Strain Fitness in Escherichia coli ECRC102 around NIAGMN_27695

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnarH and narG overlap by 4 nucleotidesnarG and narK are separated by 392 nucleotides NIAGMN_27690: narH - nitrate reductase subunit beta, at 5,363,425 to 5,364,963 narH NIAGMN_27695: narG - nitrate reductase subunit alpha, at 5,364,960 to 5,368,703 narG NIAGMN_27700: narK - nitrate transporter NarK, at 5,369,096 to 5,370,487 narK Position (kb) 5364 5365 5366 5367 5368 5369Strain fitness (log2 ratio) -2 -1 0 1 2 3at 5364.459 kb on - strand, within narHat 5364.921 kb on + strandat 5364.979 kb on - strandat 5365.059 kb on + strandat 5365.077 kb on - strandat 5365.200 kb on - strandat 5366.224 kb on + strand, within narGat 5366.225 kb on - strand, within narGat 5366.558 kb on + strand, within narGat 5367.194 kb on + strand, within narGat 5367.214 kb on + strand, within narGat 5367.567 kb on - strand, within narGat 5368.051 kb on + strand, within narGat 5368.088 kb on - strand, within narGat 5368.178 kb on - strand, within narGat 5368.290 kb on - strand, within narGat 5368.471 kb on + strandat 5368.923 kb on + strandat 5369.152 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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5,364,459 - narH NIAGMN_27690 0.67 -0.6
5,364,921 + -0.7
5,364,979 - -1.8
5,365,059 + -0.3
5,365,077 - +0.6
5,365,200 - +0.1
5,366,224 + narG NIAGMN_27695 0.34 -0.5
5,366,225 - narG NIAGMN_27695 0.34 -0.5
5,366,558 + narG NIAGMN_27695 0.43 -0.1
5,367,194 + narG NIAGMN_27695 0.60 -2.0
5,367,214 + narG NIAGMN_27695 0.60 -0.7
5,367,567 - narG NIAGMN_27695 0.70 +0.5
5,368,051 + narG NIAGMN_27695 0.83 +0.4
5,368,088 - narG NIAGMN_27695 0.84 +1.3
5,368,178 - narG NIAGMN_27695 0.86 -0.9
5,368,290 - narG NIAGMN_27695 0.89 -2.1
5,368,471 + -0.7
5,368,923 + -0.2
5,369,152 - +3.1

Or see this region's nucleotide sequence