Experiment: Guanine 0.6617 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt A4249_RS10705 and A4249_RS10710 overlap by 7 nucleotides A4249_RS10710 and A4249_RS10715 are separated by 31 nucleotides A4249_RS10715 and A4249_RS10720 overlap by 4 nucleotides A4249_RS10720 and A4249_RS10725 are separated by 48 nucleotides
A4249_RS10705: A4249_RS10705 - imidazole glycerol phosphate synthase subunit HisH, at 2,152,517 to 2,153,119
_RS10705
A4249_RS10710: A4249_RS10710 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase, at 2,153,113 to 2,153,850
_RS10710
A4249_RS10715: A4249_RS10715 - imidazole glycerol phosphate synthase subunit HisF, at 2,153,882 to 2,154,649
_RS10715
A4249_RS10720: A4249_RS10720 - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE, at 2,154,646 to 2,155,269
_RS10720
A4249_RS10725: A4249_RS10725 - glucose-6-phosphate dehydrogenase, at 2,155,318 to 2,156,778
_RS10725
Position (kb)
2153
2154
2155 Strain fitness (log2 ratio)
-1
0
1
2 at 2152.935 kb on - strand, within A4249_RS10705 at 2152.935 kb on - strand, within A4249_RS10705 at 2152.935 kb on - strand, within A4249_RS10705 at 2152.935 kb on - strand, within A4249_RS10705 at 2152.935 kb on - strand, within A4249_RS10705 at 2152.935 kb on - strand, within A4249_RS10705 at 2152.940 kb on + strand, within A4249_RS10705 at 2152.941 kb on - strand, within A4249_RS10705 at 2152.941 kb on - strand, within A4249_RS10705 at 2152.997 kb on + strand, within A4249_RS10705 at 2152.998 kb on - strand, within A4249_RS10705 at 2153.122 kb on + strand at 2153.123 kb on - strand at 2153.194 kb on + strand, within A4249_RS10710 at 2153.195 kb on - strand, within A4249_RS10710 at 2153.797 kb on + strand at 2153.916 kb on + strand at 2153.916 kb on + strand at 2153.917 kb on - strand at 2153.917 kb on - strand at 2154.029 kb on + strand, within A4249_RS10715 at 2154.029 kb on + strand, within A4249_RS10715 at 2154.029 kb on + strand, within A4249_RS10715 at 2154.029 kb on + strand, within A4249_RS10715 at 2154.029 kb on + strand, within A4249_RS10715 at 2154.030 kb on - strand, within A4249_RS10715 at 2154.030 kb on - strand, within A4249_RS10715 at 2154.030 kb on - strand, within A4249_RS10715 at 2154.030 kb on - strand, within A4249_RS10715 at 2154.030 kb on - strand, within A4249_RS10715 at 2154.030 kb on - strand, within A4249_RS10715 at 2154.068 kb on + strand, within A4249_RS10715 at 2154.068 kb on + strand, within A4249_RS10715 at 2154.069 kb on - strand, within A4249_RS10715 at 2154.308 kb on + strand, within A4249_RS10715 at 2154.308 kb on + strand, within A4249_RS10715 at 2154.308 kb on + strand, within A4249_RS10715 at 2154.308 kb on + strand, within A4249_RS10715 at 2154.308 kb on + strand, within A4249_RS10715 at 2154.309 kb on - strand, within A4249_RS10715 at 2154.309 kb on - strand, within A4249_RS10715 at 2154.309 kb on - strand, within A4249_RS10715 at 2154.309 kb on - strand, within A4249_RS10715 at 2154.309 kb on - strand, within A4249_RS10715 at 2154.309 kb on - strand, within A4249_RS10715 at 2154.309 kb on - strand, within A4249_RS10715 at 2154.309 kb on - strand, within A4249_RS10715 at 2154.309 kb on - strand, within A4249_RS10715 at 2154.309 