at 161.083 kb on - strand, within fimA at 161.083 kb on - strand, within fimA at 161.099 kb on + strand at 161.100 kb on - strand at 161.143 kb on + strand at 161.143 kb on + strand at 161.144 kb on - strand at 161.149 kb on + strand at 161.149 kb on + strand at 161.150 kb on - strand at 161.150 kb on - strand at 161.153 kb on - strand at 161.168 kb on + strand at 161.169 kb on - strand at 161.169 kb on - strand at 161.169 kb on - strand at 161.169 kb on - strand at 161.176 kb on + strand at 161.176 kb on + strand at 161.177 kb on - strand at 161.177 kb on - strand at 161.190 kb on - strand at 161.190 kb on - strand at 161.197 kb on + strand at 161.241 kb on + strand, within fimA at 161.306 kb on + strand, within fimA at 161.306 kb on + strand, within fimA at 161.347 kb on - strand, within fimA at 161.347 kb on - strand, within fimA at 161.362 kb on + strand, within fimA at 161.362 kb on + strand, within fimA at 161.363 kb on - strand, within fimA at 161.379 kb on + strand, within fimA at 161.518 kb on - strand, within fimA at 161.518 kb on - strand, within fimA at 161.591 kb on + strand, within fimA at 161.673 kb on + strand, within fimA at 161.674 kb on - strand, within fimA at 161.674 kb on - strand, within fimA at 161.749 kb on + strand at 161.749 kb on + strand at 161.750 kb on - strand at 161.750 kb on - strand at 161.750 kb on - strand at 161.750 kb on - strand at 161.750 kb on - strand at 161.769 kb on + strand at 161.770 kb on - strand at 161.773 kb on + strand at 161.774 kb on - strand at 161.820 kb on + strand at 161.846 kb on + strand at 161.867 kb on + strand at 161.871 kb on + strand at 161.872 kb on - strand at 161.908 kb on - strand, within fimA at 161.908 kb on - strand, within fimA at 161.908 kb on - strand, within fimA at 161.914 kb on + strand, within fimA at 161.914 kb on + strand, within fimA at 161.923 kb on + strand, within fimA at 161.961 kb on + strand, within fimA at 161.962 kb on - strand, within fimA at 161.971 kb on + strand, within fimA at 161.982 kb on + strand, within fimA at 161.983 kb on - strand, within fimA at 161.983 kb on - strand, within fimA at 161.994 kb on + strand, within fimA at 161.994 kb on + strand, within fimA at 162.009 kb on + strand, within fimA at 162.016 kb on + strand, within fimA at 162.031 kb on + strand, within fimA at 162.055 kb on + strand, within fimA at 162.072 kb on + strand, within fimA at 162.075 kb on + strand, within fimA at 162.083 kb on + strand, within fimA at 162.083 kb on + strand, within fimA at 162.083 kb on + strand, within fimA at 162.084 kb on - strand, within fimA at 162.084 kb on - strand, within fimA at 162.109 kb on - strand, within fimA at 162.137 kb on + strand, within fimA at 162.138 kb on - strand, within fimA at 162.138 kb on - strand, within fimA at 162.138 kb on - strand, within fimA at 162.151 kb on - strand, within fimA at 162.151 kb on - strand, within fimA at 162.151 kb on - strand, within fimA