kb on - strand, within A4249_RS10715 at 2154.309 kb on - strand, within A4249_RS10715 at 2154.309 kb on - strand, within A4249_RS10715 at 2154.311 kb on - strand, within A4249_RS10715 at 2154.311 kb on - strand, within A4249_RS10715 at 2154.437 kb on + strand, within A4249_RS10715 at 2154.438 kb on - strand, within A4249_RS10715 at 2154.639 kb on + strand at 2154.639 kb on + strand at 2154.639 kb on + strand at 2154.639 kb on + strand at 2154.639 kb on + strand at 2154.639 kb on + strand at 2154.639 kb on + strand at 2154.639 kb on + strand at 2154.639 kb on + strand at 2154.639 kb on + strand at 2154.640 kb on - strand at 2154.640 kb on - strand at 2154.640 kb on - strand at 2154.640 kb on - strand at 2154.640 kb on - strand at 2154.640 kb on - strand at 2154.640 kb on - strand at 2154.640 kb on - strand at 2154.742 kb on + strand, within A4249_RS10720 at 2154.742 kb on + strand, within A4249_RS10720 at 2154.742 kb on + strand, within A4249_RS10720 at 2154.742 kb on + strand, within A4249_RS10720 at 2154.742 kb on + strand, within A4249_RS10720 at 2154.742 kb on + strand, within A4249_RS10720 at 2154.742 kb on + strand, within A4249_RS10720 at 2154.742 kb on + strand, within A4249_RS10720 at 2154.743 kb on - strand, within A4249_RS10720 at 2154.743 kb on - strand, within A4249_RS10720 at 2154.743 kb on - strand, within A4249_RS10720 at 2154.743 kb on - strand, within A4249_RS10720 at 2154.743 kb on - strand, within A4249_RS10720 at 2154.743 kb on - strand, within A4249_RS10720 at 2154.743 kb on - strand, within A4249_RS10720 at 2154.743 kb on - strand, within A4249_RS10720 at 2154.743 kb on - strand, within A4249_RS10720 at 2154.743 kb on - strand, within A4249_RS10720 at 2154.743 kb on - strand, within A4249_RS10720 at 2154.744 kb on + strand, within A4249_RS10720 at 2154.745 kb on - strand, within A4249_RS10720 at 2154.745 kb on - strand, within A4249_RS10720 at 2155.171 kb on + strand, within A4249_RS10720 at 2155.171 kb on + strand, within A4249_RS10720 at 2155.171 kb on + strand, within A4249_RS10720 at 2155.171 kb on + strand, within A4249_RS10720 at 2155.171 kb on + strand, within A4249_RS10720 at 2155.172 kb on - strand, within A4249_RS10720 at 2155.172 kb on - strand, within A4249_RS10720 at 2155.172 kb on - strand, within A4249_RS10720 at 2155.172 kb on - strand, within A4249_RS10720 at 2155.172 kb on - strand, within A4249_RS10720 at 2155.172 kb on - strand, within A4249_RS10720 at 2155.172 kb on - strand, within A4249_RS10720 at 2155.172 kb on - strand, within A4249_RS10720 at 2155.201 kb on - strand, within A4249_RS10720 at 2155.267 kb on + strand at 2155.274 kb on + strand at 2155.274 kb on + strand at 2155.274 kb on + strand at 2155.274 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Guanine 0.6617 mM (C) remove 2,152,935 - A4249_RS10705 0.69 +0.5 2,152,935 - A4249_RS10705 0.69 -0.6 2,152,935 - A4249_RS10705 0.69 +0.2 2,152,935 - A4249_RS10705 0.69 -0.8 2,152,935 - A4249_RS10705 0.69 -0.9 2,152,935 - A4249_RS10705 0.69 +0.9 2,152,940 + A4249_RS10705 0.70 +0.6 2,152,941 - A4249_RS10705 0.70 -0.1 2,152,941 - A4249_RS10705 0.70 -0.1 2,152,997 + A4249_RS10705 0.80 +1.1 2,152,998 - A4249_RS10705 0.80 -1.3 2,153,122 + +0.1 2,153,123 - -0.3 2,153,194 + A4249_RS10710 0.11 -0.0 2,153,195 - A4249_RS10710 0.11 +0.4 2,153,797 + +0.6 2,153,916 + -0.1 2,153,916 + +0.4 2,153,917 - -1.0 2,153,917 - +0.3 2,154,029 + A4249_RS10715 0.19 +0.1 2,154,029 + A4249_RS10715 0.19 +0.2 2,154,029 + A4249_RS10715 0.19 -0.1 2,154,029 + A4249_RS10715 0.19 -0.4 2,154,029 + A4249_RS10715 0.19 -0.3 2,154,030 - A4249_RS10715 0.19 -0.4 2,154,030 - A4249_RS10715 0.19 -0.3 2,154,030 - A4249_RS10715 0.19 +0.1 2,154,030 - A4249_RS10715 0.19 -0.8 2,154,030 - A4249_RS10715 0.19 -1.1 2,154,030 - A4249_RS10715 0.19 -0.2 2,154,068 + A4249_RS10715 0.24 -0.5 2,154,068 + A4249_RS10715 0.24 -0.2 2,154,069 - A4249_RS10715 0.24 -1.3 2,154,308 + A4249_RS10715 0.55 -0.1 2,154,308 + A4249_RS10715 0.55 -1.1 2,154,308 + A4249_RS10715 0.55 -0.5 2,154,308 + A4249_RS10715 0.55 +0.7 2,154,308 + A4249_RS10715 0.55 -0.6 2,154,309 - A4249_RS10715 0.56 +0.3 2,154,309 - A4249_RS10715 0.56 -1.0 2,154,309 - A4249_RS10715 0.56 +0.1 2,154,309 - A4249_RS10715 0.56 +0.3 2,154,309 - A4249_RS10715 0.56 +0.0 2,154,309 - A4249_RS10715 0.56 -0.2 2,154,309 - A4249_RS10715 0.56 -0.2 2,154,309 - A4249_RS10715 0.56 -0.6 2,154,309 - A4249_RS10715 0.56 +0.6 2,154,309 - A4249_RS10715 0.56 -0.9 2,154,309 - A4249_RS10715 0.56 -1.2 2,154,309 - A4249_RS10715 0.56 -0.6 2,154,311 - A4249_RS10715 0.56 -0.7 2,154,311 - A4249_RS10715 0.56 -0.6 2,154,437 + A4249_RS10715 0.72 +0.2 2,154,438 - A4249_RS10715 0.72 +1.1 2,154,639 + +2.4 2,154,639 + +1.6 2,154,639 + +0.8 2,154,639 + -1.0 2,154,639 + +0.4 2,154,639 + -0.1 2,154,639 + -0.5 2,154,639 + +0.6 2,154,639 + -0.4 2,154,639 + -0.1 2,154,640 - +0.7 2,154,640 - +0.6 2,154,640 - +0.2 2,154,640 - +1.4 2,154,640 - +0.2 2,154,640 - +0.2 2,154,640 - +1.5 2,154,640 - -0.4 2,154,742 + A4249_RS10720 0.15 -0.4 2,154,742 + A4249_RS10720 0.15 -0.2 2,154,742 + A4249_RS10720 0.15 -1.1 2,154,742 + A4249_RS10720 0.15 -0.1 2,154,742 + A4249_RS10720 0.15 -1.0 2,154,742 + A4249_RS10720 0.15 +0.4 2,154,742 + A4249_RS10720 0.15 +0.8 2,154,742 + A4249_RS10720 0.15 -0.4 2,154,743 - A4249_RS10720 0.16 +0.1 2,154,743 - A4249_RS10720 0.16 -0.1 2,154,743 - A4249_RS10720 0.16 -0.5 2,154,743 - A4249_RS10720 0.16 -0.8 2,154,743 - A4249_RS10720 0.16 +0.1 2,154,743 - A4249_RS10720 0.16 -0.1 2,154,743 - A4249_RS10720 0.16 +0.5 2,154,743 - A4249_RS10720 0.16 -0.5 2,154,743 - A4249_RS10720 0.16 +0.2 2,154,743 - A4249_RS10720 0.16 +0.3 2,154,743 - A4249_RS10720 0.16 -0.8 2,154,744 + A4249_RS10720 0.16 +1.3 2,154,745 - A4249_RS10720 0.16 -0.1 2,154,745 - A4249_RS10720 0.16 -1.1 2,155,171 + A4249_RS10720 0.84 +1.3 2,155,171 + A4249_RS10720 0.84 -0.0 2,155,171 + A4249_RS10720 0.84 +0.1 2,155,171 + A4249_RS10720 0.84 -0.3 2,155,171 + A4249_RS10720 0.84 -0.3 2,155,172 - A4249_RS10720 0.84 -0.0 2,155,172 - A4249_RS10720 0.84 -0.3 2,155,172 - A4249_RS10720 0.84 -0.3 2,155,172 - A4249_RS10720 0.84 -0.2 2,155,172 - A4249_RS10720 0.84 -0.2 2,155,172 - A4249_RS10720 0.84 +0.4 2,155,172 - A4249_RS10720 0.84 -0.5 2,155,172 - A4249_RS10720 0.84 +0.3 2,155,201 - A4249_RS10720 0.89 -0.6 2,155,267 + -0.1 2,155,274 + +0.9 2,155,274 + -0.6 2,155,274 + +0.2 2,155,274 + -0.8
Or see this region's nucleotide sequence