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 159,625 - fimD MCAODC_00890 0.87 -1.0 159,641 + fimD MCAODC_00890 0.87 +0.9 159,641 + fimD MCAODC_00890 0.87 -0.3 159,642 - fimD MCAODC_00890 0.87 +1.2 159,648 + fimD MCAODC_00890 0.88 +0.0 159,708 + fimD MCAODC_00890 0.90 -0.5 159,712 - -0.4 159,833 + -1.0 159,847 + -0.3 159,873 + +2.2 159,873 + -0.1 159,873 + +0.6 159,874 - +0.3 159,874 - +0.6 159,874 - +1.1 159,874 - +1.3 159,883 + +0.9 159,929 + -0.4 159,929 + -3.0 159,929 + -0.4 159,932 + -0.6 159,932 + -0.3 159,932 + +1.3 159,932 + +0.5 159,932 + +0.7 159,932 + -0.0 159,933 - +1.6 159,933 - -0.5 159,933 - +0.9 159,936 + +0.9 159,937 - +0.4 159,945 + +0.2 159,945 + -1.3 159,967 + +0.7 159,967 + +1.2 159,968 - -0.8 159,993 + +0.1 159,996 + -0.1 159,996 + +0.0 159,997 - +1.2 159,997 - +0.9 159,997 - -0.1 159,997 - -0.2 159,997 - +1.0 159,999 + +0.7 159,999 + +0.8 160,000 - +1.2 160,015 - +1.2 160,055 - fimA MCAODC_00895 0.13 -1.2 160,055 - fimA MCAODC_00895 0.13 +0.3 160,055 - fimA MCAODC_00895 0.13 +0.4 160,066 + fimA MCAODC_00895 0.15 +1.3 160,099 - fimA MCAODC_00895 0.21 +1.5 160,099 - fimA MCAODC_00895 0.21 +0.4 160,102 + fimA MCAODC_00895 0.22 -0.0 160,102 + fimA MCAODC_00895 0.22 +0.7 160,102 + fimA MCAODC_00895 0.22 -0.1 160,102 + fimA MCAODC_00895 0.22 -0.4 160,102 + fimA MCAODC_00895 0.22 -1.9 160,102 + fimA MCAODC_00895 0.22 -0.1 160,102 + fimA MCAODC_00895 0.22 +0.2 160,102 + fimA MCAODC_00895 0.22 +0.4 160,103 - fimA MCAODC_00895 0.22 +0.7 160,103 - fimA MCAODC_00895 0.22 -2.3 160,103 - fimA MCAODC_00895 0.22 -0.5 160,103 - fimA MCAODC_00895 0.22 -0.1 160,103 - fimA MCAODC_00895 0.22 -0.8 160,103 - fimA MCAODC_00895 0.22 -1.2 160,103 - fimA MCAODC_00895 0.22 +0.7 160,103 - fimA MCAODC_00895 0.22 -0.9 160,103 - fimA MCAODC_00895 0.22 -0.4 160,105 - fimA MCAODC_00895 0.22 -0.0 160,148 + fimA MCAODC_00895 0.30 -0.4 160,149 - fimA MCAODC_00895 0.30 +0.4 160,220 - fimA MCAODC_00895 0.43 -0.3 160,273 + fimA MCAODC_00895 0.53 -0.4 160,273 + fimA MCAODC_00895 0.53 -0.8 160,274 - fimA MCAODC_00895 0.53 -0.7 160,274 - fimA MCAODC_00895 0.53 -0.1 160,294 + fimA MCAODC_00895 0.57 +1.0 160,299 + fimA MCAODC_00895 0.58 +0.8 160,348 + fimA MCAODC_00895 0.66 +0.2 160,354 + fimA MCAODC_00895 0.68 -1.5 160,360 + fimA MCAODC_00895 0.69 +0.3 160,360 + fimA MCAODC_00895 0.69 -0.2 160,393 + fimA MCAODC_00895 0.75 -0.7 160,394 - fimA MCAODC_00895 0.75 -0.7 160,514 - +1.0 160,554 - -0.2 160,578 + +0.1 160,578 + +0.4 160,595 + -0.6 160,596 - -1.9 160,596 - -0.8 160,627 - fimA MCAODC_00900 0.13 -0.2 160,627 - fimA MCAODC_00900 0.13 -1.4 160,650 - fimA MCAODC_00900 0.17 -0.8 160,656 + fimA MCAODC_00900 0.18 -0.3 160,672 - fimA MCAODC_00900 0.21 +0.5 160,672 - fimA MCAODC_00900 0.21 +0.5 160,672 - fimA MCAODC_00900 0.21 +1.6 160,698 + fimA MCAODC_00900 0.25 -0.6 160,698 + fimA MCAODC_00900 0.25 +0.6 160,698 + fimA MCAODC_00900 0.25 +1.3 160,698 + fimA MCAODC_00900 0.25 -1.2 160,699 - fimA MCAODC_00900 0.25 +0.7 160,699 - fimA MCAODC_00900 0.25 -0.2 160,702 - fimA MCAODC_00900 0.26 -0.1 160,705 - fimA MCAODC_00900 0.26 +1.0 160,705 - fimA MCAODC_00900 0.26 +0.6 160,739 - fimA MCAODC_00900 0.32 +0.1 160,740 + fimA MCAODC_00900 0.32 +0.8 160,741 - fimA MCAODC_00900 0.32 +0.1 160,791 + fimA MCAODC_00900 0.40 -0.3 160,791 + fimA MCAODC_00900 0.40 -0.3 160,829 + fimA MCAODC_00900 0.47 +1.1 160,829 + fimA MCAODC_00900 0.47 +0.4 160,830 - fimA MCAODC_00900 0.47 +1.1 160,842 + fimA MCAODC_00900 0.49 +0.4 160,843 - fimA MCAODC_00900 0.49 +0.8 160,843 - fimA MCAODC_00900 0.49 +0.1 160,843 - fimA MCAODC_00900 0.49 +0.2 160,843 - fimA MCAODC_00900 0.49 +0.1 160,843 - fimA MCAODC_00900 0.49 +0.1 160,843 - fimA MCAODC_00900 0.49 -2.4 160,843 - fimA MCAODC_00900 0.49 +0.9 160,848 + fimA MCAODC_00900 0.50 -0.0 160,848 + fimA MCAODC_00900 0.50 +0.9 160,848 + fimA MCAODC_00900 0.50 +2.9 160,848 + fimA MCAODC_00900 0.50 -0.6 160,848 + fimA MCAODC_00900 0.50 -0.1 160,848 + fimA MCAODC_00900 0.50 +0.7 160,848 + fimA MCAODC_00900 0.50 -1.4 160,849 - fimA MCAODC_00900 0.50 +0.6 160,849 - fimA MCAODC_00900 0.50 -0.1 160,849 - fimA MCAODC_00900 0.50 -1.6 160,849 - fimA MCAODC_00900 0.50 -0.0 160,849 - fimA MCAODC_00900 0.50 +0.4 160,849 - fimA MCAODC_00900 0.50 -0.1 160,849 - fimA MCAODC_00900 0.50 +0.1 160,850 + fimA MCAODC_00900 0.50 -0.1 160,851 - fimA MCAODC_00900 0.50 -0.1 160,851 - fimA MCAODC_00900 0.50 +0.0 160,909 - fimA MCAODC_00900 0.60 +1.3 160,937 + fimA MCAODC_00900 0.65 -1.2 160,938 - fimA MCAODC_00900 0.65 -0.4 160,938 - fimA MCAODC_00900 0.65 +0.1 160,944 + fimA MCAODC_00900 0.66 +1.5 160,944 + fimA MCAODC_00900 0.66 +0.7 160,945 - fimA MCAODC_00900 0.66 +0.2 160,950 + fimA MCAODC_00900 0.67 -0.3 160,951 - fimA MCAODC_00900 0.67 +1.2 160,951 - fimA MCAODC_00900 0.67 -3.6 160,997 + fimA MCAODC_00900 0.74 -3.5 161,041 + fimA MCAODC_00900 0.82 +0.9 161,042 - fimA MCAODC_00900 0.82 +1.7 161,042 - fimA MCAODC_00900 0.82 +0.7 161,055 - fimA MCAODC_00900 0.84 -0.0 161,083 - fimA MCAODC_00900 0.89 +0.4 161,083 - fimA MCAODC_00900 0.89 +0.5 161,083 - fimA MCAODC_00900 0.89 -0.1 161,083 - fimA MCAODC_00900 0.89 +0.5 161,099 + +0.4 161,100 - +0.5 161,143 + -0.1 161,143 + -2.5 161,144 - -1.1 161,149 + +0.2 161,149 + +2.0 161,150 - -0.3 161,150 - -0.4 161,153 - +0.1 161,168 + +0.6 161,169 - -0.7 161,169 - +0.6 161,169 - -0.1 161,169 - -0.1 161,176 + -0.2 161,176 + -0.7 161,177 - -0.1 161,177 - -2.3 161,190 - +0.4 161,190 - +1.0 161,197 + +0.1 161,241 + fimA MCAODC_00905 0.12 +0.2 161,306 + fimA MCAODC_00905 0.23 -1.1 161,306 + fimA MCAODC_00905 0.23 +0.4 161,347 - fimA MCAODC_00905 0.30 -0.4 161,347 - fimA MCAODC_00905 0.30 -1.1 161,362 + fimA MCAODC_00905 0.32 -0.6 161,362 + fimA MCAODC_00905 0.32 +0.2 161,363 - fimA MCAODC_00905 0.32 -1.7 161,379 + fimA MCAODC_00905 0.35 +0.1 161,518 - fimA MCAODC_00905 0.59 +0.3 161,518 - fimA MCAODC_00905 0.59 +0.4 161,591 + fimA MCAODC_00905 0.71 +2.9 161,673 + fimA MCAODC_00905 0.85 +0.2 161,674 - fimA MCAODC_00905 0.85 +0.8 161,674 - fimA MCAODC_00905 0.85 +0.8 161,749 + +1.2 161,749 + +0.7 161,750 - -0.3 161,750 - -0.7 161,750 - -0.1 161,750 - +0.5 161,750 - -0.7 161,769 + +1.0 161,770 - +1.3 161,773 + +0.2 161,774 - -0.2 161,820 + -0.9 161,846 + +0.4 161,867 + -0.2 161,871 + +1.4 161,872 - +0.6 161,908 - fimA MCAODC_00910 0.12 +0.6 161,908 - fimA MCAODC_00910 0.12 +1.1 161,908 - fimA MCAODC_00910 0.12 +0.1 161,914 + fimA MCAODC_00910 0.13 +0.1 161,914 + fimA MCAODC_00910 0.13 +0.2 161,923 + fimA MCAODC_00910 0.13 +0.2 161,961 + fimA MCAODC_00910 0.17 +2.0 161,962 - fimA MCAODC_00910 0.17 +0.5 161,971 + fimA MCAODC_00910 0.18 -0.5 161,982 + fimA MCAODC_00910 0.19 -1.6 161,983 - fimA MCAODC_00910 0.19 +0.3 161,983 - fimA MCAODC_00910 0.19 -0.8 161,994 + fimA MCAODC_00910 0.20 +0.5 161,994 + fimA MCAODC_00910 0.20 +0.1 162,009 + fimA MCAODC_00910 0.21 -0.3 162,016 + fimA MCAODC_00910 0.22 -0.1 162,031 + fimA MCAODC_00910 0.23 +0.1 162,055 + fimA MCAODC_00910 0.25 +0.9 162,072 + fimA MCAODC_00910 0.27 -2.4 162,075 + fimA MCAODC_00910 0.27 -0.4 162,083 + fimA MCAODC_00910 0.28 -1.3 162,083 + fimA MCAODC_00910 0.28 +0.1 162,083 + fimA MCAODC_00910 0.28 +0.1 162,084 - fimA MCAODC_00910 0.28 -0.0 162,084 - fimA MCAODC_00910 0.28 +1.1 162,109 - fimA MCAODC_00910 0.30 +0.4 162,137 + fimA MCAODC_00910 0.33 +1.4 162,138 - fimA MCAODC_00910 0.33 +0.5 162,138 - fimA MCAODC_00910 0.33 -3.3 162,138 - fimA MCAODC_00910 0.33 +1.0 162,151 - fimA MCAODC_00910 0.34 +1.0 162,151 - fimA MCAODC_00910 0.34 -2.1 162,151 - fimA MCAODC_00910 0.34 +0.4
Or see this region's nucleotide sequence